Antimicrobial activity and skin permeation of iodine present in an iodine-impregnated surgical incise drape

A. L. Casey, T. J. Karpanen, P. Nightingale, B. R. Conway, T. S J Elliott

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Objectives: The antimicrobial efficacy of an iodine-impregnated incise drape against MRSA was evaluated in a skin model. The permeation of iodine from this drape into the skin was also assessed. Methods: The antimicrobial efficacy was evaluated in ex vivo studies following application of the surgical incise drape for various times on the surface of donor skin, which was inoculated with either 1 × 103 or 1 × 106 cfu MRSA/cm. 2 skin and mounted on Franz diffusion cells. In some experiments the MRSA-inoculated skin was pre-incubated for 18 h at room temperature prior to applying the drape. Permeation of iodine into the skin using this model was also determined following application of the incise drape for 6 h. Results: The iodine-impregnated drape demonstrated antimicrobial activity compared with the non-use of drape. This reached significance when a high inoculum of MRSA was applied with no pre-incubation period and when a low inoculum of MRSA was applied with a pre-incubation period (P = 0.002 and P = 0.014, respectively). Furthermore, in experiments wherein a high inoculum of MRSA was applied with no pre-incubation period, the iodine-impregnated drape demonstrated superior antimicrobial activity compared with the use of a non-antimicrobial drape (P < 0.001). MIC and MBC values of iodine were attained to 1500 μm below the skin surface. Conclusions: The iodine-impregnated surgical incise drape had detectable antimicrobial activity. Furthermore, iodine penetrated into the deeper layers of the skin. This property should suppress microbial regrowth at and around a surgical incision site, making its use preferable to the use of a standard drape or non-use of a drape.

Original languageEnglish
Article numberdkv100
Pages (from-to)2255-2260
Number of pages6
JournalJournal of Antimicrobial Chemotherapy
Volume70
Issue number8
DOIs
Publication statusPublished - 1 Aug 2015

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