An initial evaluation of gellan gum as a material for tissue engineering applications

Alan M. Smith, Richard M. Shelton, Yvonne Perrie, Jonathan J. Harris

Research output: Contribution to journalArticle

106 Citations (Scopus)

Abstract

Alpha-modified minimum essential medium (αMEM) has been found to cross-link a 1% gellan gum solution, resulting in the formation of a self-supporting hydrogel in 1:1 and 5:1 ratios of polysaccharide: αMEM. Rheological data from temperature sweeps confirm that in addition to orders of magnitude differences in G' between 1% gellan and 1% gellan with αMEM, there is also a 20°C increase in the temperature at which the onset of gelation takes place when αMEM is present. Frequency sweeps confirm the formation of a true gel; mechanical spectra for mixtures of gellan and αMEM clearly demonstrate G' to be independent of frequency. It is possible to immobilize cells within a three-dimensional (3D) gellan matrix that remain viable for up to 21 days in culture by adding a suspension of rat bone marrow cells (rBMC) in αMEM to 1% gellan solution. This extremely simple approach to cell immobilization within 3D constructs, made possible by the fact that gellan solutions cross-link in the presence of millimolar concentrations of cations, poses a very low risk to a cell population immobilized within a gellan matrix and thus indicates the potential of gellan for use as a tissue engineering scaffold.

LanguageEnglish
Pages241-254
Number of pages14
JournalJournal of Biomaterials Applications
Volume22
Issue number3
DOIs
Publication statusPublished - Nov 2007
Externally publishedYes

Fingerprint

Tissue engineering
Cell immobilization
Cells
Bioelectric potentials
Gelation
Scaffolds (biology)
Polysaccharides
Hydrogels
Rats
Bone
Gels
Positive ions
Temperature
Tissue Scaffolds
gellan gum
Hydrogel
Cations
Suspensions

Cite this

Smith, Alan M. ; Shelton, Richard M. ; Perrie, Yvonne ; Harris, Jonathan J. / An initial evaluation of gellan gum as a material for tissue engineering applications. In: Journal of Biomaterials Applications. 2007 ; Vol. 22, No. 3. pp. 241-254.
@article{e1bffc1032dd475f9dc26ebb77a372be,
title = "An initial evaluation of gellan gum as a material for tissue engineering applications",
abstract = "Alpha-modified minimum essential medium (αMEM) has been found to cross-link a 1{\%} gellan gum solution, resulting in the formation of a self-supporting hydrogel in 1:1 and 5:1 ratios of polysaccharide: αMEM. Rheological data from temperature sweeps confirm that in addition to orders of magnitude differences in G' between 1{\%} gellan and 1{\%} gellan with αMEM, there is also a 20°C increase in the temperature at which the onset of gelation takes place when αMEM is present. Frequency sweeps confirm the formation of a true gel; mechanical spectra for mixtures of gellan and αMEM clearly demonstrate G' to be independent of frequency. It is possible to immobilize cells within a three-dimensional (3D) gellan matrix that remain viable for up to 21 days in culture by adding a suspension of rat bone marrow cells (rBMC) in αMEM to 1{\%} gellan solution. This extremely simple approach to cell immobilization within 3D constructs, made possible by the fact that gellan solutions cross-link in the presence of millimolar concentrations of cations, poses a very low risk to a cell population immobilized within a gellan matrix and thus indicates the potential of gellan for use as a tissue engineering scaffold.",
keywords = "Bone, Cell viability, Gellan, Hydrogel, Three-dimensional",
author = "Smith, {Alan M.} and Shelton, {Richard M.} and Yvonne Perrie and Harris, {Jonathan J.}",
year = "2007",
month = "11",
doi = "10.1177/0885328207076522",
language = "English",
volume = "22",
pages = "241--254",
journal = "Journal of Biomaterials Applications",
issn = "0885-3282",
publisher = "SAGE Publications Ltd",
number = "3",

}

An initial evaluation of gellan gum as a material for tissue engineering applications. / Smith, Alan M.; Shelton, Richard M.; Perrie, Yvonne; Harris, Jonathan J.

In: Journal of Biomaterials Applications, Vol. 22, No. 3, 11.2007, p. 241-254.

Research output: Contribution to journalArticle

TY - JOUR

T1 - An initial evaluation of gellan gum as a material for tissue engineering applications

AU - Smith, Alan M.

AU - Shelton, Richard M.

AU - Perrie, Yvonne

AU - Harris, Jonathan J.

PY - 2007/11

Y1 - 2007/11

N2 - Alpha-modified minimum essential medium (αMEM) has been found to cross-link a 1% gellan gum solution, resulting in the formation of a self-supporting hydrogel in 1:1 and 5:1 ratios of polysaccharide: αMEM. Rheological data from temperature sweeps confirm that in addition to orders of magnitude differences in G' between 1% gellan and 1% gellan with αMEM, there is also a 20°C increase in the temperature at which the onset of gelation takes place when αMEM is present. Frequency sweeps confirm the formation of a true gel; mechanical spectra for mixtures of gellan and αMEM clearly demonstrate G' to be independent of frequency. It is possible to immobilize cells within a three-dimensional (3D) gellan matrix that remain viable for up to 21 days in culture by adding a suspension of rat bone marrow cells (rBMC) in αMEM to 1% gellan solution. This extremely simple approach to cell immobilization within 3D constructs, made possible by the fact that gellan solutions cross-link in the presence of millimolar concentrations of cations, poses a very low risk to a cell population immobilized within a gellan matrix and thus indicates the potential of gellan for use as a tissue engineering scaffold.

AB - Alpha-modified minimum essential medium (αMEM) has been found to cross-link a 1% gellan gum solution, resulting in the formation of a self-supporting hydrogel in 1:1 and 5:1 ratios of polysaccharide: αMEM. Rheological data from temperature sweeps confirm that in addition to orders of magnitude differences in G' between 1% gellan and 1% gellan with αMEM, there is also a 20°C increase in the temperature at which the onset of gelation takes place when αMEM is present. Frequency sweeps confirm the formation of a true gel; mechanical spectra for mixtures of gellan and αMEM clearly demonstrate G' to be independent of frequency. It is possible to immobilize cells within a three-dimensional (3D) gellan matrix that remain viable for up to 21 days in culture by adding a suspension of rat bone marrow cells (rBMC) in αMEM to 1% gellan solution. This extremely simple approach to cell immobilization within 3D constructs, made possible by the fact that gellan solutions cross-link in the presence of millimolar concentrations of cations, poses a very low risk to a cell population immobilized within a gellan matrix and thus indicates the potential of gellan for use as a tissue engineering scaffold.

KW - Bone

KW - Cell viability

KW - Gellan

KW - Hydrogel

KW - Three-dimensional

UR - http://www.scopus.com/inward/record.url?scp=34648849748&partnerID=8YFLogxK

UR - https://us.sagepub.com/en-us/nam/journal/journal-biomaterials-applications

U2 - 10.1177/0885328207076522

DO - 10.1177/0885328207076522

M3 - Article

VL - 22

SP - 241

EP - 254

JO - Journal of Biomaterials Applications

T2 - Journal of Biomaterials Applications

JF - Journal of Biomaterials Applications

SN - 0885-3282

IS - 3

ER -