TY - JOUR
T1 - Characterization and molecular analysis of hepatic microsomal UDP-glucuronosyltransferases in the marine fish, Pleuronectes platessa
AU - Clarke, Douglas J.
AU - Burchell, Brian
AU - George, Stephen
PY - 1988/1/1
Y1 - 1988/1/1
N2 - Here we report the results of the first molecular enzymological study of fish UDP-glucuronosyltransferase (UDPGT). UDPGT activities of plaice liver microsomes were greatest for planar phenols and there was low but measurable conjugation of bilirubin, testosterone and androsterone. A highly purified preparation was isolated which possessed activities towards 1-naphthol, bilirubin and testosterone, containing several molecular weight species of Mr 52-56 kDa. On immunoblot analysis these proteins cross-reacted with a polyspecific anti-rat UDPGT antibody, suggesting that a number of plaice UDPGT isoforms with epitopes in common with the corresponding rat enzymes had been purified.
AB - Here we report the results of the first molecular enzymological study of fish UDP-glucuronosyltransferase (UDPGT). UDPGT activities of plaice liver microsomes were greatest for planar phenols and there was low but measurable conjugation of bilirubin, testosterone and androsterone. A highly purified preparation was isolated which possessed activities towards 1-naphthol, bilirubin and testosterone, containing several molecular weight species of Mr 52-56 kDa. On immunoblot analysis these proteins cross-reacted with a polyspecific anti-rat UDPGT antibody, suggesting that a number of plaice UDPGT isoforms with epitopes in common with the corresponding rat enzymes had been purified.
UR - http://www.scopus.com/inward/record.url?scp=0023540122&partnerID=8YFLogxK
U2 - 10.1016/0141-1136(88)90264-4
DO - 10.1016/0141-1136(88)90264-4
M3 - Article
AN - SCOPUS:0023540122
VL - 24
SP - 105
EP - 109
JO - Marine Environmental Research
JF - Marine Environmental Research
SN - 0141-1136
IS - 1-4
ER -
