Characterization and molecular analysis of hepatic microsomal UDP-glucuronosyltransferases in the marine fish, Pleuronectes platessa

Douglas J. Clarke; Brian Burchell; Stephen George

doi:10.1016/0141-1136(88)90264-4

Characterization and molecular analysis of hepatic microsomal UDP-glucuronosyltransferases in the marine fish, Pleuronectes platessa

Douglas J. Clarke, Brian Burchell, Stephen George

Research output: Contribution to journal › Article › peer-review

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Abstract

Here we report the results of the first molecular enzymological study of fish UDP-glucuronosyltransferase (UDPGT). UDPGT activities of plaice liver microsomes were greatest for planar phenols and there was low but measurable conjugation of bilirubin, testosterone and androsterone. A highly purified preparation was isolated which possessed activities towards 1-naphthol, bilirubin and testosterone, containing several molecular weight species of M_r 52-56 kDa. On immunoblot analysis these proteins cross-reacted with a polyspecific anti-rat UDPGT antibody, suggesting that a number of plaice UDPGT isoforms with epitopes in common with the corresponding rat enzymes had been purified.

Original language	English
Pages (from-to)	105-109
Number of pages	5
Journal	Marine Environmental Research
Volume	24
Issue number	1-4
DOIs	https://doi.org/10.1016/0141-1136(88)90264-4
Publication status	Published - 1 Jan 1988
Externally published	Yes

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title = "Characterization and molecular analysis of hepatic microsomal UDP-glucuronosyltransferases in the marine fish, Pleuronectes platessa",

abstract = "Here we report the results of the first molecular enzymological study of fish UDP-glucuronosyltransferase (UDPGT). UDPGT activities of plaice liver microsomes were greatest for planar phenols and there was low but measurable conjugation of bilirubin, testosterone and androsterone. A highly purified preparation was isolated which possessed activities towards 1-naphthol, bilirubin and testosterone, containing several molecular weight species of Mr 52-56 kDa. On immunoblot analysis these proteins cross-reacted with a polyspecific anti-rat UDPGT antibody, suggesting that a number of plaice UDPGT isoforms with epitopes in common with the corresponding rat enzymes had been purified.",

author = "Clarke, \{Douglas J.\} and Brian Burchell and Stephen George",

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doi = "10.1016/0141-1136(88)90264-4",

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T1 - Characterization and molecular analysis of hepatic microsomal UDP-glucuronosyltransferases in the marine fish, Pleuronectes platessa

AU - Clarke, Douglas J.

AU - Burchell, Brian

AU - George, Stephen

PY - 1988/1/1

Y1 - 1988/1/1

N2 - Here we report the results of the first molecular enzymological study of fish UDP-glucuronosyltransferase (UDPGT). UDPGT activities of plaice liver microsomes were greatest for planar phenols and there was low but measurable conjugation of bilirubin, testosterone and androsterone. A highly purified preparation was isolated which possessed activities towards 1-naphthol, bilirubin and testosterone, containing several molecular weight species of Mr 52-56 kDa. On immunoblot analysis these proteins cross-reacted with a polyspecific anti-rat UDPGT antibody, suggesting that a number of plaice UDPGT isoforms with epitopes in common with the corresponding rat enzymes had been purified.

AB - Here we report the results of the first molecular enzymological study of fish UDP-glucuronosyltransferase (UDPGT). UDPGT activities of plaice liver microsomes were greatest for planar phenols and there was low but measurable conjugation of bilirubin, testosterone and androsterone. A highly purified preparation was isolated which possessed activities towards 1-naphthol, bilirubin and testosterone, containing several molecular weight species of Mr 52-56 kDa. On immunoblot analysis these proteins cross-reacted with a polyspecific anti-rat UDPGT antibody, suggesting that a number of plaice UDPGT isoforms with epitopes in common with the corresponding rat enzymes had been purified.

UR - https://www.scopus.com/pages/publications/0023540122

U2 - 10.1016/0141-1136(88)90264-4

DO - 10.1016/0141-1136(88)90264-4

M3 - Article

AN - SCOPUS:0023540122

SN - 0141-1136

VL - 24

SP - 105

EP - 109

JO - Marine Environmental Research

JF - Marine Environmental Research

IS - 1-4

ER -

Characterization and molecular analysis of hepatic microsomal UDP-glucuronosyltransferases in the marine fish, Pleuronectes platessa

Abstract

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