Aspects of the analytical ultracentrifuge determination of the molar mass distribution of polysaccharides

Stephen E. Harding, Gary G. Adams, Richard B. Gillis, Fahad M. Almutairi, Gordon A. Morris

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Molar mass or ‘molecular weight’ is one of the most fundamental parameters describing a macromolecule. Because of their polydisperse nature, polysaccharides are usually described by distributions of molar mass. SEC-MALS (size exclusion chromatography coupled to multi-angle light scattering) is often a convenient method of choice, but there are many instances where it is unsuitable. Modern AUC (analytical ultracentrifuge) methods provide a valuable alternative - now easier to use than before - and, after briefly reviewing some older procedures, we highlight two recently published and complementary methods, namely, the ‘Extended Fujita’ approach for the analysis of sedimentation velocity data and SEDFIT-MSTAR for the analysis of sedimentation equilibrium data. Nonideality needs to be considered and can be dealt with in a standard way. These methods can also indicate if associative phenomena are present, which can then be quantified using more complex AUC algorithms.

Original languageEnglish
Title of host publicationAnalytical Ultracentrifugation: Instrumentation, Software, and Applications
PublisherSpringer Japan
Pages375-386
Number of pages12
ISBN (Electronic)9784431559856
ISBN (Print)9784431559832
DOIs
Publication statusPublished - 1 Jan 2016

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Harding, S. E., Adams, G. G., Gillis, R. B., Almutairi, F. M., & Morris, G. A. (2016). Aspects of the analytical ultracentrifuge determination of the molar mass distribution of polysaccharides. In Analytical Ultracentrifugation: Instrumentation, Software, and Applications (pp. 375-386). Springer Japan. https://doi.org/10.1007/978-4-431-55985-6_18