A structurally self-assembled peptide nano-architecture by one-step electrospinning

Robabeh Gharaei, Giuseppe Tronci, Robert P. Davies, Caroline Gough, Reem Alazragi, Parikshit Goswami, Stephen J. Russell

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Self-assembling peptides (SAPs) have been shown to offer great promise in therapeutics and have the ability to undergo self-assembly and form ordered nanostructures. However SAP gels are often associated with inherent weak and transient mechanical properties and incorporation of them into polymeric matrices is a route to enhance their mechanical stability. The aim of this work was to incorporate P11-8 peptide (CH3COQQRFOWOFEQQNH2) within poly(ε-caprolactone) (PCL) fibrous webs via one-step electrospinning, aiming to establish the underlying relationships between spinning process, molecular peptide conformation, and material internal architecture. Electrospinning of PCL solutions (6% w/w) in hexafluoro-2-propanol (HFIP) containing up to 40 mg mL−1 P11-8 resulted in the formation of fibres in both nano- (10–100 nm) and submicron range (100–700 nm), in contrast to PCL only webs, which displayed a predominantly submicron fibre distribution. FTIR and CD spectroscopy on both PCL/peptide solutions and resulting electrospun webs revealed monomeric and β-sheet secondary conformation, respectively, suggesting the occurrence of peptide self-assembly during electrospinning due to solvent evaporation. The peptide concentration (0 → 40 mg mL−1) was found to primarily affect the internal structure of the fabric at the nano-scale, whilst water as well as cell culture medium contact angles were dramatically decreased. Nearly no cytotoxic response (>90% cell viability) was observed when L929 mouse fibroblasts were cultured in contact with electrospun peptide loaded samples. This novel nanofibrous architecture may be the basis for an interesting material platform for e.g. hard tissue repair, in light of the presence of the self-assembled P11-8 in the PCL fibrous structure.
LanguageEnglish
Pages5475-5485
Number of pages11
JournalJournal of Materials Chemistry B
Volume4
Issue number32
DOIs
Publication statusPublished - 25 Jul 2016
Externally publishedYes

Fingerprint

Electrospinning
Peptides
Self assembly
Conformations
Fibers
Mechanical stability
Propanol
Fibroblasts
Cell culture
Contact angle
Culture Media
Nanostructures
Evaporation
Repair
Gels
Cells
polycaprolactone
Spectroscopy
Tissue
Mechanical properties

Cite this

Gharaei, R., Tronci, G., Davies, R. P., Gough, C., Alazragi, R., Goswami, P., & Russell, S. J. (2016). A structurally self-assembled peptide nano-architecture by one-step electrospinning. Journal of Materials Chemistry B, 4(32), 5475-5485. https://doi.org/10.1039/c6tb01164k
Gharaei, Robabeh ; Tronci, Giuseppe ; Davies, Robert P. ; Gough, Caroline ; Alazragi, Reem ; Goswami, Parikshit ; Russell, Stephen J. / A structurally self-assembled peptide nano-architecture by one-step electrospinning. In: Journal of Materials Chemistry B. 2016 ; Vol. 4, No. 32. pp. 5475-5485.
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Gharaei, R, Tronci, G, Davies, RP, Gough, C, Alazragi, R, Goswami, P & Russell, SJ 2016, 'A structurally self-assembled peptide nano-architecture by one-step electrospinning', Journal of Materials Chemistry B, vol. 4, no. 32, pp. 5475-5485. https://doi.org/10.1039/c6tb01164k

A structurally self-assembled peptide nano-architecture by one-step electrospinning. / Gharaei, Robabeh; Tronci, Giuseppe; Davies, Robert P.; Gough, Caroline; Alazragi, Reem; Goswami, Parikshit; Russell, Stephen J.

In: Journal of Materials Chemistry B, Vol. 4, No. 32, 25.07.2016, p. 5475-5485.

Research output: Contribution to journalArticle

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T1 - A structurally self-assembled peptide nano-architecture by one-step electrospinning

AU - Gharaei, Robabeh

AU - Tronci, Giuseppe

AU - Davies, Robert P.

AU - Gough, Caroline

AU - Alazragi, Reem

AU - Goswami, Parikshit

AU - Russell, Stephen J.

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