Characterization of bradykinin-stimulated release of noradrenaline from cultured bovine adrenal chromaffin cells

P. J. Owen, R. Plevin, M. R. Boarder

Research output: Contribution to journalArticle

47 Citations (Scopus)

Abstract

Bovine adrenal chromaffin cells were purified and maintained in culture. On exposure to bradykinin they released noradrenaline. The characteristics of this stimulus secretion were compared with the response to elevated extracellular potassium. Bradykinin released noradrenaline with an EC50 of about 2 nM, with maximum release (2-3 times control incubations) less than that elicited by high potassium or nicotine. Bradykinin analogs showed the response to have characteristics of a B2 receptor. On continuous exposure to 10 nM bradykinin the rate of release was maximal in the first 1 to 3 min, with a further slower sustained rate of release. The response is mostly, but not completely, dependent on extracellular calcium, and was inhibited by cadmium in the micromolar range. Whereas the potassium-stimulated release was highly sensitive to dihydropyridines, the bradykinin response was not attenuated by dihydropyridine calcium channel antagonists. The results are discussed with respect to stimulus-secretion mechanisms possibly involved in the bradykinin-stimulated release in the light of previous observations characterizing a phosphoinositide response to bradykinin in these cells.

LanguageEnglish
Pages1231-1236
Number of pages6
JournalJournal of Pharmacology and Experimental Therapeutics
Volume248
Issue number3
Publication statusPublished - 1 Mar 1989
Externally publishedYes

Fingerprint

Chromaffin Cells
Bradykinin
Norepinephrine
Potassium
Dihydropyridines
Calcium Channel Blockers
Phosphatidylinositols
Cadmium
Nicotine
Calcium

Cite this

@article{710df163867a44ec982958293593afd9,
title = "Characterization of bradykinin-stimulated release of noradrenaline from cultured bovine adrenal chromaffin cells",
abstract = "Bovine adrenal chromaffin cells were purified and maintained in culture. On exposure to bradykinin they released noradrenaline. The characteristics of this stimulus secretion were compared with the response to elevated extracellular potassium. Bradykinin released noradrenaline with an EC50 of about 2 nM, with maximum release (2-3 times control incubations) less than that elicited by high potassium or nicotine. Bradykinin analogs showed the response to have characteristics of a B2 receptor. On continuous exposure to 10 nM bradykinin the rate of release was maximal in the first 1 to 3 min, with a further slower sustained rate of release. The response is mostly, but not completely, dependent on extracellular calcium, and was inhibited by cadmium in the micromolar range. Whereas the potassium-stimulated release was highly sensitive to dihydropyridines, the bradykinin response was not attenuated by dihydropyridine calcium channel antagonists. The results are discussed with respect to stimulus-secretion mechanisms possibly involved in the bradykinin-stimulated release in the light of previous observations characterizing a phosphoinositide response to bradykinin in these cells.",
author = "Owen, {P. J.} and R. Plevin and Boarder, {M. R.}",
year = "1989",
month = "3",
day = "1",
language = "English",
volume = "248",
pages = "1231--1236",
journal = "Journal of Pharmacology and Experimental Therapeutics",
issn = "0022-3565",
publisher = "American Society for Pharmacology and Experimental Therapeutics",
number = "3",

}

Characterization of bradykinin-stimulated release of noradrenaline from cultured bovine adrenal chromaffin cells. / Owen, P. J.; Plevin, R.; Boarder, M. R.

In: Journal of Pharmacology and Experimental Therapeutics, Vol. 248, No. 3, 01.03.1989, p. 1231-1236.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Characterization of bradykinin-stimulated release of noradrenaline from cultured bovine adrenal chromaffin cells

AU - Owen, P. J.

AU - Plevin, R.

AU - Boarder, M. R.

PY - 1989/3/1

Y1 - 1989/3/1

N2 - Bovine adrenal chromaffin cells were purified and maintained in culture. On exposure to bradykinin they released noradrenaline. The characteristics of this stimulus secretion were compared with the response to elevated extracellular potassium. Bradykinin released noradrenaline with an EC50 of about 2 nM, with maximum release (2-3 times control incubations) less than that elicited by high potassium or nicotine. Bradykinin analogs showed the response to have characteristics of a B2 receptor. On continuous exposure to 10 nM bradykinin the rate of release was maximal in the first 1 to 3 min, with a further slower sustained rate of release. The response is mostly, but not completely, dependent on extracellular calcium, and was inhibited by cadmium in the micromolar range. Whereas the potassium-stimulated release was highly sensitive to dihydropyridines, the bradykinin response was not attenuated by dihydropyridine calcium channel antagonists. The results are discussed with respect to stimulus-secretion mechanisms possibly involved in the bradykinin-stimulated release in the light of previous observations characterizing a phosphoinositide response to bradykinin in these cells.

AB - Bovine adrenal chromaffin cells were purified and maintained in culture. On exposure to bradykinin they released noradrenaline. The characteristics of this stimulus secretion were compared with the response to elevated extracellular potassium. Bradykinin released noradrenaline with an EC50 of about 2 nM, with maximum release (2-3 times control incubations) less than that elicited by high potassium or nicotine. Bradykinin analogs showed the response to have characteristics of a B2 receptor. On continuous exposure to 10 nM bradykinin the rate of release was maximal in the first 1 to 3 min, with a further slower sustained rate of release. The response is mostly, but not completely, dependent on extracellular calcium, and was inhibited by cadmium in the micromolar range. Whereas the potassium-stimulated release was highly sensitive to dihydropyridines, the bradykinin response was not attenuated by dihydropyridine calcium channel antagonists. The results are discussed with respect to stimulus-secretion mechanisms possibly involved in the bradykinin-stimulated release in the light of previous observations characterizing a phosphoinositide response to bradykinin in these cells.

UR - http://www.scopus.com/inward/record.url?scp=0024518535&partnerID=8YFLogxK

UR - http://jpet.aspetjournals.org/content/248/3/1231

M3 - Article

VL - 248

SP - 1231

EP - 1236

JO - Journal of Pharmacology and Experimental Therapeutics

T2 - Journal of Pharmacology and Experimental Therapeutics

JF - Journal of Pharmacology and Experimental Therapeutics

SN - 0022-3565

IS - 3

ER -