A new HPLC method for simultaneously measuring chloride, sugars, organic acids and alcohols in food samples

Abdelrahman Saleh Zaky, Nattha Pensupa, Áurea Andrade-eiroa, Gregory A. Tucker, Chenyu Du

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

This paper introduces an original, rapid, efficient and reliable HPLC method for the accurate and simultaneous quantification (g/L) of chloride in samples containing sugars, organic acids and alcohols. Separation was achieved using a HI-Plex H column at 35 °C, with H2SO4 (0.005 N) as the mobile phase at a flow rate of 0.4 mL/min. The column effluent was monitored by a Refractive Index (RI) detector. A linear response was achieved over NaCl concentrations of 0.25–2.5 g/L and 5–40 g/L. The analytical method inter- and intra-run accuracy and precision were better than ±10.0%. Investigating the mechanism of detection using different chloride and sodium s reviled that this method can be used for determining the total concentration of chloride salts when in suspension. This method was successfully applied to 15 samples of commercial food products and the salt content obtained from this method was compared with 3 other methods for salt determination. The (HI-Plex H) column was designed for determining the concentrations of sugars, organic acids and alcohols when in solution. Hence, application of our new methodology would allow the determination of sugars, alcohols and organic acids in samples derived from seawater-based fermentation media as well as samples from salty food and dairy products.
LanguageEnglish
Pages25-33
Number of pages9
JournalJournal of Food Composition and Analysis
Volume56
Early online date12 Dec 2016
DOIs
Publication statusPublished - 1 Mar 2017
Externally publishedYes

Fingerprint

Sugar Alcohols
Sugar Acids
organic acids and salts
Chlorides
chlorides
foods
alcohols
high performance liquid chromatography
High Pressure Liquid Chromatography
sugars
Food
Salts
sampling
methodology
salts
sugar alcohols
Refractometry
Dairy Products
refractive index
salt content

Cite this

Zaky, Abdelrahman Saleh ; Pensupa, Nattha ; Andrade-eiroa, Áurea ; Tucker, Gregory A. ; Du, Chenyu. / A new HPLC method for simultaneously measuring chloride, sugars, organic acids and alcohols in food samples. In: Journal of Food Composition and Analysis. 2017 ; Vol. 56. pp. 25-33.
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abstract = "This paper introduces an original, rapid, efficient and reliable HPLC method for the accurate and simultaneous quantification (g/L) of chloride in samples containing sugars, organic acids and alcohols. Separation was achieved using a HI-Plex H column at 35 °C, with H2SO4 (0.005 N) as the mobile phase at a flow rate of 0.4 mL/min. The column effluent was monitored by a Refractive Index (RI) detector. A linear response was achieved over NaCl concentrations of 0.25–2.5 g/L and 5–40 g/L. The analytical method inter- and intra-run accuracy and precision were better than ±10.0{\%}. Investigating the mechanism of detection using different chloride and sodium s reviled that this method can be used for determining the total concentration of chloride salts when in suspension. This method was successfully applied to 15 samples of commercial food products and the salt content obtained from this method was compared with 3 other methods for salt determination. The (HI-Plex H) column was designed for determining the concentrations of sugars, organic acids and alcohols when in solution. Hence, application of our new methodology would allow the determination of sugars, alcohols and organic acids in samples derived from seawater-based fermentation media as well as samples from salty food and dairy products.",
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A new HPLC method for simultaneously measuring chloride, sugars, organic acids and alcohols in food samples. / Zaky, Abdelrahman Saleh; Pensupa, Nattha; Andrade-eiroa, Áurea; Tucker, Gregory A.; Du, Chenyu.

In: Journal of Food Composition and Analysis, Vol. 56, 01.03.2017, p. 25-33.

Research output: Contribution to journalArticle

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T1 - A new HPLC method for simultaneously measuring chloride, sugars, organic acids and alcohols in food samples

AU - Zaky, Abdelrahman Saleh

AU - Pensupa, Nattha

AU - Andrade-eiroa, Áurea

AU - Tucker, Gregory A.

AU - Du, Chenyu

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N2 - This paper introduces an original, rapid, efficient and reliable HPLC method for the accurate and simultaneous quantification (g/L) of chloride in samples containing sugars, organic acids and alcohols. Separation was achieved using a HI-Plex H column at 35 °C, with H2SO4 (0.005 N) as the mobile phase at a flow rate of 0.4 mL/min. The column effluent was monitored by a Refractive Index (RI) detector. A linear response was achieved over NaCl concentrations of 0.25–2.5 g/L and 5–40 g/L. The analytical method inter- and intra-run accuracy and precision were better than ±10.0%. Investigating the mechanism of detection using different chloride and sodium s reviled that this method can be used for determining the total concentration of chloride salts when in suspension. This method was successfully applied to 15 samples of commercial food products and the salt content obtained from this method was compared with 3 other methods for salt determination. The (HI-Plex H) column was designed for determining the concentrations of sugars, organic acids and alcohols when in solution. Hence, application of our new methodology would allow the determination of sugars, alcohols and organic acids in samples derived from seawater-based fermentation media as well as samples from salty food and dairy products.

AB - This paper introduces an original, rapid, efficient and reliable HPLC method for the accurate and simultaneous quantification (g/L) of chloride in samples containing sugars, organic acids and alcohols. Separation was achieved using a HI-Plex H column at 35 °C, with H2SO4 (0.005 N) as the mobile phase at a flow rate of 0.4 mL/min. The column effluent was monitored by a Refractive Index (RI) detector. A linear response was achieved over NaCl concentrations of 0.25–2.5 g/L and 5–40 g/L. The analytical method inter- and intra-run accuracy and precision were better than ±10.0%. Investigating the mechanism of detection using different chloride and sodium s reviled that this method can be used for determining the total concentration of chloride salts when in suspension. This method was successfully applied to 15 samples of commercial food products and the salt content obtained from this method was compared with 3 other methods for salt determination. The (HI-Plex H) column was designed for determining the concentrations of sugars, organic acids and alcohols when in solution. Hence, application of our new methodology would allow the determination of sugars, alcohols and organic acids in samples derived from seawater-based fermentation media as well as samples from salty food and dairy products.

KW - Food analysis

KW - Food composition

KW - High Performance Liquid Chromatography (HPLC)

KW - Cl− Determination

KW - Sugar analysis

KW - Hi-Plex H column

KW - Seawater

KW - Salts meter

KW - Ion chromatography (IC)

KW - Flame photometer (FP)

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