Development and characterization of a microfluidic model of the tumour microenvironment

Jose M. Ayuso, María Virumbrales-Muñoz, Alodia Lacueva, Pilar M. Lanuza, Elisa Checa-Chavarria, Pablo Botella, Eduardo Fernández, Manuel Doblare, Simon J. Allison, Roger M. Phillips, Julián Pardo, Luis J. Fernandez, Ignacio Ochoa

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

The physical microenvironment of tumours is characterized by heterotypic cell interactions and physiological gradients of nutrients, waste products and oxygen. This tumour microenvironment has a major impact on the biology of cancer cells and their response to chemotherapeutic agents. Despite this, most in vitro cancer research still relies primarily on cells grown in 2D and in isolation in nutrient- and oxygen-rich conditions. Here, a microfluidic device is presented that is easy to use and enables modelling and study of the tumour microenvironment in real-time. The versatility of this microfluidic platform allows for different aspects of the microenvironment to be monitored and dissected. This is exemplified here by real-time profiling of oxygen and glucose concentrations inside the device as well as effects on cell proliferation and growth, ROS generation and apoptosis. Heterotypic cell interactions were also studied. The device provides a live window' into the microenvironment and could be used to study cancer cells for which it is difficult to generate tumour spheroids. Another major application of the device is the study of effects of the microenvironment on cellular drug responses. Some data is presented for this indicating the device's potential to enable more physiological in vitro drug screening.

LanguageEnglish
Article number36086
Number of pages16
JournalScientific Reports
Volume6
DOIs
Publication statusPublished - 31 Oct 2016

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Tumor Microenvironment
Microfluidics
Equipment and Supplies
Lab-On-A-Chip Devices
Oxygen
Cell Communication
Neoplasms
Waste Products
Cellular Microenvironment
Food
Preclinical Drug Evaluations
Cell Biology
Cell Proliferation
Apoptosis
Glucose
Growth
Research
Pharmaceutical Preparations
In Vitro Techniques

Cite this

Ayuso, J. M., Virumbrales-Muñoz, M., Lacueva, A., Lanuza, P. M., Checa-Chavarria, E., Botella, P., ... Ochoa, I. (2016). Development and characterization of a microfluidic model of the tumour microenvironment. Scientific Reports, 6, [36086]. https://doi.org/10.1038/srep36086
Ayuso, Jose M. ; Virumbrales-Muñoz, María ; Lacueva, Alodia ; Lanuza, Pilar M. ; Checa-Chavarria, Elisa ; Botella, Pablo ; Fernández, Eduardo ; Doblare, Manuel ; Allison, Simon J. ; Phillips, Roger M. ; Pardo, Julián ; Fernandez, Luis J. ; Ochoa, Ignacio. / Development and characterization of a microfluidic model of the tumour microenvironment. In: Scientific Reports. 2016 ; Vol. 6.
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Ayuso, JM, Virumbrales-Muñoz, M, Lacueva, A, Lanuza, PM, Checa-Chavarria, E, Botella, P, Fernández, E, Doblare, M, Allison, SJ, Phillips, RM, Pardo, J, Fernandez, LJ & Ochoa, I 2016, 'Development and characterization of a microfluidic model of the tumour microenvironment', Scientific Reports, vol. 6, 36086. https://doi.org/10.1038/srep36086

Development and characterization of a microfluidic model of the tumour microenvironment. / Ayuso, Jose M.; Virumbrales-Muñoz, María; Lacueva, Alodia; Lanuza, Pilar M.; Checa-Chavarria, Elisa; Botella, Pablo; Fernández, Eduardo; Doblare, Manuel; Allison, Simon J.; Phillips, Roger M.; Pardo, Julián; Fernandez, Luis J.; Ochoa, Ignacio.

In: Scientific Reports, Vol. 6, 36086, 31.10.2016.

Research output: Contribution to journalArticle

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Ayuso JM, Virumbrales-Muñoz M, Lacueva A, Lanuza PM, Checa-Chavarria E, Botella P et al. Development and characterization of a microfluidic model of the tumour microenvironment. Scientific Reports. 2016 Oct 31;6. 36086. https://doi.org/10.1038/srep36086