A copolymer analysis approach to estimate the neutral sugar distribution of sugar beet pectin using size exclusion chromatography

Gordon A. Morris, Marie Christine Ralet

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Partially degraded sugar beet (Beta vulgaris) pectins were characterised in terms of galacturonic acid, neutral sugar and ferulic acids contents. It was shown that the total neutral sugar content is correlated with the ferulic acid content. One pectin (C) was further characterised by size exclusion chromatography coupled to refractive index and UV detectors (SEC-RI-UV). This gave the opportunity to estimate how the ferulic acid and neutral sugar contents changed with hydrodynamic radius. Pectin C was found to be heterogeneous in composition with neutral sugar-rich fractions of both high and low hydrodynamic radii. A neutral sugar-poor fraction was found at intermediate hydrodynamic radii.

Original languageEnglish
Pages (from-to)1139-1143
Number of pages5
JournalCarbohydrate Polymers
Volume87
Issue number2
Early online date31 Aug 2011
DOIs
Publication statusPublished - 15 Jan 2012

Fingerprint

ferulic acid
Sugar beets
Size exclusion chromatography
Sugar (sucrose)
Sugars
Copolymers
Hydrodynamics
Acids
Pectins
Ultraviolet detectors
Sugar Acids
Refractive index
pectin
Chemical analysis

Cite this

@article{86da85e5d942468792c50423cf19954a,
title = "A copolymer analysis approach to estimate the neutral sugar distribution of sugar beet pectin using size exclusion chromatography",
abstract = "Partially degraded sugar beet (Beta vulgaris) pectins were characterised in terms of galacturonic acid, neutral sugar and ferulic acids contents. It was shown that the total neutral sugar content is correlated with the ferulic acid content. One pectin (C) was further characterised by size exclusion chromatography coupled to refractive index and UV detectors (SEC-RI-UV). This gave the opportunity to estimate how the ferulic acid and neutral sugar contents changed with hydrodynamic radius. Pectin C was found to be heterogeneous in composition with neutral sugar-rich fractions of both high and low hydrodynamic radii. A neutral sugar-poor fraction was found at intermediate hydrodynamic radii.",
keywords = "Ferulic acid distribution, Neutral sugar distribution, RI, Sugar beet (Beta vulgaris) pectin, UV",
author = "Morris, {Gordon A.} and Ralet, {Marie Christine}",
year = "2012",
month = "1",
day = "15",
doi = "10.1016/j.carbpol.2011.08.077",
language = "English",
volume = "87",
pages = "1139--1143",
journal = "Carbohydrate Polymers",
issn = "0144-8617",
publisher = "Elsevier Limited",
number = "2",

}

A copolymer analysis approach to estimate the neutral sugar distribution of sugar beet pectin using size exclusion chromatography. / Morris, Gordon A.; Ralet, Marie Christine.

In: Carbohydrate Polymers, Vol. 87, No. 2, 15.01.2012, p. 1139-1143.

Research output: Contribution to journalArticle

TY - JOUR

T1 - A copolymer analysis approach to estimate the neutral sugar distribution of sugar beet pectin using size exclusion chromatography

AU - Morris, Gordon A.

AU - Ralet, Marie Christine

PY - 2012/1/15

Y1 - 2012/1/15

N2 - Partially degraded sugar beet (Beta vulgaris) pectins were characterised in terms of galacturonic acid, neutral sugar and ferulic acids contents. It was shown that the total neutral sugar content is correlated with the ferulic acid content. One pectin (C) was further characterised by size exclusion chromatography coupled to refractive index and UV detectors (SEC-RI-UV). This gave the opportunity to estimate how the ferulic acid and neutral sugar contents changed with hydrodynamic radius. Pectin C was found to be heterogeneous in composition with neutral sugar-rich fractions of both high and low hydrodynamic radii. A neutral sugar-poor fraction was found at intermediate hydrodynamic radii.

AB - Partially degraded sugar beet (Beta vulgaris) pectins were characterised in terms of galacturonic acid, neutral sugar and ferulic acids contents. It was shown that the total neutral sugar content is correlated with the ferulic acid content. One pectin (C) was further characterised by size exclusion chromatography coupled to refractive index and UV detectors (SEC-RI-UV). This gave the opportunity to estimate how the ferulic acid and neutral sugar contents changed with hydrodynamic radius. Pectin C was found to be heterogeneous in composition with neutral sugar-rich fractions of both high and low hydrodynamic radii. A neutral sugar-poor fraction was found at intermediate hydrodynamic radii.

KW - Ferulic acid distribution

KW - Neutral sugar distribution

KW - RI

KW - Sugar beet (Beta vulgaris) pectin

KW - UV

UR - http://www.scopus.com/inward/record.url?scp=81255134465&partnerID=8YFLogxK

U2 - 10.1016/j.carbpol.2011.08.077

DO - 10.1016/j.carbpol.2011.08.077

M3 - Article

VL - 87

SP - 1139

EP - 1143

JO - Carbohydrate Polymers

JF - Carbohydrate Polymers

SN - 0144-8617

IS - 2

ER -