Rolling element bearings are one of the critical elements in rotating machinery of energy engineering systems. A defective roller of bearing moves in and out of the load zone during each revolution of the cage. Larger amplitude impact transients (LAITs) are produced when the defective roller passes the load zone centre and the defective area strikes the inner or outer races. A series of LAIT segments with higher signal to noise ratio are separated from a continuous vibration signal according to the bearing geometry and kinematics. In order to eliminate the phase errors between different LAIT segments that can arise from rotational speed fluctuations and roller slippages, unbiased autocorrelation is introduced to align the phases of LAIT segments. The unbiased autocorrelation signals make the ensemble averaging more accurate, and hence, archive enhanced diagnostic signatures, which are denoted as LAIT-AEAs for brevity. The diagnostic method based on LAIT separation and autocorrelation ensemble average (AEA) is evaluated with the datasets captured from real bearings of two different experiment benches. The validation results of the LAIT-AEAs are compared with the squared envelope spectrums (SESs) yielded based on two state-of-the-art techniques of Fast Kurtogram and Autogram.