Bridelia ferruginea produces antineuroinflammatory activity through inhibition of nuclear factor-kappa B and p38 MAPK signalling

Olumayokun A. Olajide, Mutalib A. Aderogba, Uchechukwu P. Okorji, Bernd L. Fiebich

Research output: Contribution to journalArticle

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Abstract

Bridelia ferruginea is commonly used in traditional African medicine (TAM) for treating various inflammatory conditions. Extracts from the plant have been shown to exhibit anti-inflammatory property in a number of in vivo models. In this study the influence of B. ferruginea (BFE) on the production of PGE nitrite, and proinflammatory cytokines from LPS-stimulated BV-2 microglia was investigated. The effects of BFE on cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS) protein expressions were evaluated in LPS-activated rat primary microglia. The roles of NF-B and MAPK signalling in the actions of BFE were also investigated. BFE (25-200 g) inhibited the production of PGE nitrite, tumour necrosis factor-α (TNFα), and interleukin-6 (IL-6) as well as COX-2 and iNOS protein expressions in LPS-activated microglial cells. Further studies to elucidate the mechanism of anti-inflammatory action of BFE revealed interference with nuclear translocation of NF-Bp65 through mechanisms involving inhibition of IB degradation. BFE prevented phosphorylation of p38, but not p42/44 or JNK MAPK. It is suggested that Bridelia ferruginea produces anti-inflammatory action through mechanisms involving p38 MAPK and NF-B signalling.

LanguageEnglish
Article number546873
Number of pages8
JournalEvidence-based Complementary and Alternative Medicine
Volume2012
DOIs
Publication statusPublished - 1 Dec 2012

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NF-kappa B
p38 Mitogen-Activated Protein Kinases
Anti-Inflammatory Agents
Microglia
Nitric Oxide Synthase Type II
Cyclooxygenase 2
Nitrites
Prostaglandins E
African Traditional Medicine
Plant Extracts
Interleukin-6
Proteins
Tumor Necrosis Factor-alpha
Phosphorylation
Cytokines

Cite this

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title = "Bridelia ferruginea produces antineuroinflammatory activity through inhibition of nuclear factor-kappa B and p38 MAPK signalling",
abstract = "Bridelia ferruginea is commonly used in traditional African medicine (TAM) for treating various inflammatory conditions. Extracts from the plant have been shown to exhibit anti-inflammatory property in a number of in vivo models. In this study the influence of B. ferruginea (BFE) on the production of PGE nitrite, and proinflammatory cytokines from LPS-stimulated BV-2 microglia was investigated. The effects of BFE on cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS) protein expressions were evaluated in LPS-activated rat primary microglia. The roles of NF-B and MAPK signalling in the actions of BFE were also investigated. BFE (25-200 g) inhibited the production of PGE nitrite, tumour necrosis factor-α (TNFα), and interleukin-6 (IL-6) as well as COX-2 and iNOS protein expressions in LPS-activated microglial cells. Further studies to elucidate the mechanism of anti-inflammatory action of BFE revealed interference with nuclear translocation of NF-Bp65 through mechanisms involving inhibition of IB degradation. BFE prevented phosphorylation of p38, but not p42/44 or JNK MAPK. It is suggested that Bridelia ferruginea produces anti-inflammatory action through mechanisms involving p38 MAPK and NF-B signalling.",
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Bridelia ferruginea produces antineuroinflammatory activity through inhibition of nuclear factor-kappa B and p38 MAPK signalling. / Olajide, Olumayokun A.; Aderogba, Mutalib A.; Okorji, Uchechukwu P.; Fiebich, Bernd L.

In: Evidence-based Complementary and Alternative Medicine, Vol. 2012, 546873, 01.12.2012.

Research output: Contribution to journalArticle

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