Characterisation of a human bilirubin UDP-glucuronosyltransferase stably expressed in hamster lung fibroblast cell cultures

Lesley Sutherland, Sahidan bin Senafi, Thomas Ebner, Douglas J. Clarke, Brian Burchell

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31 Citations (Scopus)

Abstract

A cDNA encoding a human bilirubin UDP-glucuronosyltransferase has been isolated and stably expressed in Chinese hamster V79 lung fibroblast cell line. Western blotting of cell homogenates with anti-UGT antibody revealed a highly expressed protein of approx. 55.5 kDa in size. The expressed enzyme specifically catalysed the formation of bilirubin mono- and diglucuronides, and also catalysed the glucuronidation of two phenolic compounds, which are good substrates for other human UGT isoenzymes, at low rates.

Original languageEnglish
Pages (from-to)161-164
Number of pages4
JournalFEBS Letters
Volume308
Issue number2
DOIs
Publication statusPublished - 17 Aug 1992
Externally publishedYes

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