Abstract
Industrial production of bioethanol from lignocellulosic materials (LCM′s) is reliant on a microorganism being tolerant to the stresses inherent to fermentation. Previous work has highlighted the importance of a cytochrome oxidase chaperone gene (COX20) in improving yeast tolerance to acetic acid, a common inhibitory compound produced during pre-treatment of LCM’s. The presence of acetic acid has been shown to induce oxidative stress and programmed cell death, so the role of COX20 in oxidative stress was determined. Analysis using flow cytometry revealed that COX20 expression was associated with reduced levels of reactive oxygen species (ROS) in hydrogen peroxide and metal-induced stress, and there was a reduction in apoptotic and necrotic cells when compared with a strain without COX20. Results on the functionality of COX20 have revealed that overexpression of COX20 induced respiratory growth in Δimp1 and Δcox18, two genes whose presence is essential for yeast respiratory growth. COX20 also has a role in protecting the yeast cell against programmed cell death.
| Original language | English |
|---|---|
| Article number | 575 |
| Pages (from-to) | 1-12 |
| Number of pages | 12 |
| Journal | Microorganisms |
| Volume | 7 |
| Issue number | 11 |
| DOIs | |
| Publication status | Published - 18 Nov 2019 |
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A Role for COX20 in Tolerance to Oxidative Stress and Programmed Cell Death in Saccharomyces cerevisiae. / Keerthiraju, Ethiraju; Du, Chenyu; Tucker, Gregory; Greetham, Darren.
In: Microorganisms, Vol. 7, No. 11, 575, 18.11.2019, p. 1-12.Research output: Contribution to journal › Article
TY - JOUR
T1 - A Role for COX20 in Tolerance to Oxidative Stress and Programmed Cell Death in Saccharomyces cerevisiae
AU - Keerthiraju, Ethiraju
AU - Du, Chenyu
AU - Tucker, Gregory
AU - Greetham, Darren
PY - 2019/11/18
Y1 - 2019/11/18
N2 - Industrial production of bioethanol from lignocellulosic materials (LCM′s) is reliant on a microorganism being tolerant to the stresses inherent to fermentation. Previous work has highlighted the importance of a cytochrome oxidase chaperone gene (COX20) in improving yeast tolerance to acetic acid, a common inhibitory compound produced during pre-treatment of LCM’s. The presence of acetic acid has been shown to induce oxidative stress and programmed cell death, so the role of COX20 in oxidative stress was determined. Analysis using flow cytometry revealed that COX20 expression was associated with reduced levels of reactive oxygen species (ROS) in hydrogen peroxide and metal-induced stress, and there was a reduction in apoptotic and necrotic cells when compared with a strain without COX20. Results on the functionality of COX20 have revealed that overexpression of COX20 induced respiratory growth in Δimp1 and Δcox18, two genes whose presence is essential for yeast respiratory growth. COX20 also has a role in protecting the yeast cell against programmed cell death.
AB - Industrial production of bioethanol from lignocellulosic materials (LCM′s) is reliant on a microorganism being tolerant to the stresses inherent to fermentation. Previous work has highlighted the importance of a cytochrome oxidase chaperone gene (COX20) in improving yeast tolerance to acetic acid, a common inhibitory compound produced during pre-treatment of LCM’s. The presence of acetic acid has been shown to induce oxidative stress and programmed cell death, so the role of COX20 in oxidative stress was determined. Analysis using flow cytometry revealed that COX20 expression was associated with reduced levels of reactive oxygen species (ROS) in hydrogen peroxide and metal-induced stress, and there was a reduction in apoptotic and necrotic cells when compared with a strain without COX20. Results on the functionality of COX20 have revealed that overexpression of COX20 induced respiratory growth in Δimp1 and Δcox18, two genes whose presence is essential for yeast respiratory growth. COX20 also has a role in protecting the yeast cell against programmed cell death.
KW - COX20
KW - Oxidative stress
KW - Programmed cell death
KW - Respiratory growth
KW - Yeast
UR - http://www.scopus.com/inward/record.url?scp=85075304358&partnerID=8YFLogxK
U2 - 10.3390/microorganisms7110575
DO - 10.3390/microorganisms7110575
M3 - Article
AN - SCOPUS:85075304358
VL - 7
SP - 1
EP - 12
JO - Microorganisms
JF - Microorganisms
SN - 2076-2607
IS - 11
M1 - 575
ER -