A seawater-based biorefining strategy for fermentative production and chemical transformations of succinic acid

Carol S.K. Lin, Rafael Luque, James H. Clark, Colin Webb, Chenyu Du

Research output: Contribution to journalArticle

44 Citations (Scopus)

Abstract

In this manuscript, the possibility of utilising seawater instead of fresh water and a synthetic mixture of minerals as alternative water and mineral sources in succinic acid (SA) fermentations was investigated. Seawater tolerance experiments demonstrated that the specific growth rate of Actinobacillus succinogenes was only slightly affected when more than 60% synthetic seawater was used, but no major significant inhibition of cell growth and SA production were observed even when fresh water was replaced by 100% synthetic seawater. The possibility of replacing a semi-defined medium with fresh water by wheat-derived media with natural seawater was carried out sequentially. Results showed that besides the usage as a water source, seawater also can be used as a mineral supplement to the wheat-derived media, forming a nutrient-complete medium for succinic acid production. In a fermentation using only wheat-derived medium and natural seawater, 49 g L-1 succinic acid was produced with a yield of 0.94 g per g and a productivity of 1.12 g L-1 h -1. Interestingly, compounds present in seawater had a major effect on rates of reactions of a range of transformations of SA including esterifications and amidations in comparison with reactions run under similar conditions using distilled water. While salts and related compounds improved the rates of reaction in the amidation of SA compared to plain water, a significant reduction in activity and catalyst deactivation was found in the esterifications of SA using Starbon® acids as catalysts.

LanguageEnglish
Pages1471-1479
Number of pages9
JournalEnergy and Environmental Science
Volume4
Issue number4
DOIs
Publication statusPublished - 7 Mar 2011
Externally publishedYes

Fingerprint

Succinic Acid
Seawater
seawater
Acids
acid
Water
Minerals
wheat
water
Esterification
Fermentation
fermentation
mineral
catalyst
Mineral Waters
Catalyst deactivation
chemical
Cell growth
Nutrients
Thermodynamic properties

Cite this

Lin, Carol S.K. ; Luque, Rafael ; Clark, James H. ; Webb, Colin ; Du, Chenyu. / A seawater-based biorefining strategy for fermentative production and chemical transformations of succinic acid. In: Energy and Environmental Science. 2011 ; Vol. 4, No. 4. pp. 1471-1479.
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A seawater-based biorefining strategy for fermentative production and chemical transformations of succinic acid. / Lin, Carol S.K.; Luque, Rafael; Clark, James H.; Webb, Colin; Du, Chenyu.

In: Energy and Environmental Science, Vol. 4, No. 4, 07.03.2011, p. 1471-1479.

Research output: Contribution to journalArticle

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