β-lactamases - Normal peptidases?

M. I. Page; S. Buckwell

β-lactamases - Normal peptidases?

M. I. Page, S. Buckwell

School of Applied Sciences

Research output: Contribution to journal › Article › peer-review

3 Citations (Scopus)

Abstract

The mechanism of the β-lactamase catalysed hydrolysis of β-lactam antibiotics is discussed and compared with that of normal serine proteases. There is little evidence for a basic group on the enzyme acting as a general base catalyst. The effect of chain length on the kinetic parameters for the hydrolysis of 6-alkylpenicillins is described. The expulsion of the leaving group at C3′ in cephalosporins is reversible and is not catalysed by β-lactamase.

Original language	English
Pages (from-to)	241-248
Number of pages	8
Journal	Advances in the Biosciences
Volume	65
Issue number	C
Publication status	Published - 1 Dec 1987

Cite this

@article{095c45a95b6142ed86e86a48c6c99f8c,

title = "β-lactamases - Normal peptidases?",

abstract = "The mechanism of the β-lactamase catalysed hydrolysis of β-lactam antibiotics is discussed and compared with that of normal serine proteases. There is little evidence for a basic group on the enzyme acting as a general base catalyst. The effect of chain length on the kinetic parameters for the hydrolysis of 6-alkylpenicillins is described. The expulsion of the leaving group at C3′ in cephalosporins is reversible and is not catalysed by β-lactamase.",

keywords = "β-lactam antibiotics, β-lactamase, kinetics, mechanism, substituent effects",

author = "Page, \{M. I.\} and S. Buckwell",

year = "1987",

month = dec,

day = "1",

language = "English",

volume = "65",

pages = "241--248",

journal = "Advances in the Biosciences",

issn = "0065-3446",