Crystal structure of the PHF8 Jumonji domain, an Nε-methyl lysine demethylase

Wyatt W. Yue, Viktorija Hozjan, Wei Ge, Christoph Loenarz, Christopher D O Cooper, Christopher J. Schofield, Kathryn L. Kavanagh, Udo Oppermann, Michael A. McDonough

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Crystallographic analysis of the catalytic domain of PHD finger protein 8 (PHF8), an Nε-methyl lysine histone demethylase associated with mental retardation and cleft lip/palate, reveals a double-stranded β-helix fold with conserved Fe(II) and cosubstrate binding sites typical of the 2-oxoglutarate dependent oxygenases. The PHF8 active site is highly conserved with those of the FBXL10/11demethylases, which are also selective for the di-/mono-methylated lysine states, but differs from that of the JMJD2 demethylases which are selective for tri-/di-methylated states. The results rationalize the lack of activity for the clinically observed F279S PHF8 variant and they will help to identify inhibitors selective for specific Nε-methyl lysine demethylase subfamilies.

Original languageEnglish
Pages (from-to)825-830
Number of pages6
JournalFEBS Letters
Issue number4
Publication statusPublished - Feb 2010
Externally publishedYes


Cite this

Yue, W. W., Hozjan, V., Ge, W., Loenarz, C., Cooper, C. D. O., Schofield, C. J., ... McDonough, M. A. (2010). Crystal structure of the PHF8 Jumonji domain, an Nε-methyl lysine demethylase. FEBS Letters, 584(4), 825-830.