A rapid chromatographic strip test for the pen-side diagnosis of rinderpest virus

A Brüning, K Bellamy, D Talbot, J Anderson

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

Rinderpest is a contagious viral disease of cloven-hoofed domestic and wild animals. Eradication of the virus following outbreaks depends on rapid and accurate diagnosis of infection and the implementation of control measures. Reporting and confirmatory diagnosis precede the implementation of control measures. A number of techniques have been used for diagnosis such as agar gel immunodiffusion, enzyme-linked immunosorbent assay (ELISA), molecular biological techniques such as polymerase chain reaction (PCR) and virus isolation in tissue culture. Many of these methods are both time consuming and require skilled personnel. The development of a rapid pen-side test for the detection of rinderpest virus (RPV) antigen in lachrymal fluid of cattle is described using the Clearview chromatographic strip test technology (Unipath, Bedford). Optimum conditions for binding monoclonal antibody to nitrocellulose and latex microspheres were determined and a prototype device was developed. The device detected viral antigen in lachrymal fluids from experimentally and naturally infected cattle and showed no cross-reactivity with other related viruses. A field trial was carried out at the Landhi Cattle Colony (LCC), Pakistan, to assess the performance of the rinderpest test under field conditions. Ninety-seven animals, some of which were showing various clinical signs, at LCC and neighbouring colonies were sampled and tested at the pen-side by Clearview and later by immunocapture ELISA (IC-ELISA) at IAH, Pirbright. Nineteen animals were positive by Clearview and/or IC-ELISA. Seventeen out of 19 rinderpest positive animals were positive by Clearview and 15 out of 19 were positive by IC-ELISA. Reverse transcription polymerase chain reaction (RT-PCR) confirmed the 19 animals to be rinderpest positive. This simple, rapid, specific test allows for the first time, accurate pen-side diagnosis of rinderpest.

Original languageEnglish
Pages (from-to)143-54
Number of pages12
JournalJournal of Virological Methods
Volume81
Issue number1-2
DOIs
Publication statusPublished - Aug 1999
Externally publishedYes

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Rinderpest virus
Rinderpest
Enzyme-Linked Immunosorbent Assay
Viruses
Equipment and Supplies
Polymerase Chain Reaction
Wild Animals
Collodion
Viral Antigens
Immunodiffusion
Pakistan
Domestic Animals
Latex
Virus Diseases
Infection Control
Microspheres
Reverse Transcription
Agar
Disease Outbreaks
Gels

Cite this

Brüning, A ; Bellamy, K ; Talbot, D ; Anderson, J. / A rapid chromatographic strip test for the pen-side diagnosis of rinderpest virus. In: Journal of Virological Methods. 1999 ; Vol. 81, No. 1-2. pp. 143-54.
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A rapid chromatographic strip test for the pen-side diagnosis of rinderpest virus. / Brüning, A; Bellamy, K; Talbot, D; Anderson, J.

In: Journal of Virological Methods, Vol. 81, No. 1-2, 08.1999, p. 143-54.

Research output: Contribution to journalArticle

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T1 - A rapid chromatographic strip test for the pen-side diagnosis of rinderpest virus

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AU - Bellamy, K

AU - Talbot, D

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AB - Rinderpest is a contagious viral disease of cloven-hoofed domestic and wild animals. Eradication of the virus following outbreaks depends on rapid and accurate diagnosis of infection and the implementation of control measures. Reporting and confirmatory diagnosis precede the implementation of control measures. A number of techniques have been used for diagnosis such as agar gel immunodiffusion, enzyme-linked immunosorbent assay (ELISA), molecular biological techniques such as polymerase chain reaction (PCR) and virus isolation in tissue culture. Many of these methods are both time consuming and require skilled personnel. The development of a rapid pen-side test for the detection of rinderpest virus (RPV) antigen in lachrymal fluid of cattle is described using the Clearview chromatographic strip test technology (Unipath, Bedford). Optimum conditions for binding monoclonal antibody to nitrocellulose and latex microspheres were determined and a prototype device was developed. The device detected viral antigen in lachrymal fluids from experimentally and naturally infected cattle and showed no cross-reactivity with other related viruses. A field trial was carried out at the Landhi Cattle Colony (LCC), Pakistan, to assess the performance of the rinderpest test under field conditions. Ninety-seven animals, some of which were showing various clinical signs, at LCC and neighbouring colonies were sampled and tested at the pen-side by Clearview and later by immunocapture ELISA (IC-ELISA) at IAH, Pirbright. Nineteen animals were positive by Clearview and/or IC-ELISA. Seventeen out of 19 rinderpest positive animals were positive by Clearview and 15 out of 19 were positive by IC-ELISA. Reverse transcription polymerase chain reaction (RT-PCR) confirmed the 19 animals to be rinderpest positive. This simple, rapid, specific test allows for the first time, accurate pen-side diagnosis of rinderpest.

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KW - Enzyme-Linked Immunosorbent Assay/veterinary

KW - Pakistan

KW - Reagent Kits, Diagnostic/veterinary

KW - Reverse Transcriptase Polymerase Chain Reaction

KW - Rinderpest/diagnosis

KW - Rinderpest virus/immunology

KW - Sensitivity and Specificity

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