Commensal-derived OMVs Elicit a Mild Proinflammatory Response in Intestinal Epithelial Cells

Daniel A Patten, Enas Hussein, Scott P. Davies, Paul Humphreys, Andrew Collett

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Under normal physiological conditions, the intestinal immunity remains largely hyporesponsive to the commensal microbiota, yet also retains the inherent ability to rapidly respond to pathogenic antigens. However, immunomodulatory activities of extracellular products from commensal bacteria have been little studied, with previous investigations generally utilizing the live bacterium to study microbiota–epithelial interactions. In this study, we demonstrate that extracellular products of a commensal bacterium, Escherichia coli C25, elicit a moderate release of proinflammatory IL-8 and stimulate transcriptional up-regulation of Toll-like receptors (TLRs) in intestinal epithelial cell lines HT29-19A and Caco-2. Additionally, we show that removal of outer membrane vesicles (OMVs) reduces the proinflammatory effect of secreted products from E. coli C25. Furthermore, we show that isolated OMVs have a dose-dependent proinflammatory effect on intestinal epithelial cells (IECs). Interestingly, a relatively high concentration (40 µg ml−1 protein) of OMVs had no significant regulatory effects on TLR mRNA expression in both cell lines. Finally, we also demonstrate that pre-incubation with E. coli C25-derived OMVs subsequently inhibited the internalization of the bacterium itself in both cell lines. Taken together, our results suggest that commensal-derived extracellular products, in particular OMVs, could significantly contribute to intestinal homeostasis. We also demonstrate a unique interaction between commensal-derived OMVs and host cells.
LanguageEnglish
Pages702-711
Number of pages10
JournalMicrobiology
Volume163
Issue number5
DOIs
Publication statusPublished - 22 May 2017

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Epithelial Cells
Membranes
Bacteria
Toll-Like Receptors
Microbiota
Escherichia coli
Cell Line
Interleukin-8
Immunity
Membrane Proteins
Homeostasis
Up-Regulation
Antigens
Messenger RNA

Cite this

Patten, Daniel A ; Hussein, Enas ; Davies, Scott P. ; Humphreys, Paul ; Collett, Andrew. / Commensal-derived OMVs Elicit a Mild Proinflammatory Response in Intestinal Epithelial Cells. In: Microbiology. 2017 ; Vol. 163, No. 5. pp. 702-711.
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Commensal-derived OMVs Elicit a Mild Proinflammatory Response in Intestinal Epithelial Cells. / Patten, Daniel A; Hussein, Enas; Davies, Scott P.; Humphreys, Paul; Collett, Andrew.

In: Microbiology, Vol. 163, No. 5, 22.05.2017, p. 702-711.

Research output: Contribution to journalArticle

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