TY - JOUR
T1 - Anti-inflammatory action of a taurine analogue, ethane-β-sultam, in phagocytic cells in vivo and in vitro.
AU - Ward, Roberta
AU - Lallemand, Frederic
AU - De Witte, Philippe
AU - Crichton, Robert R.
AU - Piette, Jacques
AU - Tipton, Keith
AU - Hemmings, Karl
AU - Pitard, Arnaud
AU - Page, Michael
AU - Della Corte, Laura
AU - Taylor, Deanna
AU - Dexter, David
PY - 2011/3/15
Y1 - 2011/3/15
N2 - The ability of a taurine prodrug, ethane β-sultam, to reduce cellular inflammation has been investigated, in vitro, in primary cultures of alveolar macrophages and an immortilised N9 microglial cell line and in vivo in an animal model of inflammation and control rats. Ethane β-sultam showed enhanced ability to reduce the inflammatory response in alveolar macrophages, as assayed by the lipopolysaccharide-stimulated–nitric oxide release, (LPS stimulated-NO), in comparison to taurine both in vitro (10 nM, 50 nM) and in vivo (0.15 mmol/kg/day by gavage). In addition, ethane β-sultam, (50, 100 and 1000 nM) significantly reduced LPS-stimulated glutamate release from N9 microglial cells to a greater extent than taurine. The anti-inflammatory response of taurine was shown to be mediated via stabilisation of IkBα. The use of a taurine prodrug as therapeutic agents, for the treatment of neurological conditions, such as Parkinson's and Alzheimer's disease and alcoholic brain damage, where activated phagocytic cells contribute to the pathogenesis, may be of great potential.
AB - The ability of a taurine prodrug, ethane β-sultam, to reduce cellular inflammation has been investigated, in vitro, in primary cultures of alveolar macrophages and an immortilised N9 microglial cell line and in vivo in an animal model of inflammation and control rats. Ethane β-sultam showed enhanced ability to reduce the inflammatory response in alveolar macrophages, as assayed by the lipopolysaccharide-stimulated–nitric oxide release, (LPS stimulated-NO), in comparison to taurine both in vitro (10 nM, 50 nM) and in vivo (0.15 mmol/kg/day by gavage). In addition, ethane β-sultam, (50, 100 and 1000 nM) significantly reduced LPS-stimulated glutamate release from N9 microglial cells to a greater extent than taurine. The anti-inflammatory response of taurine was shown to be mediated via stabilisation of IkBα. The use of a taurine prodrug as therapeutic agents, for the treatment of neurological conditions, such as Parkinson's and Alzheimer's disease and alcoholic brain damage, where activated phagocytic cells contribute to the pathogenesis, may be of great potential.
KW - Taurine
KW - Anti-inflammatory action
KW - Ethane-β sultam
KW - Nitric oxide
KW - Glutamate
UR - https://www.journals.elsevier.com/biochemical-pharmacology
U2 - 10.1016/j.bcp.2010.12.030
DO - 10.1016/j.bcp.2010.12.030
M3 - Article
VL - 81
SP - 743
EP - 751
JO - Biochemical Pharmacology
JF - Biochemical Pharmacology
SN - 0006-2952
IS - 6
ER -