Degradation of polysaccharide hydrogels seeded with bone marrow stromal cells

Shiva H. Jahromi, Liam M. Grover, Jennifer Z. Paxton, Alan M. Smith

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

In order to produce hydrogel cell culture substrates that are fit for the purpose, it is important that the mechanical properties are well understood not only at the point of cell seeding but throughout the culture period. In this study the change in the mechanical properties of three biopolymer hydrogels alginate, low methoxy pectin and gellan gum have been assessed in cell culture conditions. Samples of the gels were prepared encapsulating rat bone marrow stromal cells which were then cultured in osteogenic media. Acellular samples were also prepared and incubated in standard cell culture media. The rheological properties of the gels were measured over a culture period of 28 days and it was found that the gels degraded at very different rates. The degradation occurred most rapidly in the order alginate > Low methoxy pectin > gellan gum. The ability of each hydrogel to support differentiation of bone marrow stromal cells to osteoblasts was also verified by evidence of mineral deposits in all three of the materials. These results highlight that the mechanical properties of biopolymer hydrogels can vary greatly during in vitro culture, and provide the potential of selecting hydrogel cell culture substrates with mechanical properties that are tissue specific.

Original languageEnglish
Pages (from-to)1157-1166
Number of pages10
JournalJournal of the Mechanical Behavior of Biomedical Materials
Volume4
Issue number7
Early online date28 Mar 2011
DOIs
Publication statusPublished - Oct 2011
Externally publishedYes

Fingerprint

Hydrogels
Polysaccharides
Cell culture
Bone
Hydrogel
Degradation
Mechanical properties
Biopolymers
Gels
Alginate
Mineral resources
Osteoblasts
Substrates
Culture Media
Rats
Tissue

Cite this

Jahromi, Shiva H. ; Grover, Liam M. ; Paxton, Jennifer Z. ; Smith, Alan M. / Degradation of polysaccharide hydrogels seeded with bone marrow stromal cells. In: Journal of the Mechanical Behavior of Biomedical Materials. 2011 ; Vol. 4, No. 7. pp. 1157-1166.
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Degradation of polysaccharide hydrogels seeded with bone marrow stromal cells. / Jahromi, Shiva H.; Grover, Liam M.; Paxton, Jennifer Z.; Smith, Alan M.

In: Journal of the Mechanical Behavior of Biomedical Materials, Vol. 4, No. 7, 10.2011, p. 1157-1166.

Research output: Contribution to journalArticle

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