Aspects of the analytical ultracentrifuge determination of the molar mass distribution of polysaccharides

Stephen E. Harding, Gary G. Adams, Richard B. Gillis, Fahad M. Almutairi, Gordon A. Morris

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Molar mass or ‘molecular weight’ is one of the most fundamental parameters describing a macromolecule. Because of their polydisperse nature, polysaccharides are usually described by distributions of molar mass. SEC-MALS (size exclusion chromatography coupled to multi-angle light scattering) is often a convenient method of choice, but there are many instances where it is unsuitable. Modern AUC (analytical ultracentrifuge) methods provide a valuable alternative - now easier to use than before - and, after briefly reviewing some older procedures, we highlight two recently published and complementary methods, namely, the ‘Extended Fujita’ approach for the analysis of sedimentation velocity data and SEDFIT-MSTAR for the analysis of sedimentation equilibrium data. Nonideality needs to be considered and can be dealt with in a standard way. These methods can also indicate if associative phenomena are present, which can then be quantified using more complex AUC algorithms.

Original languageEnglish
Title of host publicationAnalytical Ultracentrifugation: Instrumentation, Software, and Applications
PublisherSpringer Japan
Pages375-386
Number of pages12
ISBN (Electronic)9784431559856
ISBN (Print)9784431559832
DOIs
Publication statusPublished - 1 Jan 2016

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Molar mass
Polysaccharides
Sedimentation
Size exclusion chromatography
Macromolecules
Light scattering
Molecular weight
Gel Chromatography
Molecular Weight
Light

Cite this

Harding, S. E., Adams, G. G., Gillis, R. B., Almutairi, F. M., & Morris, G. A. (2016). Aspects of the analytical ultracentrifuge determination of the molar mass distribution of polysaccharides. In Analytical Ultracentrifugation: Instrumentation, Software, and Applications (pp. 375-386). Springer Japan. https://doi.org/10.1007/978-4-431-55985-6_18
Harding, Stephen E. ; Adams, Gary G. ; Gillis, Richard B. ; Almutairi, Fahad M. ; Morris, Gordon A. / Aspects of the analytical ultracentrifuge determination of the molar mass distribution of polysaccharides. Analytical Ultracentrifugation: Instrumentation, Software, and Applications. Springer Japan, 2016. pp. 375-386
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Harding, SE, Adams, GG, Gillis, RB, Almutairi, FM & Morris, GA 2016, Aspects of the analytical ultracentrifuge determination of the molar mass distribution of polysaccharides. in Analytical Ultracentrifugation: Instrumentation, Software, and Applications. Springer Japan, pp. 375-386. https://doi.org/10.1007/978-4-431-55985-6_18

Aspects of the analytical ultracentrifuge determination of the molar mass distribution of polysaccharides. / Harding, Stephen E.; Adams, Gary G.; Gillis, Richard B.; Almutairi, Fahad M.; Morris, Gordon A.

Analytical Ultracentrifugation: Instrumentation, Software, and Applications. Springer Japan, 2016. p. 375-386.

Research output: Chapter in Book/Report/Conference proceedingChapter

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Harding SE, Adams GG, Gillis RB, Almutairi FM, Morris GA. Aspects of the analytical ultracentrifuge determination of the molar mass distribution of polysaccharides. In Analytical Ultracentrifugation: Instrumentation, Software, and Applications. Springer Japan. 2016. p. 375-386 https://doi.org/10.1007/978-4-431-55985-6_18