Damage initiation and progression in the cartilage surface probed by nonlinear optical microscopy

CP Brown, M-A Houle, M. Chen, AJ Price, F Légaré, HS Gill

Research output: Contribution to journalArticle

27 Citations (Scopus)


With increasing interest in treating osteoarthritis at its earliest stages, it has become important to understand the mechanisms by which the disease progresses across a joint. Here, second harmonic generation (SHG) microscopy, coupled with a two-dimensional spring–mass network model, was used to image and investigate the collagen meshwork architecture at the cartilage surface surrounding osteoarthritic lesions. We found that minor weakening of the collagen meshwork leads to the bundling of fibrils at the surface under normal loading. This bundling appears to be an irreversible step in the degradation process, as the stress concentrations drive the progression of damage, forming larger bundles and cracks that eventually form lesions.
Original languageEnglish
Pages (from-to)62-70
Number of pages9
JournalJournal of the Mechanical Behavior of Biomedical Materials
Issue number1
Publication statusPublished - Jan 2012
Externally publishedYes


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