The limitations of two-dimensional analysis in three-dimensional (3D) cellular imaging impair the accuracy of research findings in biological studies. Here, we report a novel 3D approach to acquisition, analysis and interpretation of tumour spheroid images. Our research interest in mesenchymal–amoeboid transition led to the development of a workflow incorporating the generation and analysis of 3D data with instant structured illumination microscopy and a new ImageJ plugin.
Ketchen, S., Rohwedder, A., Knipp, S., Esteves, F., Struve, N., Peckham, M., ... Bruning-Richardson, A. (2020). A novel workflow for three-dimensional analysis of tumour cell migration. Interface Focus, 10(2), . https://doi.org/10.1098/rsfs.2019.0070