A prominent β-hairpin structure in the winged-helix domain of RECQ1 is required for DNA unwinding and oligomer formation

Bojana Lucic, Ying Zhang, Oliver King, Ramiro Mendoza-Maldonado, Matteo Berti, Frank H. Niesen, Nicola A. Burgess-Brown, Ashley C W Pike, Christopher D O Cooper, Opher Gileadi, Alessandro Vindigni

Research output: Contribution to journalArticle

40 Citations (Scopus)

Abstract

RecQ helicases have attracted considerable interest in recent years due to their role in the suppression of genome instability and human diseases. These atypical helicases exert their function by resolving a number of highly specific DNA structures. The crystal structure of a truncated catalytic core of the human RECQ1 helicase (RECQ149-616) shows a prominent β-hairpin, with an aromatic residue (Y564) at the tip, located in the C-terminal winged-helix domain. Here, we show that the β-hairpin is required for the DNA unwinding and Holliday junction (HJ) resolution activity of full-length RECQ1, confirming that it represents an important determinant for the distinct substrate specificity of the five human RecQ helicases. In addition, we found that the β-hairpin is required for dimer formation in RECQ1 49-616 and tetramer formation in full-length RECQ1. We confirmed the presence of stable RECQ149-616 dimers in solution and demonstrated that dimer formation favours DNA unwinding; even though RECQ1 monomers are still active. Tetramers are instead necessary for more specialized activities such as HJ resolution and strand annealing. Interestingly, two independent protein-protein contacts are required for tetramer formation, one involves the β-hairpin and the other the N-terminus of RECQ1, suggesting a non-hierarchical mechanism of tetramer assembly.

Original languageEnglish
Pages (from-to)1703-1717
Number of pages15
JournalNucleic Acids Research
Volume39
Issue number5
DOIs
Publication statusPublished - Mar 2011
Externally publishedYes

Fingerprint

RecQ Helicases
Cruciform DNA
DNA
Genomic Instability
Substrate Specificity
Catalytic Domain
Proteins

Cite this

Lucic, B., Zhang, Y., King, O., Mendoza-Maldonado, R., Berti, M., Niesen, F. H., ... Vindigni, A. (2011). A prominent β-hairpin structure in the winged-helix domain of RECQ1 is required for DNA unwinding and oligomer formation. Nucleic Acids Research, 39(5), 1703-1717. https://doi.org/10.1093/nar/gkq1031
Lucic, Bojana ; Zhang, Ying ; King, Oliver ; Mendoza-Maldonado, Ramiro ; Berti, Matteo ; Niesen, Frank H. ; Burgess-Brown, Nicola A. ; Pike, Ashley C W ; Cooper, Christopher D O ; Gileadi, Opher ; Vindigni, Alessandro. / A prominent β-hairpin structure in the winged-helix domain of RECQ1 is required for DNA unwinding and oligomer formation. In: Nucleic Acids Research. 2011 ; Vol. 39, No. 5. pp. 1703-1717.
@article{8c86241521ba4ba59f086f91d3ddfdc6,
title = "A prominent β-hairpin structure in the winged-helix domain of RECQ1 is required for DNA unwinding and oligomer formation",
abstract = "RecQ helicases have attracted considerable interest in recent years due to their role in the suppression of genome instability and human diseases. These atypical helicases exert their function by resolving a number of highly specific DNA structures. The crystal structure of a truncated catalytic core of the human RECQ1 helicase (RECQ149-616) shows a prominent β-hairpin, with an aromatic residue (Y564) at the tip, located in the C-terminal winged-helix domain. Here, we show that the β-hairpin is required for the DNA unwinding and Holliday junction (HJ) resolution activity of full-length RECQ1, confirming that it represents an important determinant for the distinct substrate specificity of the five human RecQ helicases. In addition, we found that the β-hairpin is required for dimer formation in RECQ1 49-616 and tetramer formation in full-length RECQ1. We confirmed the presence of stable RECQ149-616 dimers in solution and demonstrated that dimer formation favours DNA unwinding; even though RECQ1 monomers are still active. Tetramers are instead necessary for more specialized activities such as HJ resolution and strand annealing. Interestingly, two independent protein-protein contacts are required for tetramer formation, one involves the β-hairpin and the other the N-terminus of RECQ1, suggesting a non-hierarchical mechanism of tetramer assembly.",
author = "Bojana Lucic and Ying Zhang and Oliver King and Ramiro Mendoza-Maldonado and Matteo Berti and Niesen, {Frank H.} and Burgess-Brown, {Nicola A.} and Pike, {Ashley C W} and Cooper, {Christopher D O} and Opher Gileadi and Alessandro Vindigni",
year = "2011",
month = "3",
doi = "10.1093/nar/gkq1031",
language = "English",
volume = "39",
pages = "1703--1717",
journal = "Nucleic Acids Research",
issn = "0305-1048",
publisher = "Oxford University Press",
number = "5",

}

Lucic, B, Zhang, Y, King, O, Mendoza-Maldonado, R, Berti, M, Niesen, FH, Burgess-Brown, NA, Pike, ACW, Cooper, CDO, Gileadi, O & Vindigni, A 2011, 'A prominent β-hairpin structure in the winged-helix domain of RECQ1 is required for DNA unwinding and oligomer formation', Nucleic Acids Research, vol. 39, no. 5, pp. 1703-1717. https://doi.org/10.1093/nar/gkq1031

A prominent β-hairpin structure in the winged-helix domain of RECQ1 is required for DNA unwinding and oligomer formation. / Lucic, Bojana; Zhang, Ying; King, Oliver; Mendoza-Maldonado, Ramiro; Berti, Matteo; Niesen, Frank H.; Burgess-Brown, Nicola A.; Pike, Ashley C W; Cooper, Christopher D O; Gileadi, Opher; Vindigni, Alessandro.

In: Nucleic Acids Research, Vol. 39, No. 5, 03.2011, p. 1703-1717.

Research output: Contribution to journalArticle

TY - JOUR

T1 - A prominent β-hairpin structure in the winged-helix domain of RECQ1 is required for DNA unwinding and oligomer formation

AU - Lucic, Bojana

AU - Zhang, Ying

AU - King, Oliver

AU - Mendoza-Maldonado, Ramiro

AU - Berti, Matteo

AU - Niesen, Frank H.

AU - Burgess-Brown, Nicola A.

AU - Pike, Ashley C W

AU - Cooper, Christopher D O

AU - Gileadi, Opher

AU - Vindigni, Alessandro

PY - 2011/3

Y1 - 2011/3

N2 - RecQ helicases have attracted considerable interest in recent years due to their role in the suppression of genome instability and human diseases. These atypical helicases exert their function by resolving a number of highly specific DNA structures. The crystal structure of a truncated catalytic core of the human RECQ1 helicase (RECQ149-616) shows a prominent β-hairpin, with an aromatic residue (Y564) at the tip, located in the C-terminal winged-helix domain. Here, we show that the β-hairpin is required for the DNA unwinding and Holliday junction (HJ) resolution activity of full-length RECQ1, confirming that it represents an important determinant for the distinct substrate specificity of the five human RecQ helicases. In addition, we found that the β-hairpin is required for dimer formation in RECQ1 49-616 and tetramer formation in full-length RECQ1. We confirmed the presence of stable RECQ149-616 dimers in solution and demonstrated that dimer formation favours DNA unwinding; even though RECQ1 monomers are still active. Tetramers are instead necessary for more specialized activities such as HJ resolution and strand annealing. Interestingly, two independent protein-protein contacts are required for tetramer formation, one involves the β-hairpin and the other the N-terminus of RECQ1, suggesting a non-hierarchical mechanism of tetramer assembly.

AB - RecQ helicases have attracted considerable interest in recent years due to their role in the suppression of genome instability and human diseases. These atypical helicases exert their function by resolving a number of highly specific DNA structures. The crystal structure of a truncated catalytic core of the human RECQ1 helicase (RECQ149-616) shows a prominent β-hairpin, with an aromatic residue (Y564) at the tip, located in the C-terminal winged-helix domain. Here, we show that the β-hairpin is required for the DNA unwinding and Holliday junction (HJ) resolution activity of full-length RECQ1, confirming that it represents an important determinant for the distinct substrate specificity of the five human RecQ helicases. In addition, we found that the β-hairpin is required for dimer formation in RECQ1 49-616 and tetramer formation in full-length RECQ1. We confirmed the presence of stable RECQ149-616 dimers in solution and demonstrated that dimer formation favours DNA unwinding; even though RECQ1 monomers are still active. Tetramers are instead necessary for more specialized activities such as HJ resolution and strand annealing. Interestingly, two independent protein-protein contacts are required for tetramer formation, one involves the β-hairpin and the other the N-terminus of RECQ1, suggesting a non-hierarchical mechanism of tetramer assembly.

UR - http://www.scopus.com/inward/record.url?scp=79953158070&partnerID=8YFLogxK

U2 - 10.1093/nar/gkq1031

DO - 10.1093/nar/gkq1031

M3 - Article

VL - 39

SP - 1703

EP - 1717

JO - Nucleic Acids Research

JF - Nucleic Acids Research

SN - 0305-1048

IS - 5

ER -