Airyscan Superresolution Microscopy to Study Trypanosomatid Cell Biology

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

The recent introduction by Carl Zeiss Ltd. of the Airyscan detector module for their LSM880 confocal laser-scanning microscope has enabled routine superresolution microscopy to be combined with the advantages of confocal-based fluorescence imaging. Resulting enhanced spatial resolution in X, Y, and Z provides tractable opportunity to derive new insight into protein localization(s), organelle dynamics, and thence protein function within trypanosomatids or other organisms. Here, we describe methods for preparing slides, cells, and basic microscope setup for fluorescence imaging of trypanosomatids using the LSM-880 with Airyscan platform.
Original languageEnglish
Title of host publicationTrypanosomatids
Subtitle of host publicationMethods and Protocols
EditorsPaul A. M. Michels, Michael L. Ginger, Dan Zilberstein
PublisherHumana Press
Chapter27
Pages449-461
Number of pages13
Volume2116
ISBN (Electronic)9781071602942
ISBN (Print)9781071602935
DOIs
Publication statusPublished - 28 Mar 2020

Publication series

NameMethods in Molecular Biology
PublisherHumana Press
Volume2116
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

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  • Cite this

    Harmer, J., Belbelazi, A., Carr, M., & Ginger, M. (2020). Airyscan Superresolution Microscopy to Study Trypanosomatid Cell Biology. In P. A. M. Michels, M. L. Ginger, & D. Zilberstein (Eds.), Trypanosomatids: Methods and Protocols (Vol. 2116, pp. 449-461). (Methods in Molecular Biology; Vol. 2116). Humana Press. https://doi.org/10.1007/978-1-0716-0294-2_27