Characterisation of a human bilirubin UDP-glucuronosyltransferase stably expressed in hamster lung fibroblast cell cultures

Lesley Sutherland; Sahidan bin Senafi; Thomas Ebner; Douglas J. Clarke; Brian Burchell

doi:10.1016/0014-5793(92)81266-O

Characterisation of a human bilirubin UDP-glucuronosyltransferase stably expressed in hamster lung fibroblast cell cultures

Lesley Sutherland, Sahidan bin Senafi, Thomas Ebner, Douglas J. Clarke, Brian Burchell

Research output: Contribution to journal › Article › peer-review

34 Citations (Scopus)

Abstract

A cDNA encoding a human bilirubin UDP-glucuronosyltransferase has been isolated and stably expressed in Chinese hamster V79 lung fibroblast cell line. Western blotting of cell homogenates with anti-UGT antibody revealed a highly expressed protein of approx. 55.5 kDa in size. The expressed enzyme specifically catalysed the formation of bilirubin mono- and diglucuronides, and also catalysed the glucuronidation of two phenolic compounds, which are good substrates for other human UGT isoenzymes, at low rates.

Original language	English
Pages (from-to)	161-164
Number of pages	4
Journal	FEBS Letters
Volume	308
Issue number	2
DOIs	https://doi.org/10.1016/0014-5793(92)81266-O
Publication status	Published - 17 Aug 1992
Externally published	Yes

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title = "Characterisation of a human bilirubin UDP-glucuronosyltransferase stably expressed in hamster lung fibroblast cell cultures",

abstract = "A cDNA encoding a human bilirubin UDP-glucuronosyltransferase has been isolated and stably expressed in Chinese hamster V79 lung fibroblast cell line. Western blotting of cell homogenates with anti-UGT antibody revealed a highly expressed protein of approx. 55.5 kDa in size. The expressed enzyme specifically catalysed the formation of bilirubin mono- and diglucuronides, and also catalysed the glucuronidation of two phenolic compounds, which are good substrates for other human UGT isoenzymes, at low rates.",

keywords = "Complementary DNA, Enzyme activity, Eukaryotic expression vector",

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AU - Sutherland, Lesley

AU - Senafi, Sahidan bin

AU - Ebner, Thomas

AU - Clarke, Douglas J.

AU - Burchell, Brian

PY - 1992/8/17

Y1 - 1992/8/17

N2 - A cDNA encoding a human bilirubin UDP-glucuronosyltransferase has been isolated and stably expressed in Chinese hamster V79 lung fibroblast cell line. Western blotting of cell homogenates with anti-UGT antibody revealed a highly expressed protein of approx. 55.5 kDa in size. The expressed enzyme specifically catalysed the formation of bilirubin mono- and diglucuronides, and also catalysed the glucuronidation of two phenolic compounds, which are good substrates for other human UGT isoenzymes, at low rates.

AB - A cDNA encoding a human bilirubin UDP-glucuronosyltransferase has been isolated and stably expressed in Chinese hamster V79 lung fibroblast cell line. Western blotting of cell homogenates with anti-UGT antibody revealed a highly expressed protein of approx. 55.5 kDa in size. The expressed enzyme specifically catalysed the formation of bilirubin mono- and diglucuronides, and also catalysed the glucuronidation of two phenolic compounds, which are good substrates for other human UGT isoenzymes, at low rates.

KW - Complementary DNA

KW - Enzyme activity

KW - Eukaryotic expression vector

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U2 - 10.1016/0014-5793(92)81266-O

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JO - FEBS Letters

JF - FEBS Letters

SN - 0014-5793

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ER -

Characterisation of a human bilirubin UDP-glucuronosyltransferase stably expressed in hamster lung fibroblast cell cultures

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