Cloning and stable expression of a new member of the human liver phenol/bilirubin

UDP-glucuronosyltransferase cDNA family

R. Wooster, L. Sutherland, T. Ebner, D. Clarke, E. O. Da Cruz Silva, B. Burchell

Research output: Contribution to journalArticle

124 Citations (Scopus)

Abstract

A new human liver UDP-glucuronosyltransferase (HlugP4) has been cloned and expressed in cell culture. The expressed enzyme has a molecular mass of 56 kDa and preferentially catalysed the glucuronidation of halogenated and bulky alkyl phenols. The C-terminal half of the sequence (246 amino acids) is 96% identical with the same portion of HlugP1, whereas the N-terminal half of the deduced protein sequences are only 38% identical. These results suggest that the two isoenzymes may be derived from the same gene by differential splicing of the gene product.

Original languageEnglish
Pages (from-to)465-469
Number of pages5
JournalBiochemical Journal
Volume278
Issue number2
DOIs
Publication statusPublished - 1 Sep 1991
Externally publishedYes

Fingerprint

bilirubin glucuronoside glucuronosyltransferase
Cloning
Phenol
Liver
Organism Cloning
Complementary DNA
Genes
Glucuronosyltransferase
Phenols
Molecular mass
Cell culture
Isoenzymes
Amino Acid Sequence
Cell Culture Techniques
Amino Acids
Enzymes
Proteins
UGT1A1 enzyme

Cite this

Wooster, R. ; Sutherland, L. ; Ebner, T. ; Clarke, D. ; Da Cruz Silva, E. O. ; Burchell, B. / Cloning and stable expression of a new member of the human liver phenol/bilirubin : UDP-glucuronosyltransferase cDNA family. In: Biochemical Journal. 1991 ; Vol. 278, No. 2. pp. 465-469.
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Cloning and stable expression of a new member of the human liver phenol/bilirubin : UDP-glucuronosyltransferase cDNA family. / Wooster, R.; Sutherland, L.; Ebner, T.; Clarke, D.; Da Cruz Silva, E. O.; Burchell, B.

In: Biochemical Journal, Vol. 278, No. 2, 01.09.1991, p. 465-469.

Research output: Contribution to journalArticle

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