TY - CHAP
T1 - Characterization of capsular polysaccharides and their glycoconjugates by hydrodynamic methods
AU - Harding, Stephen E.
AU - Abdelhameed, Ali Saber
AU - Gillis, Richard B.
AU - Morris, Gordon A.
AU - Adams, Gary G.
PY - 2015/1/1
Y1 - 2015/1/1
N2 - Hydrodynamic methods are relevant for the characterization of carbohydrates such as capsular bacterial polysaccharides or glycoconjugates in solution. This chapter focuses on the following hydrodynamic methods: sedimentation velocity analytical ultracentrifugation (SV AUC), dynamic light scattering (DLS), sedimentation equilibrium analytical ultracentrifugation (SE AUC), size exclusion chromatography coupled to multi-angle light scattering (SEC-MALS), and capillary viscometry—intrinsic viscosity measurement. The chapter highlights the general principle of these five methods, describes experimental details, and specifies advances in the last years.
AB - Hydrodynamic methods are relevant for the characterization of carbohydrates such as capsular bacterial polysaccharides or glycoconjugates in solution. This chapter focuses on the following hydrodynamic methods: sedimentation velocity analytical ultracentrifugation (SV AUC), dynamic light scattering (DLS), sedimentation equilibrium analytical ultracentrifugation (SE AUC), size exclusion chromatography coupled to multi-angle light scattering (SEC-MALS), and capillary viscometry—intrinsic viscosity measurement. The chapter highlights the general principle of these five methods, describes experimental details, and specifies advances in the last years.
KW - Capillary viscometry
KW - Dynamic light scattering
KW - Hydrodynamic methods
KW - Intrinsic viscosity measurement
KW - Multi-angle light scattering
KW - Sedimentation equilibrium analytical ultracentrifugation
KW - Sedimentation velocity analytical ultracentrifugation
KW - Size exclusion chromatography
UR - http://www.scopus.com/inward/record.url?scp=84959191567&partnerID=8YFLogxK
U2 - 10.1007/978-1-4939-2874-3_13
DO - 10.1007/978-1-4939-2874-3_13
M3 - Chapter
C2 - 26169743
AN - SCOPUS:84959191567
VL - 1331
T3 - Methods in Molecular Biology
SP - 211
EP - 227
BT - Methods in Molecular Biology
PB - Humana Press Inc.
ER -