Cloning and stable expression of a new member of the human liver phenol/bilirubin: UDP-glucuronosyltransferase cDNA family

R. Wooster, L. Sutherland, T. Ebner, D. Clarke, E. O. Da Cruz Silva, B. Burchell

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Abstract

A new human liver UDP-glucuronosyltransferase (HlugP4) has been cloned and expressed in cell culture. The expressed enzyme has a molecular mass of 56 kDa and preferentially catalysed the glucuronidation of halogenated and bulky alkyl phenols. The C-terminal half of the sequence (246 amino acids) is 96% identical with the same portion of HlugP1, whereas the N-terminal half of the deduced protein sequences are only 38% identical. These results suggest that the two isoenzymes may be derived from the same gene by differential splicing of the gene product.

Original languageEnglish
Pages (from-to)465-469
Number of pages5
JournalBiochemical Journal
Volume278
Issue number2
DOIs
Publication statusPublished - 1 Sep 1991
Externally publishedYes

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