TY - JOUR
T1 - A Concerted Enzymatic and Bioorthogonal Approach for Extra- and Intracellular Activation of Environment-Sensitive Ruthenium(II)-Based Imaging Probes and Photosensitizers
AU - Shum, Justin
AU - Lee, Lawrence Cho Cheung
AU - Chiang, Michael Wai Lun
AU - Lam, Yun Wah
AU - Lo, Kenneth Kam Wing
N1 - Funding Information:
We thank the Hong Kong Research Grants Council (Project Nos. CityU 11302820, CityU 11301121, CityU 11317022, T42‐103/16‐N, C6014‐20 W, and C7075‐21GF) and the Hong Kong Research Grants Council, National Natural Science Foundation of China (Project No. N_CityU104/21) for financial support. We also thank the funding support from “Laboratory for Synthetic Chemistry and Chemical Biology” under the Health@InnoHK Program launched by Innovation and Technology Commission, The Government of Hong Kong SAR, P. R. China. J.S. acknowledges the receipt of a Postgraduate Studentship administered by City University of Hong Kong.
Funding Information:
We thank the Hong Kong Research Grants Council (Project Nos. CityU 11302820, CityU 11301121, CityU 11317022, T42-103/16-N, C6014-20 W, and C7075-21GF) and the Hong Kong Research Grants Council, National Natural Science Foundation of China (Project No. N_CityU104/21) for financial support. We also thank the funding support from “Laboratory for Synthetic Chemistry and Chemical Biology” under the Health@InnoHK Program launched by Innovation and Technology Commission, The Government of Hong Kong SAR, P. R. China. J.S. acknowledges the receipt of a Postgraduate Studentship administered by City University of Hong Kong.
Publisher Copyright:
© 2023 Wiley-VCH GmbH.
PY - 2023/7/17
Y1 - 2023/7/17
N2 - In this article, we report a novel targeting strategy involving the combination of an enzyme-instructed self-assembly (EISA) moiety and a strained cycloalkyne to generate large accumulation of bioorthogonal sites in cancer cells. These bioorthogonal sites can serve as activation triggers in different regions for transition metal-based probes, which are new ruthenium(II) complexes carrying a tetrazine unit for controllable phosphorescence and singlet oxygen generation. Importantly, the environment-sensitive emission of the complexes can be further enhanced in the hydrophobic regions offered by the large supramolecular assemblies, which is highly advantageous to biological imaging. Additionally, the (photo)cytotoxicity of the large supramolecular assemblies containing the complexes was investigated, and the results illustrate that cellular localization (extracellular and intracellular) imposes a profound impact on the efficiencies of photosensitizers.
AB - In this article, we report a novel targeting strategy involving the combination of an enzyme-instructed self-assembly (EISA) moiety and a strained cycloalkyne to generate large accumulation of bioorthogonal sites in cancer cells. These bioorthogonal sites can serve as activation triggers in different regions for transition metal-based probes, which are new ruthenium(II) complexes carrying a tetrazine unit for controllable phosphorescence and singlet oxygen generation. Importantly, the environment-sensitive emission of the complexes can be further enhanced in the hydrophobic regions offered by the large supramolecular assemblies, which is highly advantageous to biological imaging. Additionally, the (photo)cytotoxicity of the large supramolecular assemblies containing the complexes was investigated, and the results illustrate that cellular localization (extracellular and intracellular) imposes a profound impact on the efficiencies of photosensitizers.
KW - Bioorthogonal
KW - Enzyme-Instructed Self-Assembly
KW - Imaging Agents
KW - Photosensitizers
KW - Ruthenium
UR - http://www.scopus.com/inward/record.url?scp=85161668489&partnerID=8YFLogxK
U2 - 10.1002/anie.202303931
DO - 10.1002/anie.202303931
M3 - Article
AN - SCOPUS:85161668489
VL - 62
JO - Angewandte Chemie - International Edition
JF - Angewandte Chemie - International Edition
SN - 1433-7851
IS - 29
M1 - e202303931
ER -