β-lactamases - Normal peptidases?

M. I. Page, S. Buckwell

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

The mechanism of the β-lactamase catalysed hydrolysis of β-lactam antibiotics is discussed and compared with that of normal serine proteases. There is little evidence for a basic group on the enzyme acting as a general base catalyst. The effect of chain length on the kinetic parameters for the hydrolysis of 6-alkylpenicillins is described. The expulsion of the leaving group at C3′ in cephalosporins is reversible and is not catalysed by β-lactamase.

Original languageEnglish
Pages (from-to)241-248
Number of pages8
JournalAdvances in the Biosciences
Volume65
Issue numberC
Publication statusPublished - 1 Dec 1987

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Hydrolysis
Peptide Hydrolases
Lactams
Serine Proteases
Cephalosporins
Chain length
Kinetic parameters
Anti-Bacterial Agents
Catalysts
Enzymes

Cite this

Page, M. I., & Buckwell, S. (1987). β-lactamases - Normal peptidases? Advances in the Biosciences, 65(C), 241-248.
Page, M. I. ; Buckwell, S. / β-lactamases - Normal peptidases?. In: Advances in the Biosciences. 1987 ; Vol. 65, No. C. pp. 241-248.
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Page, MI & Buckwell, S 1987, 'β-lactamases - Normal peptidases?', Advances in the Biosciences, vol. 65, no. C, pp. 241-248.

β-lactamases - Normal peptidases? / Page, M. I.; Buckwell, S.

In: Advances in the Biosciences, Vol. 65, No. C, 01.12.1987, p. 241-248.

Research output: Contribution to journalArticle

TY - JOUR

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AU - Page, M. I.

AU - Buckwell, S.

PY - 1987/12/1

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N2 - The mechanism of the β-lactamase catalysed hydrolysis of β-lactam antibiotics is discussed and compared with that of normal serine proteases. There is little evidence for a basic group on the enzyme acting as a general base catalyst. The effect of chain length on the kinetic parameters for the hydrolysis of 6-alkylpenicillins is described. The expulsion of the leaving group at C3′ in cephalosporins is reversible and is not catalysed by β-lactamase.

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Page MI, Buckwell S. β-lactamases - Normal peptidases? Advances in the Biosciences. 1987 Dec 1;65(C):241-248.