Alginate-Loaded Liposomes Can Protect Encapsulated Alkaline Phosphatase Functionality When Exposed to Gastric pH

Alan M. Smith, Monica R. Jaime-Fonseca, Liam M. Grover, Serafim Bakalis

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

The present study has evaluated the potential of alginate-loaded liposomes as a vehicle for the oral delivery of bioactive proteins. The vesicles were prepared from the phospholipid dipalmatoyl phosphatidylcholine using a simple dry film hydration technique. Alkaline phosphatase (ALP) was used as a model bioactive protein, which was encapsulated in alginate-loaded liposomes and conventional liposomes. These vesicles were subject to physical characterization and stability analysis at varying pH values. The enzymatic activity of the ALP was evaluated following exposure to simulated gastric pH. The alginate-loaded liposomes were typically of the order of 10 μm; however, there was evidence of vesicle aggregation thought to be due to alginate present on the surface of the vesicles. The typical size of the aggregated vesicles was ∼30 μm. The enzyme activity of APL following 2 h of exposure to simulated gastric pH was maintained at a significantly higher level when encapsulated in the alginate-loaded liposomes as compared to ALP loaded in conventional liposomes [80% as compared to 55% (p < 0.05), respectively].

LanguageEnglish
Pages4719-4724
Number of pages6
JournalJournal of Agricultural and Food Chemistry
Volume58
Issue number8
DOIs
Publication statusPublished - 28 Apr 2010
Externally publishedYes

Fingerprint

alginates
Liposomes
Alkaline Phosphatase
alkaline phosphatase
Stomach
stomach
films (materials)
phosphatidylcholines
Enzyme activity
mouth
Phosphatidylcholines
phospholipids
Hydration
proteins
alginic acid
enzyme activity
Phospholipids
Proteins
Agglomeration
Enzymes

Cite this

Smith, Alan M. ; Jaime-Fonseca, Monica R. ; Grover, Liam M. ; Bakalis, Serafim. / Alginate-Loaded Liposomes Can Protect Encapsulated Alkaline Phosphatase Functionality When Exposed to Gastric pH. In: Journal of Agricultural and Food Chemistry. 2010 ; Vol. 58, No. 8. pp. 4719-4724.
@article{d882806e048942fda2ea6f3043ebd522,
title = "Alginate-Loaded Liposomes Can Protect Encapsulated Alkaline Phosphatase Functionality When Exposed to Gastric pH",
abstract = "The present study has evaluated the potential of alginate-loaded liposomes as a vehicle for the oral delivery of bioactive proteins. The vesicles were prepared from the phospholipid dipalmatoyl phosphatidylcholine using a simple dry film hydration technique. Alkaline phosphatase (ALP) was used as a model bioactive protein, which was encapsulated in alginate-loaded liposomes and conventional liposomes. These vesicles were subject to physical characterization and stability analysis at varying pH values. The enzymatic activity of the ALP was evaluated following exposure to simulated gastric pH. The alginate-loaded liposomes were typically of the order of 10 μm; however, there was evidence of vesicle aggregation thought to be due to alginate present on the surface of the vesicles. The typical size of the aggregated vesicles was ∼30 μm. The enzyme activity of APL following 2 h of exposure to simulated gastric pH was maintained at a significantly higher level when encapsulated in the alginate-loaded liposomes as compared to ALP loaded in conventional liposomes [80{\%} as compared to 55{\%} (p < 0.05), respectively].",
keywords = "Alginate, Alkaline phosphatase functionality, Gastric pH, Liposomes, Oral",
author = "Smith, {Alan M.} and Jaime-Fonseca, {Monica R.} and Grover, {Liam M.} and Serafim Bakalis",
year = "2010",
month = "4",
day = "28",
doi = "10.1021/jf904466p",
language = "English",
volume = "58",
pages = "4719--4724",
journal = "Journal of Agricultural and Food Chemistry",
issn = "0021-8561",
publisher = "American Chemical Society",
number = "8",

}

Alginate-Loaded Liposomes Can Protect Encapsulated Alkaline Phosphatase Functionality When Exposed to Gastric pH. / Smith, Alan M.; Jaime-Fonseca, Monica R.; Grover, Liam M.; Bakalis, Serafim.

In: Journal of Agricultural and Food Chemistry, Vol. 58, No. 8, 28.04.2010, p. 4719-4724.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Alginate-Loaded Liposomes Can Protect Encapsulated Alkaline Phosphatase Functionality When Exposed to Gastric pH

AU - Smith, Alan M.

AU - Jaime-Fonseca, Monica R.

AU - Grover, Liam M.

AU - Bakalis, Serafim

PY - 2010/4/28

Y1 - 2010/4/28

N2 - The present study has evaluated the potential of alginate-loaded liposomes as a vehicle for the oral delivery of bioactive proteins. The vesicles were prepared from the phospholipid dipalmatoyl phosphatidylcholine using a simple dry film hydration technique. Alkaline phosphatase (ALP) was used as a model bioactive protein, which was encapsulated in alginate-loaded liposomes and conventional liposomes. These vesicles were subject to physical characterization and stability analysis at varying pH values. The enzymatic activity of the ALP was evaluated following exposure to simulated gastric pH. The alginate-loaded liposomes were typically of the order of 10 μm; however, there was evidence of vesicle aggregation thought to be due to alginate present on the surface of the vesicles. The typical size of the aggregated vesicles was ∼30 μm. The enzyme activity of APL following 2 h of exposure to simulated gastric pH was maintained at a significantly higher level when encapsulated in the alginate-loaded liposomes as compared to ALP loaded in conventional liposomes [80% as compared to 55% (p < 0.05), respectively].

AB - The present study has evaluated the potential of alginate-loaded liposomes as a vehicle for the oral delivery of bioactive proteins. The vesicles were prepared from the phospholipid dipalmatoyl phosphatidylcholine using a simple dry film hydration technique. Alkaline phosphatase (ALP) was used as a model bioactive protein, which was encapsulated in alginate-loaded liposomes and conventional liposomes. These vesicles were subject to physical characterization and stability analysis at varying pH values. The enzymatic activity of the ALP was evaluated following exposure to simulated gastric pH. The alginate-loaded liposomes were typically of the order of 10 μm; however, there was evidence of vesicle aggregation thought to be due to alginate present on the surface of the vesicles. The typical size of the aggregated vesicles was ∼30 μm. The enzyme activity of APL following 2 h of exposure to simulated gastric pH was maintained at a significantly higher level when encapsulated in the alginate-loaded liposomes as compared to ALP loaded in conventional liposomes [80% as compared to 55% (p < 0.05), respectively].

KW - Alginate

KW - Alkaline phosphatase functionality

KW - Gastric pH

KW - Liposomes

KW - Oral

UR - http://www.scopus.com/inward/record.url?scp=77951270392&partnerID=8YFLogxK

UR - http://pubs.acs.org/journal/jafcau

U2 - 10.1021/jf904466p

DO - 10.1021/jf904466p

M3 - Article

VL - 58

SP - 4719

EP - 4724

JO - Journal of Agricultural and Food Chemistry

T2 - Journal of Agricultural and Food Chemistry

JF - Journal of Agricultural and Food Chemistry

SN - 0021-8561

IS - 8

ER -