A bio-electrochemical sensing platform for glucose based on irreversible, non-covalent pi-pi functionalization of graphene produced via a novel, green synthesis method

Joanna Su Yuin Chia, Michelle T.T. Tan, Poi Sim Khiew, Jit Kai Chin, Chiu Wee Siong

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

In this work, pristine graphene was produced through a novel single step exfoliation of graphite in mild sonochemical alcohol-water treatment. The developed green synthesis approach successfully eradicates issues associated with conventional methods which use organic solvents, acids and oxidizers, leaving undesirable functional groups attached to the graphene surface. Results from cyclic voltammetry and amperometric analysis showed a wide linear range up to 5 mM and sensitivity improvements of more than 22 times in comparison to the control sample. Subsequently, an electrochemical glucose biosensor was fabricated by the immobilization of glucose oxidase (GOx) via bi-functional linkers. This reliable surface modification method provides irreversible non-covalent bonding between graphene and the enzymatic amide groups, while preserving the sp2 graphene structure, whilst promoting better electron transfer kinetics between the FAD/FADH2 redox sites of GOx at the modified electrode surface. The fabricated biosensor exhibited satisfactory long-term stability, reproducibility and high selectivity for glucose detection and showed significant improvements when compared to unmodified electrodes.

LanguageEnglish
Pages558-565
Number of pages8
JournalSensors and Actuators, B: Chemical
Volume210
DOIs
Publication statusPublished - Apr 2015
Externally publishedYes

Fingerprint

Graphite
glucose
Graphene
Glucose
graphene
platforms
Glucose oxidase
oxidase
synthesis
Glucose Oxidase
bioinstrumentation
Biosensors
Enzyme kinetics
Electrodes
water treatment
electrodes
oxidizers
immobilization
Water treatment
Amides

Cite this

@article{e8c2058c93374e5c82ff4ec5aec76d14,
title = "A bio-electrochemical sensing platform for glucose based on irreversible, non-covalent pi-pi functionalization of graphene produced via a novel, green synthesis method",
abstract = "In this work, pristine graphene was produced through a novel single step exfoliation of graphite in mild sonochemical alcohol-water treatment. The developed green synthesis approach successfully eradicates issues associated with conventional methods which use organic solvents, acids and oxidizers, leaving undesirable functional groups attached to the graphene surface. Results from cyclic voltammetry and amperometric analysis showed a wide linear range up to 5 mM and sensitivity improvements of more than 22 times in comparison to the control sample. Subsequently, an electrochemical glucose biosensor was fabricated by the immobilization of glucose oxidase (GOx) via bi-functional linkers. This reliable surface modification method provides irreversible non-covalent bonding between graphene and the enzymatic amide groups, while preserving the sp2 graphene structure, whilst promoting better electron transfer kinetics between the FAD/FADH2 redox sites of GOx at the modified electrode surface. The fabricated biosensor exhibited satisfactory long-term stability, reproducibility and high selectivity for glucose detection and showed significant improvements when compared to unmodified electrodes.",
keywords = "Amperometric sensors, Atomic force microscopy, Cyclic voltammetry, Electrochemical biosensors, Flow injection analysis, Graphene glucose sensors",
author = "Chia, {Joanna Su Yuin} and Tan, {Michelle T.T.} and Khiew, {Poi Sim} and Chin, {Jit Kai} and Siong, {Chiu Wee}",
year = "2015",
month = "4",
doi = "10.1016/j.snb.2015.01.023",
language = "English",
volume = "210",
pages = "558--565",
journal = "Sensors and Actuators, B: Chemical",
issn = "0925-4005",
publisher = "Elsevier",

}

A bio-electrochemical sensing platform for glucose based on irreversible, non-covalent pi-pi functionalization of graphene produced via a novel, green synthesis method. / Chia, Joanna Su Yuin; Tan, Michelle T.T.; Khiew, Poi Sim; Chin, Jit Kai; Siong, Chiu Wee.

In: Sensors and Actuators, B: Chemical, Vol. 210, 04.2015, p. 558-565.

Research output: Contribution to journalArticle

TY - JOUR

T1 - A bio-electrochemical sensing platform for glucose based on irreversible, non-covalent pi-pi functionalization of graphene produced via a novel, green synthesis method

AU - Chia, Joanna Su Yuin

AU - Tan, Michelle T.T.

AU - Khiew, Poi Sim

AU - Chin, Jit Kai

AU - Siong, Chiu Wee

PY - 2015/4

Y1 - 2015/4

N2 - In this work, pristine graphene was produced through a novel single step exfoliation of graphite in mild sonochemical alcohol-water treatment. The developed green synthesis approach successfully eradicates issues associated with conventional methods which use organic solvents, acids and oxidizers, leaving undesirable functional groups attached to the graphene surface. Results from cyclic voltammetry and amperometric analysis showed a wide linear range up to 5 mM and sensitivity improvements of more than 22 times in comparison to the control sample. Subsequently, an electrochemical glucose biosensor was fabricated by the immobilization of glucose oxidase (GOx) via bi-functional linkers. This reliable surface modification method provides irreversible non-covalent bonding between graphene and the enzymatic amide groups, while preserving the sp2 graphene structure, whilst promoting better electron transfer kinetics between the FAD/FADH2 redox sites of GOx at the modified electrode surface. The fabricated biosensor exhibited satisfactory long-term stability, reproducibility and high selectivity for glucose detection and showed significant improvements when compared to unmodified electrodes.

AB - In this work, pristine graphene was produced through a novel single step exfoliation of graphite in mild sonochemical alcohol-water treatment. The developed green synthesis approach successfully eradicates issues associated with conventional methods which use organic solvents, acids and oxidizers, leaving undesirable functional groups attached to the graphene surface. Results from cyclic voltammetry and amperometric analysis showed a wide linear range up to 5 mM and sensitivity improvements of more than 22 times in comparison to the control sample. Subsequently, an electrochemical glucose biosensor was fabricated by the immobilization of glucose oxidase (GOx) via bi-functional linkers. This reliable surface modification method provides irreversible non-covalent bonding between graphene and the enzymatic amide groups, while preserving the sp2 graphene structure, whilst promoting better electron transfer kinetics between the FAD/FADH2 redox sites of GOx at the modified electrode surface. The fabricated biosensor exhibited satisfactory long-term stability, reproducibility and high selectivity for glucose detection and showed significant improvements when compared to unmodified electrodes.

KW - Amperometric sensors

KW - Atomic force microscopy

KW - Cyclic voltammetry

KW - Electrochemical biosensors

KW - Flow injection analysis

KW - Graphene glucose sensors

UR - http://www.scopus.com/inward/record.url?scp=84921719343&partnerID=8YFLogxK

U2 - 10.1016/j.snb.2015.01.023

DO - 10.1016/j.snb.2015.01.023

M3 - Article

VL - 210

SP - 558

EP - 565

JO - Sensors and Actuators, B: Chemical

T2 - Sensors and Actuators, B: Chemical

JF - Sensors and Actuators, B: Chemical

SN - 0925-4005

ER -