Antimicrobial efficacy of a novel eucalyptus oil, chlorhexidine digluconate and isopropyl alcohol biocide formulation

Emma Hendry, Barbara Conway, Tony Worthington

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Effective surface disinfection is a fundamental infection control strategy within healthcare. This study assessed the antimicrobial efficacy of novel biocide formulations comprising 5% and 2% eucalyptus oil (EO) combined with 2% chlorhexidine digluconate (CHG) and 70% isopropyl alcohol (IPA) contained within a wipe. The efficacy of this novel antimicrobial formulation to remove and eliminate methicillin-resistant Staphylococcus aureus (MRSA), Escherichia coli and Candida albicans from steel surfaces was investigated. Adpression studies of pre-contaminated wipes were also utilised to assess their potential to induce cross-contamination between hard surfaces. Furthermore, the bactericidal nature of the EO-formulation was established in addition to time-kill. The EO-containing formulations demonstrated bactericidal antimicrobial efficacy against all microorganisms and did not induce surface cross-contamination. There was no significant difference (p < 0.05) between the 5% and 2% EO formulations in their ability to remove microorganisms from steel surfaces, however both significantly (p < 0.05) removed more than the control formulations. Microbial biofilms were eliminated within 10 min (p < 0.05) when exposed to the EO formulations. Our novel EO-formulation demonstrated rapid antimicrobial efficacy for potential disinfection and elimination of microbial biofilms from hard surfaces and may therefore be a useful adjunct to current infection control strategies currently employed within healthcare facilities.

LanguageEnglish
Pages14016-14025
Number of pages10
JournalInternational Journal of Molecular Sciences
Volume13
Issue number11
DOIs
Publication statusPublished - 2012

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isopropyl alcohol
Eucalyptus
Biocides
2-Propanol
Disinfectants
Oils
alcohols
Alcohols
oils
formulations
Disinfection
Steel
Biofilms
Infection Control
Microorganisms
biofilms
Contamination
infectious diseases
microorganisms
Delivery of Health Care

Cite this

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title = "Antimicrobial efficacy of a novel eucalyptus oil, chlorhexidine digluconate and isopropyl alcohol biocide formulation",
abstract = "Effective surface disinfection is a fundamental infection control strategy within healthcare. This study assessed the antimicrobial efficacy of novel biocide formulations comprising 5{\%} and 2{\%} eucalyptus oil (EO) combined with 2{\%} chlorhexidine digluconate (CHG) and 70{\%} isopropyl alcohol (IPA) contained within a wipe. The efficacy of this novel antimicrobial formulation to remove and eliminate methicillin-resistant Staphylococcus aureus (MRSA), Escherichia coli and Candida albicans from steel surfaces was investigated. Adpression studies of pre-contaminated wipes were also utilised to assess their potential to induce cross-contamination between hard surfaces. Furthermore, the bactericidal nature of the EO-formulation was established in addition to time-kill. The EO-containing formulations demonstrated bactericidal antimicrobial efficacy against all microorganisms and did not induce surface cross-contamination. There was no significant difference (p < 0.05) between the 5{\%} and 2{\%} EO formulations in their ability to remove microorganisms from steel surfaces, however both significantly (p < 0.05) removed more than the control formulations. Microbial biofilms were eliminated within 10 min (p < 0.05) when exposed to the EO formulations. Our novel EO-formulation demonstrated rapid antimicrobial efficacy for potential disinfection and elimination of microbial biofilms from hard surfaces and may therefore be a useful adjunct to current infection control strategies currently employed within healthcare facilities.",
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Antimicrobial efficacy of a novel eucalyptus oil, chlorhexidine digluconate and isopropyl alcohol biocide formulation. / Hendry, Emma; Conway, Barbara; Worthington, Tony.

In: International Journal of Molecular Sciences, Vol. 13, No. 11, 2012, p. 14016-14025.

Research output: Contribution to journalArticle

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AU - Conway, Barbara

AU - Worthington, Tony

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