Development and characterization of a bio-hybrid skin-like stretchable electrode

E. Buselli, A. M. Smith, L. M. Grover, A. Levi, R. Allman, V. Mattoli, A. Menciassi, L. Beccai

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

This work presents the design, fabrication and characterization of a polymer based stretchable electrode for cell monitoring. The final goal is the development of innovative bio-hybrid skin-like tactile sensors with mammalian cells as core biological elements; to achieve such aim the enabling technological approach is pursued in this investigation. Electrodes are needed to detect cells response, thus the first step of the bio-hybrid system fabrication is the development of a platform able to record such response and transmit it to the external world. The stretchable electrode is composed by a conductive layer (few of Ti plus 90 nm of Au) on a polymeric substrate (1 mm thick PDMS membrane). Cellular adhesion was verified and cellular response to an induced electrode strain of 1% was detected through fluorescence microscopy. Fluorescence intensities were 104.82 ± 9.64 a.u. and 129.66 ± 13.06 a.u. prior and during electrode strain, respectively. Electromechanical characterization of the stretchable electrode revealed excellent stability and reliability within the 1% strain, which is the operative range identified for the future tactile sensor application. Results showed that the electrode was conductive up to 14% of strain. Furthermore, frequency impedance measurements demonstrated the electrode capability of detecting presence of cells.

Original languageEnglish
Pages (from-to)1676-1680
Number of pages5
JournalMicroelectronic Engineering
Volume88
Issue number8
DOIs
Publication statusPublished - Aug 2011
Externally publishedYes

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Skin
Electrodes
electrodes
cells
Fabrication
fluorescence
fabrication
Fluorescence microscopy
Sensors
impedance measurement
Hybrid systems
Polymers
adhesion
Adhesion
platforms
Fluorescence
Cells
membranes
microscopy
Membranes

Cite this

Buselli, E., Smith, A. M., Grover, L. M., Levi, A., Allman, R., Mattoli, V., ... Beccai, L. (2011). Development and characterization of a bio-hybrid skin-like stretchable electrode. Microelectronic Engineering, 88(8), 1676-1680. https://doi.org/10.1016/j.mee.2010.12.011
Buselli, E. ; Smith, A. M. ; Grover, L. M. ; Levi, A. ; Allman, R. ; Mattoli, V. ; Menciassi, A. ; Beccai, L. / Development and characterization of a bio-hybrid skin-like stretchable electrode. In: Microelectronic Engineering. 2011 ; Vol. 88, No. 8. pp. 1676-1680.
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Buselli, E, Smith, AM, Grover, LM, Levi, A, Allman, R, Mattoli, V, Menciassi, A & Beccai, L 2011, 'Development and characterization of a bio-hybrid skin-like stretchable electrode', Microelectronic Engineering, vol. 88, no. 8, pp. 1676-1680. https://doi.org/10.1016/j.mee.2010.12.011

Development and characterization of a bio-hybrid skin-like stretchable electrode. / Buselli, E.; Smith, A. M.; Grover, L. M.; Levi, A.; Allman, R.; Mattoli, V.; Menciassi, A.; Beccai, L.

In: Microelectronic Engineering, Vol. 88, No. 8, 08.2011, p. 1676-1680.

Research output: Contribution to journalArticle

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AU - Buselli, E.

AU - Smith, A. M.

AU - Grover, L. M.

AU - Levi, A.

AU - Allman, R.

AU - Mattoli, V.

AU - Menciassi, A.

AU - Beccai, L.

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AB - This work presents the design, fabrication and characterization of a polymer based stretchable electrode for cell monitoring. The final goal is the development of innovative bio-hybrid skin-like tactile sensors with mammalian cells as core biological elements; to achieve such aim the enabling technological approach is pursued in this investigation. Electrodes are needed to detect cells response, thus the first step of the bio-hybrid system fabrication is the development of a platform able to record such response and transmit it to the external world. The stretchable electrode is composed by a conductive layer (few of Ti plus 90 nm of Au) on a polymeric substrate (1 mm thick PDMS membrane). Cellular adhesion was verified and cellular response to an induced electrode strain of 1% was detected through fluorescence microscopy. Fluorescence intensities were 104.82 ± 9.64 a.u. and 129.66 ± 13.06 a.u. prior and during electrode strain, respectively. Electromechanical characterization of the stretchable electrode revealed excellent stability and reliability within the 1% strain, which is the operative range identified for the future tactile sensor application. Results showed that the electrode was conductive up to 14% of strain. Furthermore, frequency impedance measurements demonstrated the electrode capability of detecting presence of cells.

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