An evaluation of the DRI-ETG EIA method for the determination of ethyl glucuronide concentrations in clinical and post-mortem urine

Sophie C. Turfus, Tu Vo, Nadia Niehaus, Dimitri Gerostamoulos, Jochen Beyer

Research output: Contribution to journalArticle

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Abstract

A commercial enzyme immunoassay for the qualitative and semi-quantitative measurement of ethyl glucuronide (EtG) in urine was evaluated. Post-mortem (n=800), and clinical urine (n=200) samples were assayed using a Hitachi 902 analyzer. The determined concentrations were compared with those obtained using a previously published liquid chromatography-tandem mass spectrometry (LC-MS/MS) method for the quantification of EtG and ethyl sulfate. Using a cut-off of 0.5μg/ml and LC-MS/MS limit of reporting of 0.1μg/ml, there was a sensitivity of 60.8% and a specificity of 100% for clinical samples. For post-mortem samples, sensitivity and specificity were 82.4% and 97.1%, respectively. When reducing the cut-off to 0.1μg/ml, the sensitivity and specificity were 83.3% and 100% for clinical samples whereas for post-mortem samples the sensitivity and specificity were 90.3 % and 88.3 %, respectively. The best trade-offs between sensitivity and specificity for LC-MS/MS limits of reporting of 0.5 and 0.1μg/ml were achieved when using immunoassay cut-offs of 0.3 and 0.092μg/ml, respectively. There was good correlation between quantitative results obtained by both methods but analysis of samples by LC-MS/MS gave higher concentrations than by enzyme immunoassay (EIA), with a statistically significant proportional bias (P<0.0001, Deming regression) for both sample types. The immunoassay is reliable for the qualitative and semi-quantitative presumptive detection of ethyl glucuronide in urine.

LanguageEnglish
Pages439-445
Number of pages7
JournalDrug Testing and Analysis
Volume5
Issue number6
DOIs
Publication statusPublished - 1 Jun 2013
Externally publishedYes

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immunoassay
Immunoenzyme Techniques
urine
Urine
enzyme
Sensitivity and Specificity
Enzymes
Immunoassay
Liquid chromatography
Mass spectrometry
Tandem Mass Spectrometry
Liquid Chromatography
liquid chromatography
mass spectrometry
sulfate
evaluation
method
ethyl glucuronide

Cite this

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title = "An evaluation of the DRI-ETG EIA method for the determination of ethyl glucuronide concentrations in clinical and post-mortem urine",
abstract = "A commercial enzyme immunoassay for the qualitative and semi-quantitative measurement of ethyl glucuronide (EtG) in urine was evaluated. Post-mortem (n=800), and clinical urine (n=200) samples were assayed using a Hitachi 902 analyzer. The determined concentrations were compared with those obtained using a previously published liquid chromatography-tandem mass spectrometry (LC-MS/MS) method for the quantification of EtG and ethyl sulfate. Using a cut-off of 0.5μg/ml and LC-MS/MS limit of reporting of 0.1μg/ml, there was a sensitivity of 60.8{\%} and a specificity of 100{\%} for clinical samples. For post-mortem samples, sensitivity and specificity were 82.4{\%} and 97.1{\%}, respectively. When reducing the cut-off to 0.1μg/ml, the sensitivity and specificity were 83.3{\%} and 100{\%} for clinical samples whereas for post-mortem samples the sensitivity and specificity were 90.3 {\%} and 88.3 {\%}, respectively. The best trade-offs between sensitivity and specificity for LC-MS/MS limits of reporting of 0.5 and 0.1μg/ml were achieved when using immunoassay cut-offs of 0.3 and 0.092μg/ml, respectively. There was good correlation between quantitative results obtained by both methods but analysis of samples by LC-MS/MS gave higher concentrations than by enzyme immunoassay (EIA), with a statistically significant proportional bias (P<0.0001, Deming regression) for both sample types. The immunoassay is reliable for the qualitative and semi-quantitative presumptive detection of ethyl glucuronide in urine.",
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An evaluation of the DRI-ETG EIA method for the determination of ethyl glucuronide concentrations in clinical and post-mortem urine. / Turfus, Sophie C.; Vo, Tu; Niehaus, Nadia; Gerostamoulos, Dimitri; Beyer, Jochen.

In: Drug Testing and Analysis, Vol. 5, No. 6, 01.06.2013, p. 439-445.

Research output: Contribution to journalArticle

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T1 - An evaluation of the DRI-ETG EIA method for the determination of ethyl glucuronide concentrations in clinical and post-mortem urine

AU - Turfus, Sophie C.

AU - Vo, Tu

AU - Niehaus, Nadia

AU - Gerostamoulos, Dimitri

AU - Beyer, Jochen

PY - 2013/6/1

Y1 - 2013/6/1

N2 - A commercial enzyme immunoassay for the qualitative and semi-quantitative measurement of ethyl glucuronide (EtG) in urine was evaluated. Post-mortem (n=800), and clinical urine (n=200) samples were assayed using a Hitachi 902 analyzer. The determined concentrations were compared with those obtained using a previously published liquid chromatography-tandem mass spectrometry (LC-MS/MS) method for the quantification of EtG and ethyl sulfate. Using a cut-off of 0.5μg/ml and LC-MS/MS limit of reporting of 0.1μg/ml, there was a sensitivity of 60.8% and a specificity of 100% for clinical samples. For post-mortem samples, sensitivity and specificity were 82.4% and 97.1%, respectively. When reducing the cut-off to 0.1μg/ml, the sensitivity and specificity were 83.3% and 100% for clinical samples whereas for post-mortem samples the sensitivity and specificity were 90.3 % and 88.3 %, respectively. The best trade-offs between sensitivity and specificity for LC-MS/MS limits of reporting of 0.5 and 0.1μg/ml were achieved when using immunoassay cut-offs of 0.3 and 0.092μg/ml, respectively. There was good correlation between quantitative results obtained by both methods but analysis of samples by LC-MS/MS gave higher concentrations than by enzyme immunoassay (EIA), with a statistically significant proportional bias (P<0.0001, Deming regression) for both sample types. The immunoassay is reliable for the qualitative and semi-quantitative presumptive detection of ethyl glucuronide in urine.

AB - A commercial enzyme immunoassay for the qualitative and semi-quantitative measurement of ethyl glucuronide (EtG) in urine was evaluated. Post-mortem (n=800), and clinical urine (n=200) samples were assayed using a Hitachi 902 analyzer. The determined concentrations were compared with those obtained using a previously published liquid chromatography-tandem mass spectrometry (LC-MS/MS) method for the quantification of EtG and ethyl sulfate. Using a cut-off of 0.5μg/ml and LC-MS/MS limit of reporting of 0.1μg/ml, there was a sensitivity of 60.8% and a specificity of 100% for clinical samples. For post-mortem samples, sensitivity and specificity were 82.4% and 97.1%, respectively. When reducing the cut-off to 0.1μg/ml, the sensitivity and specificity were 83.3% and 100% for clinical samples whereas for post-mortem samples the sensitivity and specificity were 90.3 % and 88.3 %, respectively. The best trade-offs between sensitivity and specificity for LC-MS/MS limits of reporting of 0.5 and 0.1μg/ml were achieved when using immunoassay cut-offs of 0.3 and 0.092μg/ml, respectively. There was good correlation between quantitative results obtained by both methods but analysis of samples by LC-MS/MS gave higher concentrations than by enzyme immunoassay (EIA), with a statistically significant proportional bias (P<0.0001, Deming regression) for both sample types. The immunoassay is reliable for the qualitative and semi-quantitative presumptive detection of ethyl glucuronide in urine.

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