Alginate hydrogel has a negative impact on in vitro collagen 1 deposition by fibroblasts

Alan M. Smith, Nicola C. Hunt, Richard M. Shelton, Gurpreet Birdi, Liam M. Grover

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Hydrogels have been widely investigated as 3D culture substrates because of their reported structural similarity to the extracellular matrix (ECM). Limited ECM deposition, however, occurs within these materials, so the resulting "tissues" bear little resemblance to those found in the body. Here matrix deposition by fibroblasts encapsulated within a calcium alginate (Ca-alg) hydrogel was investigated. Although the cells transcribed mRNA for coll Iα over a period of 3 weeks, very little collagen protein deposition was observed within the gel by histology or immunohistochemistry (IHC). Although molecular diffusion demonstrated charge dependency, this did not prevent the flux of both positively and negative charged amino acids through the gel, suggesting that the absence of ECM could not be attributed to substrate limitation. The flux of protein, however, was charge-dependent as proteins with a net negative charge passed quickly through the Ca-alg into the medium. The minimal collagen deposition within the Ca-alg was attributed to a combination of rapid movement of negatively charged procollagen through the gel and steric hindrance of fibril formation.

Original languageEnglish
Pages (from-to)4032-4038
Number of pages7
JournalBiomacromolecules
Volume13
Issue number12
Early online date19 Oct 2012
DOIs
Publication statusPublished - 10 Dec 2012

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