Archaeal DNA polymerases: new frontiers in DNA replication and repair

Research output: Contribution to journalReview article

Abstract

Archaeal DNA polymerases have long been studied due to their superior properties for DNA amplification in the Polymerase Chain Reaction and DNA sequencing technologies. However a full comprehension of their functions, recruitment and regulation as part of the replisome during genome replication and DNA repair lags behind well-established bacterial and eukaryotic model systems. The archaea are evolutionarily very broad, but a number of studies in the major model systems of both Crenarchaeota and Euryarchaeota are starting to yield significant increases in understanding of the functions of DNA polymerases in the respective phyla. Recent advances in biochemical approaches and in archaeal genetic models allowing knockout and epitope tagging have led to significant increases in our understanding, including DNA polymerase roles in Okazaki fragment maturation on the lagging strand, towards reconstitution of the replisome itself. Furthermore, poorly characterised DNA polymerase paralogues are finding roles in DNA repair and CRISPR immunity. This review attempts to provide a current update on the roles of archaeal DNA polymerases in both DNA replication and repair, addressing significant questions that remain for this field.
Original languageEnglish
Article numberETLS-2018-0015CR1
Pages (from-to)503-516
Number of pages14
JournalEmerging Topics in Life Sciences
Volume2
Issue number4
Early online date14 Nov 2018
DOIs
Publication statusPublished - Dec 2018

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