Bathing suit ichthyosis is caused by transglutaminase-1 deficiency

Evidence for a temperature-sensitive phenotype

Vinzenz Oji, Juliette Mazereeuw Hautier, Bijan Ahvazi, Ingrid Hausser, Karin Aufenvenne, Tatjana Walker, Natalia Seller, Peter M. Steijlen, Wolfgang Küster, Alain Hovnanian, Hans Christian Hennies, Heiko Traupe

Research output: Contribution to journalArticle

54 Citations (Scopus)

Abstract

Bathing suit ichthyosis (BSI) is a striking and unique clinical form of autosomal recessive congenital ichthyosis characterized by pronounced scaling on the bathing suit areas but sparing of the extremities and the central face. Here we report on a series of 10 BSI patients. Our genetic, ultrastructural and biochemical investigations show that BSI is caused by transglutaminase-1 (TGase-1) deficiency. Altogether, we identified 13 mutations in TGM1 - among them seven novel missense mutations and one novel nonsense mutation. Structural modeling for the Tyr276Asn mutation reveals that the residue is buried in the hydrophobic interior of the enzyme and that the hydroxyl side chain of Tyr276 is exposed to solvent in a cavity of the enzyme. Cryosections of healthy skin areas demonstrated an almost normal TGase activity, in contrast to the affected BSI skin, which only showed a cytoplasmic and clearly reduced TGase-1 activity. The distribution of TGase-1 substrates in the epidermis of affected skin corresponded to the situation in TGase-1 deficiency. Interestingly, the expression of TGase-3 and cathepsin D was reduced. Digital thermography validated a striking correlation between warmer body areas and presence of scaling in patients suggesting a decisive influence of the skin temperature. In situ TGase testing in skin of BSI patients demonstrated a marked decrease of enzyme activity when the temperature was increased from 25 to 37°C. We conclude that BSI is caused by TGase-1 deficiency and suggest that it is a temperature-sensitive phenotype.

Original languageEnglish
Pages (from-to)3083-3097
Number of pages15
JournalHuman Molecular Genetics
Volume15
Issue number21
DOIs
Publication statusPublished - 1 Nov 2006
Externally publishedYes

Fingerprint

Ichthyosis
Phenotype
Temperature
Skin
Enzymes
Cathepsin D
Mutation
Skin Temperature
Nonsense Codon
Missense Mutation
transglutaminase 1
Epidermis
Hydroxyl Radical
Molecular Biology
Extremities

Cite this

Oji, V., Hautier, J. M., Ahvazi, B., Hausser, I., Aufenvenne, K., Walker, T., ... Traupe, H. (2006). Bathing suit ichthyosis is caused by transglutaminase-1 deficiency: Evidence for a temperature-sensitive phenotype. Human Molecular Genetics, 15(21), 3083-3097. https://doi.org/10.1093/hmg/ddl249
Oji, Vinzenz ; Hautier, Juliette Mazereeuw ; Ahvazi, Bijan ; Hausser, Ingrid ; Aufenvenne, Karin ; Walker, Tatjana ; Seller, Natalia ; Steijlen, Peter M. ; Küster, Wolfgang ; Hovnanian, Alain ; Hennies, Hans Christian ; Traupe, Heiko. / Bathing suit ichthyosis is caused by transglutaminase-1 deficiency : Evidence for a temperature-sensitive phenotype. In: Human Molecular Genetics. 2006 ; Vol. 15, No. 21. pp. 3083-3097.
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Oji, V, Hautier, JM, Ahvazi, B, Hausser, I, Aufenvenne, K, Walker, T, Seller, N, Steijlen, PM, Küster, W, Hovnanian, A, Hennies, HC & Traupe, H 2006, 'Bathing suit ichthyosis is caused by transglutaminase-1 deficiency: Evidence for a temperature-sensitive phenotype', Human Molecular Genetics, vol. 15, no. 21, pp. 3083-3097. https://doi.org/10.1093/hmg/ddl249

Bathing suit ichthyosis is caused by transglutaminase-1 deficiency : Evidence for a temperature-sensitive phenotype. / Oji, Vinzenz; Hautier, Juliette Mazereeuw; Ahvazi, Bijan; Hausser, Ingrid; Aufenvenne, Karin; Walker, Tatjana; Seller, Natalia; Steijlen, Peter M.; Küster, Wolfgang; Hovnanian, Alain; Hennies, Hans Christian; Traupe, Heiko.

In: Human Molecular Genetics, Vol. 15, No. 21, 01.11.2006, p. 3083-3097.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Bathing suit ichthyosis is caused by transglutaminase-1 deficiency

T2 - Evidence for a temperature-sensitive phenotype

AU - Oji, Vinzenz

AU - Hautier, Juliette Mazereeuw

AU - Ahvazi, Bijan

AU - Hausser, Ingrid

AU - Aufenvenne, Karin

AU - Walker, Tatjana

AU - Seller, Natalia

AU - Steijlen, Peter M.

AU - Küster, Wolfgang

AU - Hovnanian, Alain

AU - Hennies, Hans Christian

AU - Traupe, Heiko

PY - 2006/11/1

Y1 - 2006/11/1

N2 - Bathing suit ichthyosis (BSI) is a striking and unique clinical form of autosomal recessive congenital ichthyosis characterized by pronounced scaling on the bathing suit areas but sparing of the extremities and the central face. Here we report on a series of 10 BSI patients. Our genetic, ultrastructural and biochemical investigations show that BSI is caused by transglutaminase-1 (TGase-1) deficiency. Altogether, we identified 13 mutations in TGM1 - among them seven novel missense mutations and one novel nonsense mutation. Structural modeling for the Tyr276Asn mutation reveals that the residue is buried in the hydrophobic interior of the enzyme and that the hydroxyl side chain of Tyr276 is exposed to solvent in a cavity of the enzyme. Cryosections of healthy skin areas demonstrated an almost normal TGase activity, in contrast to the affected BSI skin, which only showed a cytoplasmic and clearly reduced TGase-1 activity. The distribution of TGase-1 substrates in the epidermis of affected skin corresponded to the situation in TGase-1 deficiency. Interestingly, the expression of TGase-3 and cathepsin D was reduced. Digital thermography validated a striking correlation between warmer body areas and presence of scaling in patients suggesting a decisive influence of the skin temperature. In situ TGase testing in skin of BSI patients demonstrated a marked decrease of enzyme activity when the temperature was increased from 25 to 37°C. We conclude that BSI is caused by TGase-1 deficiency and suggest that it is a temperature-sensitive phenotype.

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