The mechanism of the β-lactamase catalysed hydrolysis of β-lactam antibiotics is discussed and compared with that of normal serine proteases. There is little evidence for a basic group on the enzyme acting as a general base catalyst. The effect of chain length on the kinetic parameters for the hydrolysis of 6-alkylpenicillins is described. The expulsion of the leaving group at C3′ in cephalosporins is reversible and is not catalysed by β-lactamase.
|Number of pages||8|
|Journal||Advances in the Biosciences|
|Publication status||Published - 1 Dec 1987|