Abstract
| Original language | English |
|---|---|
| Article number | ETLS-2018-0015CR1 |
| Pages (from-to) | 503-516 |
| Number of pages | 14 |
| Journal | Emerging Topics in Life Sciences |
| Volume | 2 |
| Issue number | 4 |
| Early online date | 14 Nov 2018 |
| DOIs | |
| Publication status | Published - Dec 2018 |
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Archaeal DNA polymerases : new frontiers in DNA replication and repair. / Cooper, Christopher.
In: Emerging Topics in Life Sciences , Vol. 2, No. 4, ETLS-2018-0015CR1, 12.2018, p. 503-516.Research output: Contribution to journal › Review article
TY - JOUR
T1 - Archaeal DNA polymerases
T2 - new frontiers in DNA replication and repair
AU - Cooper, Christopher
PY - 2018/12
Y1 - 2018/12
N2 - Archaeal DNA polymerases have long been studied due to their superior properties for DNA amplification in the Polymerase Chain Reaction and DNA sequencing technologies. However a full comprehension of their functions, recruitment and regulation as part of the replisome during genome replication and DNA repair lags behind well-established bacterial and eukaryotic model systems. The archaea are evolutionarily very broad, but a number of studies in the major model systems of both Crenarchaeota and Euryarchaeota are starting to yield significant increases in understanding of the functions of DNA polymerases in the respective phyla. Recent advances in biochemical approaches and in archaeal genetic models allowing knockout and epitope tagging have led to significant increases in our understanding, including DNA polymerase roles in Okazaki fragment maturation on the lagging strand, towards reconstitution of the replisome itself. Furthermore, poorly characterised DNA polymerase paralogues are finding roles in DNA repair and CRISPR immunity. This review attempts to provide a current update on the roles of archaeal DNA polymerases in both DNA replication and repair, addressing significant questions that remain for this field.
AB - Archaeal DNA polymerases have long been studied due to their superior properties for DNA amplification in the Polymerase Chain Reaction and DNA sequencing technologies. However a full comprehension of their functions, recruitment and regulation as part of the replisome during genome replication and DNA repair lags behind well-established bacterial and eukaryotic model systems. The archaea are evolutionarily very broad, but a number of studies in the major model systems of both Crenarchaeota and Euryarchaeota are starting to yield significant increases in understanding of the functions of DNA polymerases in the respective phyla. Recent advances in biochemical approaches and in archaeal genetic models allowing knockout and epitope tagging have led to significant increases in our understanding, including DNA polymerase roles in Okazaki fragment maturation on the lagging strand, towards reconstitution of the replisome itself. Furthermore, poorly characterised DNA polymerase paralogues are finding roles in DNA repair and CRISPR immunity. This review attempts to provide a current update on the roles of archaeal DNA polymerases in both DNA replication and repair, addressing significant questions that remain for this field.
KW - Archaea
KW - DNA polymerase
KW - exonuclease
KW - DNA replication
KW - DNA repair
KW - replisome
KW - holoenzyme
KW - sequencing
KW - biotechnology
KW - polB1
KW - Dpo1
KW - PolB
KW - PolD
KW - PCNA
KW - RFC
U2 - 10.1042/ETLS20180015
DO - 10.1042/ETLS20180015
M3 - Review article
VL - 2
SP - 503
EP - 516
JO - Emerging Topics in Life Sciences
JF - Emerging Topics in Life Sciences
SN - 2397-8562
IS - 4
M1 - ETLS-2018-0015CR1
ER -