Complex relationships between mitochondrial and nuclear DNA preservation in historical DNA extracts

M. G. Campana, D. L. Lister, C. M. Whitten, C. J. Edwards, F. Stock, G. Barker, M. A. Bower

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

'Historical' DNA obtained from specimens preserved in natural history collections has proven useful for addressing a wide variety of questions, such as the spread of domesticated species or changes in genetic diversity. With the development of high-throughput sequencing techniques, there is an increasing focus on acquiring genetic information encoded by single-copy nuclear DNA from historical DNA extracts. The development of efficient techniques to determine the level of nuclear DNA preservation in candidate specimens is necessary to maximize the data obtained from these analyses. Although current evidence suggests that a sample's mitochondrial DNA preservation predicts its nuclear DNA preservation, we show that the relationships between mitochondrial and nuclear DNA preservation are complex.

Original languageEnglish
Pages (from-to)193-202
Number of pages10
JournalArchaeometry
Volume54
Issue number1
Early online date27 May 2011
DOIs
Publication statusPublished - 1 Feb 2012
Externally publishedYes

Fingerprint

candidacy
history
evidence
DNA Preservation
Mitochondrial DNA
Genetic Information
Sequencing

Cite this

Campana, M. G. ; Lister, D. L. ; Whitten, C. M. ; Edwards, C. J. ; Stock, F. ; Barker, G. ; Bower, M. A. / Complex relationships between mitochondrial and nuclear DNA preservation in historical DNA extracts. In: Archaeometry. 2012 ; Vol. 54, No. 1. pp. 193-202.
@article{ed228a7606c943a1a37ba24ba8dfed47,
title = "Complex relationships between mitochondrial and nuclear DNA preservation in historical DNA extracts",
abstract = "'Historical' DNA obtained from specimens preserved in natural history collections has proven useful for addressing a wide variety of questions, such as the spread of domesticated species or changes in genetic diversity. With the development of high-throughput sequencing techniques, there is an increasing focus on acquiring genetic information encoded by single-copy nuclear DNA from historical DNA extracts. The development of efficient techniques to determine the level of nuclear DNA preservation in candidate specimens is necessary to maximize the data obtained from these analyses. Although current evidence suggests that a sample's mitochondrial DNA preservation predicts its nuclear DNA preservation, we show that the relationships between mitochondrial and nuclear DNA preservation are complex.",
keywords = "Historic DNA, Mitochondrial DNA, Nuclear DNA",
author = "Campana, {M. G.} and Lister, {D. L.} and Whitten, {C. M.} and Edwards, {C. J.} and F. Stock and G. Barker and Bower, {M. A.}",
year = "2012",
month = "2",
day = "1",
doi = "10.1111/j.1475-4754.2011.00606.x",
language = "English",
volume = "54",
pages = "193--202",
journal = "Archaeometry",
issn = "0003-813X",
publisher = "Wiley-Blackwell",
number = "1",

}

Complex relationships between mitochondrial and nuclear DNA preservation in historical DNA extracts. / Campana, M. G.; Lister, D. L.; Whitten, C. M.; Edwards, C. J.; Stock, F.; Barker, G.; Bower, M. A.

In: Archaeometry, Vol. 54, No. 1, 01.02.2012, p. 193-202.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Complex relationships between mitochondrial and nuclear DNA preservation in historical DNA extracts

AU - Campana, M. G.

AU - Lister, D. L.

AU - Whitten, C. M.

AU - Edwards, C. J.

AU - Stock, F.

AU - Barker, G.

AU - Bower, M. A.

PY - 2012/2/1

Y1 - 2012/2/1

N2 - 'Historical' DNA obtained from specimens preserved in natural history collections has proven useful for addressing a wide variety of questions, such as the spread of domesticated species or changes in genetic diversity. With the development of high-throughput sequencing techniques, there is an increasing focus on acquiring genetic information encoded by single-copy nuclear DNA from historical DNA extracts. The development of efficient techniques to determine the level of nuclear DNA preservation in candidate specimens is necessary to maximize the data obtained from these analyses. Although current evidence suggests that a sample's mitochondrial DNA preservation predicts its nuclear DNA preservation, we show that the relationships between mitochondrial and nuclear DNA preservation are complex.

AB - 'Historical' DNA obtained from specimens preserved in natural history collections has proven useful for addressing a wide variety of questions, such as the spread of domesticated species or changes in genetic diversity. With the development of high-throughput sequencing techniques, there is an increasing focus on acquiring genetic information encoded by single-copy nuclear DNA from historical DNA extracts. The development of efficient techniques to determine the level of nuclear DNA preservation in candidate specimens is necessary to maximize the data obtained from these analyses. Although current evidence suggests that a sample's mitochondrial DNA preservation predicts its nuclear DNA preservation, we show that the relationships between mitochondrial and nuclear DNA preservation are complex.

KW - Historic DNA

KW - Mitochondrial DNA

KW - Nuclear DNA

UR - http://www.scopus.com/inward/record.url?scp=84855432240&partnerID=8YFLogxK

U2 - 10.1111/j.1475-4754.2011.00606.x

DO - 10.1111/j.1475-4754.2011.00606.x

M3 - Article

VL - 54

SP - 193

EP - 202

JO - Archaeometry

JF - Archaeometry

SN - 0003-813X

IS - 1

ER -