Characterization and molecular analysis of hepatic microsomal UDP-glucuronosyltransferases in the marine fish, Pleuronectes platessa

Douglas J. Clarke, Brian Burchell, Stephen George

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Here we report the results of the first molecular enzymological study of fish UDP-glucuronosyltransferase (UDPGT). UDPGT activities of plaice liver microsomes were greatest for planar phenols and there was low but measurable conjugation of bilirubin, testosterone and androsterone. A highly purified preparation was isolated which possessed activities towards 1-naphthol, bilirubin and testosterone, containing several molecular weight species of Mr 52-56 kDa. On immunoblot analysis these proteins cross-reacted with a polyspecific anti-rat UDPGT antibody, suggesting that a number of plaice UDPGT isoforms with epitopes in common with the corresponding rat enzymes had been purified.

Original languageEnglish
Pages (from-to)105-109
Number of pages5
JournalMarine Environmental Research
Volume24
Issue number1-4
DOIs
Publication statusPublished - 1 Jan 1988
Externally publishedYes

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Pleuronectes
Pleuronectes platessa
molecular analysis
bilirubin
testosterone
marine fish
Fish
Rats
androsterone
1-naphthol
Epitopes
Naphthol
liver
liver microsomes
rats
fish
Antibodies
Liver
phenols
epitopes

Cite this

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Characterization and molecular analysis of hepatic microsomal UDP-glucuronosyltransferases in the marine fish, Pleuronectes platessa. / Clarke, Douglas J.; Burchell, Brian; George, Stephen.

In: Marine Environmental Research, Vol. 24, No. 1-4, 01.01.1988, p. 105-109.

Research output: Contribution to journalArticle

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