A novel workflow for three-dimensional analysis of tumour cell migration

Sophie Ketchen, Arndt Rohwedder, Sabine Knipp, Filomena Esteves, Nina Struve, Michelle Peckham, John Ladbury, Alistair Curd, Susan Short, Anke Bruning-Richardson

Research output: Contribution to journalArticlepeer-review

4 Citations (Scopus)


The limitations of two-dimensional analysis in three-dimensional (3D) cellular imaging impair the accuracy of research findings in biological studies. Here, we report a novel 3D approach to acquisition, analysis and interpretation of tumour spheroid images. Our research interest in mesenchymal–amoeboid transition led to the development of a workflow incorporating the generation and analysis of 3D data with instant structured illumination microscopy and a new ImageJ plugin.
Original languageEnglish
Article number20190070
Number of pages6
JournalInterface Focus
Issue number2
Early online date14 Feb 2020
Publication statusPublished - 6 Apr 2020


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