Accurate determination of phenotypic information from historic thoroughbred horses by single base extension

Michael G. Campana, C. Mark Whitten, Ceiridwen J. Edwards, Frauke Stock, Angela M. Murphy, Matthew M. Binns, Graeme W.W. Barker, Mim A. Bower

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Historic DNA data have the potential to identify phenotypic information otherwise invisible in the historical, archaeological and palaeontological record. In order to determine whether a single nucleotide polymorphism typing protocol based on single based extension (SNaPshotTM) could produce reliable phenotypic data from historic samples, we genotyped three coat colour markers for a sample of historic Thoroughbred horses for which both phenotypic and correct genotypic information were known from pedigree information in the General Stud Book. Experimental results were consistent with the pedigrees in all cases. Thus we demonstrate that historic DNA techniques can produce reliable phenotypic information from museum specimens.

LanguageEnglish
Article numbere15172
JournalPLoS One
Volume5
Issue number12
DOIs
Publication statusPublished - 15 Dec 2010
Externally publishedYes

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Pedigree
Horses
horses
Museums
DNA
Polymorphism
pedigree
Single Nucleotide Polymorphism
Nucleotides
Color
single nucleotide polymorphism
sampling
color
methodology

Cite this

Campana, M. G., Mark Whitten, C., Edwards, C. J., Stock, F., Murphy, A. M., Binns, M. M., ... Bower, M. A. (2010). Accurate determination of phenotypic information from historic thoroughbred horses by single base extension. PLoS One, 5(12), [e15172]. https://doi.org/10.1371/journal.pone.0015172
Campana, Michael G. ; Mark Whitten, C. ; Edwards, Ceiridwen J. ; Stock, Frauke ; Murphy, Angela M. ; Binns, Matthew M. ; Barker, Graeme W.W. ; Bower, Mim A. / Accurate determination of phenotypic information from historic thoroughbred horses by single base extension. In: PLoS One. 2010 ; Vol. 5, No. 12.
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Campana, MG, Mark Whitten, C, Edwards, CJ, Stock, F, Murphy, AM, Binns, MM, Barker, GWW & Bower, MA 2010, 'Accurate determination of phenotypic information from historic thoroughbred horses by single base extension', PLoS One, vol. 5, no. 12, e15172. https://doi.org/10.1371/journal.pone.0015172

Accurate determination of phenotypic information from historic thoroughbred horses by single base extension. / Campana, Michael G.; Mark Whitten, C.; Edwards, Ceiridwen J.; Stock, Frauke; Murphy, Angela M.; Binns, Matthew M.; Barker, Graeme W.W.; Bower, Mim A.

In: PLoS One, Vol. 5, No. 12, e15172, 15.12.2010.

Research output: Contribution to journalArticle

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