Characterization of capsular polysaccharides and their glycoconjugates by hydrodynamic methods

Stephen E. Harding, Ali Saber Abdelhameed, Richard B. Gillis, Gordon A. Morris, Gary G. Adams

Research output: Chapter in Book/Report/Conference proceedingChapter

1 Citation (Scopus)

Abstract

Hydrodynamic methods are relevant for the characterization of carbohydrates such as capsular bacterial polysaccharides or glycoconjugates in solution. This chapter focuses on the following hydrodynamic methods: sedimentation velocity analytical ultracentrifugation (SV AUC), dynamic light scattering (DLS), sedimentation equilibrium analytical ultracentrifugation (SE AUC), size exclusion chromatography coupled to multi-angle light scattering (SEC-MALS), and capillary viscometry—intrinsic viscosity measurement. The chapter highlights the general principle of these five methods, describes experimental details, and specifies advances in the last years.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages211-227
Number of pages17
Volume1331
DOIs
Publication statusPublished - 1 Jan 2015

Publication series

NameMethods in Molecular Biology
Volume1331
ISSN (Print)10643745

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Glycoconjugates
Hydrodynamics
Polysaccharides
Ultracentrifugation
Bacterial Polysaccharides
Viscosity
Gel Chromatography
Carbohydrates
Light

Cite this

Harding, S. E., Abdelhameed, A. S., Gillis, R. B., Morris, G. A., & Adams, G. G. (2015). Characterization of capsular polysaccharides and their glycoconjugates by hydrodynamic methods. In Methods in Molecular Biology (Vol. 1331, pp. 211-227). (Methods in Molecular Biology; Vol. 1331). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-2874-3_13
Harding, Stephen E. ; Abdelhameed, Ali Saber ; Gillis, Richard B. ; Morris, Gordon A. ; Adams, Gary G. / Characterization of capsular polysaccharides and their glycoconjugates by hydrodynamic methods. Methods in Molecular Biology. Vol. 1331 Humana Press Inc., 2015. pp. 211-227 (Methods in Molecular Biology).
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Harding, SE, Abdelhameed, AS, Gillis, RB, Morris, GA & Adams, GG 2015, Characterization of capsular polysaccharides and their glycoconjugates by hydrodynamic methods. in Methods in Molecular Biology. vol. 1331, Methods in Molecular Biology, vol. 1331, Humana Press Inc., pp. 211-227. https://doi.org/10.1007/978-1-4939-2874-3_13

Characterization of capsular polysaccharides and their glycoconjugates by hydrodynamic methods. / Harding, Stephen E.; Abdelhameed, Ali Saber; Gillis, Richard B.; Morris, Gordon A.; Adams, Gary G.

Methods in Molecular Biology. Vol. 1331 Humana Press Inc., 2015. p. 211-227 (Methods in Molecular Biology; Vol. 1331).

Research output: Chapter in Book/Report/Conference proceedingChapter

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Harding SE, Abdelhameed AS, Gillis RB, Morris GA, Adams GG. Characterization of capsular polysaccharides and their glycoconjugates by hydrodynamic methods. In Methods in Molecular Biology. Vol. 1331. Humana Press Inc. 2015. p. 211-227. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-4939-2874-3_13