A novel workflow for three-dimensional analysis of tumour cell migration

Sophie Ketchen; Arndt Rohwedder; Sabine Knipp; Filomena Esteves; Nina Struve; Michelle  Peckham; John Ladbury; Alistair Curd; Susan Short; Anke Bruning-Richardson

doi:10.1098/rsfs.2019.0070

A novel workflow for three-dimensional analysis of tumour cell migration

Sophie Ketchen, Arndt Rohwedder, Sabine Knipp, Filomena Esteves, Nina Struve, Michelle Peckham, John Ladbury, Alistair Curd, Susan Short, Anke Bruning-Richardson

Research output: Contribution to journal › Article › peer-review

5 Citations (Scopus)

Abstract

The limitations of two-dimensional analysis in three-dimensional (3D) cellular imaging impair the accuracy of research findings in biological studies. Here, we report a novel 3D approach to acquisition, analysis and interpretation of tumour spheroid images. Our research interest in mesenchymal–amoeboid transition led to the development of a workflow incorporating the generation and analysis of 3D data with instant structured illumination microscopy and a new ImageJ plugin.

Original language	English
Article number	20190070
Number of pages	6
Journal	Interface Focus
Volume	10
Issue number	2
Early online date	14 Feb 2020
DOIs	https://doi.org/10.1098/rsfs.2019.0070
Publication status	Published - 6 Apr 2020

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title = "A novel workflow for three-dimensional analysis of tumour cell migration",

abstract = "The limitations of two-dimensional analysis in three-dimensional (3D) cellular imaging impair the accuracy of research findings in biological studies. Here, we report a novel 3D approach to acquisition, analysis and interpretation of tumour spheroid images. Our research interest in mesenchymal–amoeboid transition led to the development of a workflow incorporating the generation and analysis of 3D data with instant structured illumination microscopy and a new ImageJ plugin.",

keywords = "Microscopy, 3D, Tumour spheroid, Cell migration, ImageJ",

author = "Sophie Ketchen and Arndt Rohwedder and Sabine Knipp and Filomena Esteves and Nina Struve and Michelle Peckham and John Ladbury and Alistair Curd and Susan Short and Anke Bruning-Richardson",

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T1 - A novel workflow for three-dimensional analysis of tumour cell migration

AU - Ketchen, Sophie

AU - Rohwedder, Arndt

AU - Knipp, Sabine

AU - Esteves, Filomena

AU - Struve, Nina

AU - Peckham, Michelle

AU - Ladbury, John

AU - Curd, Alistair

AU - Short, Susan

AU - Bruning-Richardson, Anke

PY - 2020/4/6

Y1 - 2020/4/6

N2 - The limitations of two-dimensional analysis in three-dimensional (3D) cellular imaging impair the accuracy of research findings in biological studies. Here, we report a novel 3D approach to acquisition, analysis and interpretation of tumour spheroid images. Our research interest in mesenchymal–amoeboid transition led to the development of a workflow incorporating the generation and analysis of 3D data with instant structured illumination microscopy and a new ImageJ plugin.

AB - The limitations of two-dimensional analysis in three-dimensional (3D) cellular imaging impair the accuracy of research findings in biological studies. Here, we report a novel 3D approach to acquisition, analysis and interpretation of tumour spheroid images. Our research interest in mesenchymal–amoeboid transition led to the development of a workflow incorporating the generation and analysis of 3D data with instant structured illumination microscopy and a new ImageJ plugin.

KW - Microscopy

KW - 3D

KW - Tumour spheroid

KW - Cell migration

KW - ImageJ

U2 - 10.1098/rsfs.2019.0070

DO - 10.1098/rsfs.2019.0070

M3 - Article

SN - 2042-8898

VL - 10

JO - Interface Focus

JF - Interface Focus

IS - 2

M1 - 20190070

ER -

A novel workflow for three-dimensional analysis of tumour cell migration

Abstract

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