An evolutionary ratchet leading to loss of elongation factors in eukaryotes

Gemma C. Atkinson, Anton Kuzmenko, Ivan Chicherin, Axel Soosaar, Tanel Tenson, Martin Carr, Piotr Kamenski, Vasili Hauryliuk

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Background: The GTPase eEF1A is the eukaryotic factor responsible for the essential, universal function of aminoacyl-tRNA delivery to the ribosome. Surprisingly, eEF1A is not universally present in eukaryotes, being replaced by the paralog EFL independently in multiple lineages. The driving force behind this unusually frequent replacement is poorly understood. Results: Through sequence searching of genomic and EST databases, we find a striking association of eEF1A replacement by EFL and loss of eEF1A's guanine exchange factor, eEF1Bα, suggesting that EFL is able to spontaneously recharge with GTP. Sequence conservation and homology modeling analyses indicate several sequence regions that may be responsible for EFL's lack of requirement for eEF1Bα. Conclusions: We propose that the unusual pattern of eEF1A, eEF1Bα and EFL presence and absence can be explained by a ratchet-like process: if either eEF1A or eEF1Bα diverges beyond functionality in the presence of EFL, the system is unable to return to the ancestral, eEF1A:eEFBα-driven state.

LanguageEnglish
Article number35
JournalBMC Evolutionary Biology
Volume14
Issue number1
DOIs
Publication statusPublished - 24 Feb 2014

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guanine
guanosinetriphosphatase
eukaryote
ribosomes
eukaryotic cells
replacement
genomics
homology
recharge
modeling
aminoacyl transfer RNA
loss

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Atkinson, G. C., Kuzmenko, A., Chicherin, I., Soosaar, A., Tenson, T., Carr, M., ... Hauryliuk, V. (2014). An evolutionary ratchet leading to loss of elongation factors in eukaryotes. BMC Evolutionary Biology, 14(1), [35]. https://doi.org/10.1186/1471-2148-14-35
Atkinson, Gemma C. ; Kuzmenko, Anton ; Chicherin, Ivan ; Soosaar, Axel ; Tenson, Tanel ; Carr, Martin ; Kamenski, Piotr ; Hauryliuk, Vasili. / An evolutionary ratchet leading to loss of elongation factors in eukaryotes. In: BMC Evolutionary Biology. 2014 ; Vol. 14, No. 1.
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Atkinson, GC, Kuzmenko, A, Chicherin, I, Soosaar, A, Tenson, T, Carr, M, Kamenski, P & Hauryliuk, V 2014, 'An evolutionary ratchet leading to loss of elongation factors in eukaryotes', BMC Evolutionary Biology, vol. 14, no. 1, 35. https://doi.org/10.1186/1471-2148-14-35

An evolutionary ratchet leading to loss of elongation factors in eukaryotes. / Atkinson, Gemma C.; Kuzmenko, Anton; Chicherin, Ivan; Soosaar, Axel; Tenson, Tanel; Carr, Martin; Kamenski, Piotr; Hauryliuk, Vasili.

In: BMC Evolutionary Biology, Vol. 14, No. 1, 35, 24.02.2014.

Research output: Contribution to journalArticle

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T1 - An evolutionary ratchet leading to loss of elongation factors in eukaryotes

AU - Atkinson, Gemma C.

AU - Kuzmenko, Anton

AU - Chicherin, Ivan

AU - Soosaar, Axel

AU - Tenson, Tanel

AU - Carr, Martin

AU - Kamenski, Piotr

AU - Hauryliuk, Vasili

PY - 2014/2/24

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N2 - Background: The GTPase eEF1A is the eukaryotic factor responsible for the essential, universal function of aminoacyl-tRNA delivery to the ribosome. Surprisingly, eEF1A is not universally present in eukaryotes, being replaced by the paralog EFL independently in multiple lineages. The driving force behind this unusually frequent replacement is poorly understood. Results: Through sequence searching of genomic and EST databases, we find a striking association of eEF1A replacement by EFL and loss of eEF1A's guanine exchange factor, eEF1Bα, suggesting that EFL is able to spontaneously recharge with GTP. Sequence conservation and homology modeling analyses indicate several sequence regions that may be responsible for EFL's lack of requirement for eEF1Bα. Conclusions: We propose that the unusual pattern of eEF1A, eEF1Bα and EFL presence and absence can be explained by a ratchet-like process: if either eEF1A or eEF1Bα diverges beyond functionality in the presence of EFL, the system is unable to return to the ancestral, eEF1A:eEFBα-driven state.

AB - Background: The GTPase eEF1A is the eukaryotic factor responsible for the essential, universal function of aminoacyl-tRNA delivery to the ribosome. Surprisingly, eEF1A is not universally present in eukaryotes, being replaced by the paralog EFL independently in multiple lineages. The driving force behind this unusually frequent replacement is poorly understood. Results: Through sequence searching of genomic and EST databases, we find a striking association of eEF1A replacement by EFL and loss of eEF1A's guanine exchange factor, eEF1Bα, suggesting that EFL is able to spontaneously recharge with GTP. Sequence conservation and homology modeling analyses indicate several sequence regions that may be responsible for EFL's lack of requirement for eEF1Bα. Conclusions: We propose that the unusual pattern of eEF1A, eEF1Bα and EFL presence and absence can be explained by a ratchet-like process: if either eEF1A or eEF1Bα diverges beyond functionality in the presence of EFL, the system is unable to return to the ancestral, eEF1A:eEFBα-driven state.

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