Biodiesel is one of the alternative fuels which is renewable and environmentally friendly and can be used in diesel engines with little or no modifications. In the present study, experimental investigations were carried out on the effects of biodiesel types, biodiesel fraction and physical properties on the combustion and performance characteristics of a (compression ignition) CI engine. The experimental work was conducted on a four-cylinder, four-stroke, (direct injection) DI and turbo-charged diesel engine by using biodiesel of waste oil, rapeseed oil and corn oil and normal diesel. Based on the measured parameters, detailed analyses were carried out on cylinder pressure, heat release rate and (brake specific fuel consumption) BSFC. It has been seen that the biodiesel types do not result in any significant differences in peak cylinder pressure and BSFC. The results also clearly indicate that the engine running with biodiesel have slightly higher in-cylinder pressure and heat release rate than the engine running with normal diesel. The BSFC for the engine running with neat biodiesel was higher than the engine running with normal diesel by up to 15%. It is also noticed that the physical properties of the biodiesel affect significantly the performance of the engine.