Cereal-based biorefinery development: Utilisation of wheat milling by-products for the production of succinic acid

M. Pilar Dorado, Sze Ki Carol Lin, Apostolis Koutinas, Chenyu Du, Ruohang Wang, Colin Webb

Research output: Contribution to journalArticle

80 Citations (Scopus)

Abstract

A novel wheat-based bioprocess for the production of a nutrient-complete feedstock for the fermentative succinic acid production by Actinobacillus succinogenes has been developed. Wheat was fractionated into bran, middlings and flour. The bran fraction, which would normally be a waste product of the wheat milling industry, was used as the sole medium in two solid-state fermentations (SSF) of Aspergillus awamori and Aspergillus oryzae that produce enzyme complexes rich in amylolytic and proteolytic enzymes, respectively. The resulting fermentation solids were then used as crude enzyme sources, by adding directly to an aqueous suspension of milled bran and middlings fractions (wheat flour milling by-products) to generate a hydrolysate containing over 95 g/L glucose, 25 g/L maltose and 300 mg/L free amino nitrogen (FAN). This hydrolysate was then used as the sole medium for A. succinogenes fermentations, which led to the production of 50.6 g/L succinic acid. Supplementation of the medium with yeast extract did not significantly improve succinic acid production though increasing the inoculum concentration to 20% did result in the production of 62.1 g/L succinic acid. Results indicated that A. succinogenes cells were able to utilise glucose and maltose in the wheat hydrolysate for cell growth and succinic acid production. The proposed process could be potentially integrated into a wheat-milling process to upgrade the wheat flour milling by-products (WFMB) into succinic acid, one of the future platform chemicals of a sustainable chemical industry.

LanguageEnglish
Pages51-59
Number of pages9
JournalJournal of Biotechnology
Volume143
Issue number1
DOIs
Publication statusPublished - 10 Aug 2009
Externally publishedYes

Fingerprint

Succinic Acid
Triticum
Byproducts
Acids
Fermentation
Flour
Maltose
Aspergillus
Glucose
Enzymes
Actinobacillus
Waste Products
Aspergillus oryzae
Chemical Industry
Cell growth
Chemical industry
Yeast
Feedstocks
Nutrients
Edible Grain

Cite this

Dorado, M. Pilar ; Lin, Sze Ki Carol ; Koutinas, Apostolis ; Du, Chenyu ; Wang, Ruohang ; Webb, Colin. / Cereal-based biorefinery development : Utilisation of wheat milling by-products for the production of succinic acid. In: Journal of Biotechnology. 2009 ; Vol. 143, No. 1. pp. 51-59.
@article{e1fd103cfef34c6b88934cf7c6d61d26,
title = "Cereal-based biorefinery development: Utilisation of wheat milling by-products for the production of succinic acid",
abstract = "A novel wheat-based bioprocess for the production of a nutrient-complete feedstock for the fermentative succinic acid production by Actinobacillus succinogenes has been developed. Wheat was fractionated into bran, middlings and flour. The bran fraction, which would normally be a waste product of the wheat milling industry, was used as the sole medium in two solid-state fermentations (SSF) of Aspergillus awamori and Aspergillus oryzae that produce enzyme complexes rich in amylolytic and proteolytic enzymes, respectively. The resulting fermentation solids were then used as crude enzyme sources, by adding directly to an aqueous suspension of milled bran and middlings fractions (wheat flour milling by-products) to generate a hydrolysate containing over 95 g/L glucose, 25 g/L maltose and 300 mg/L free amino nitrogen (FAN). This hydrolysate was then used as the sole medium for A. succinogenes fermentations, which led to the production of 50.6 g/L succinic acid. Supplementation of the medium with yeast extract did not significantly improve succinic acid production though increasing the inoculum concentration to 20{\%} did result in the production of 62.1 g/L succinic acid. Results indicated that A. succinogenes cells were able to utilise glucose and maltose in the wheat hydrolysate for cell growth and succinic acid production. The proposed process could be potentially integrated into a wheat-milling process to upgrade the wheat flour milling by-products (WFMB) into succinic acid, one of the future platform chemicals of a sustainable chemical industry.",
keywords = "Actinobacillus succinogenes, Aspergillus awamori and Aspergillus oryzae, Hydrolysis, Platform chemicals, Solid-state fermentation, Wheat flour milling by-products",
author = "Dorado, {M. Pilar} and Lin, {Sze Ki Carol} and Apostolis Koutinas and Chenyu Du and Ruohang Wang and Colin Webb",
year = "2009",
month = "8",
day = "10",
doi = "10.1016/j.jbiotec.2009.06.009",
language = "English",
volume = "143",
pages = "51--59",
journal = "Journal of Biotechnology",
issn = "0168-1656",
publisher = "Elsevier",
number = "1",

}

Cereal-based biorefinery development : Utilisation of wheat milling by-products for the production of succinic acid. / Dorado, M. Pilar; Lin, Sze Ki Carol; Koutinas, Apostolis; Du, Chenyu; Wang, Ruohang; Webb, Colin.

In: Journal of Biotechnology, Vol. 143, No. 1, 10.08.2009, p. 51-59.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Cereal-based biorefinery development

T2 - Journal of Biotechnology

AU - Dorado, M. Pilar

AU - Lin, Sze Ki Carol

AU - Koutinas, Apostolis

AU - Du, Chenyu

AU - Wang, Ruohang

AU - Webb, Colin

PY - 2009/8/10

Y1 - 2009/8/10

N2 - A novel wheat-based bioprocess for the production of a nutrient-complete feedstock for the fermentative succinic acid production by Actinobacillus succinogenes has been developed. Wheat was fractionated into bran, middlings and flour. The bran fraction, which would normally be a waste product of the wheat milling industry, was used as the sole medium in two solid-state fermentations (SSF) of Aspergillus awamori and Aspergillus oryzae that produce enzyme complexes rich in amylolytic and proteolytic enzymes, respectively. The resulting fermentation solids were then used as crude enzyme sources, by adding directly to an aqueous suspension of milled bran and middlings fractions (wheat flour milling by-products) to generate a hydrolysate containing over 95 g/L glucose, 25 g/L maltose and 300 mg/L free amino nitrogen (FAN). This hydrolysate was then used as the sole medium for A. succinogenes fermentations, which led to the production of 50.6 g/L succinic acid. Supplementation of the medium with yeast extract did not significantly improve succinic acid production though increasing the inoculum concentration to 20% did result in the production of 62.1 g/L succinic acid. Results indicated that A. succinogenes cells were able to utilise glucose and maltose in the wheat hydrolysate for cell growth and succinic acid production. The proposed process could be potentially integrated into a wheat-milling process to upgrade the wheat flour milling by-products (WFMB) into succinic acid, one of the future platform chemicals of a sustainable chemical industry.

AB - A novel wheat-based bioprocess for the production of a nutrient-complete feedstock for the fermentative succinic acid production by Actinobacillus succinogenes has been developed. Wheat was fractionated into bran, middlings and flour. The bran fraction, which would normally be a waste product of the wheat milling industry, was used as the sole medium in two solid-state fermentations (SSF) of Aspergillus awamori and Aspergillus oryzae that produce enzyme complexes rich in amylolytic and proteolytic enzymes, respectively. The resulting fermentation solids were then used as crude enzyme sources, by adding directly to an aqueous suspension of milled bran and middlings fractions (wheat flour milling by-products) to generate a hydrolysate containing over 95 g/L glucose, 25 g/L maltose and 300 mg/L free amino nitrogen (FAN). This hydrolysate was then used as the sole medium for A. succinogenes fermentations, which led to the production of 50.6 g/L succinic acid. Supplementation of the medium with yeast extract did not significantly improve succinic acid production though increasing the inoculum concentration to 20% did result in the production of 62.1 g/L succinic acid. Results indicated that A. succinogenes cells were able to utilise glucose and maltose in the wheat hydrolysate for cell growth and succinic acid production. The proposed process could be potentially integrated into a wheat-milling process to upgrade the wheat flour milling by-products (WFMB) into succinic acid, one of the future platform chemicals of a sustainable chemical industry.

KW - Actinobacillus succinogenes

KW - Aspergillus awamori and Aspergillus oryzae

KW - Hydrolysis

KW - Platform chemicals

KW - Solid-state fermentation

KW - Wheat flour milling by-products

UR - http://www.scopus.com/inward/record.url?scp=67650979173&partnerID=8YFLogxK

U2 - 10.1016/j.jbiotec.2009.06.009

DO - 10.1016/j.jbiotec.2009.06.009

M3 - Article

VL - 143

SP - 51

EP - 59

JO - Journal of Biotechnology

JF - Journal of Biotechnology

SN - 0168-1656

IS - 1

ER -