Development of a growth medium suitable for exopolysaccharide production and structural characterisation by Bifidobacterium animalis ssp. lactis AD011

M Alhudhud, P Humphreys, A Laws

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8 Citations (Scopus)

Abstract

Exopolysaccharides (EPSs) produced by Bifidobacteria have received considerable attention due to their ability to modify the rheological and physicochemical properties of dairy products. However, the quantification and characterisation of Bifidobacterial EPS are hampered by the presence of EPS-equivalent (EPS-E) substances in complex media such as Reinforced Clostridial Medium (RCM). This study has developed a medium based on RCM which both supports the growth of Bifidobacterium animalis ssp. lactis AD011 and does not interfere with the quantification and characterisation of the EPS generated. Medium development involved the identification of EPE-E containing components via NMR analysis followed by their removal, substitution or pre-treatment. Both beef extract and casein acid hydrolysate required chemical pre-treatment to remove polysaccharide components before the medium was free of EPS-E materials. Once EPS-E free components had been identified, lactose, glucose and galactose were evaluated as potential carbon sources. Glucose was found to be the optimum carbon source. The final medium composition supported growth to the same extent as RCM providing significant EPS yields and no interferences during analysis.

Original languageEnglish
Pages (from-to)93-98
Number of pages6
JournalJournal of Microbiological Methods
Volume100
Issue number1
DOIs
Publication statusPublished - 1 May 2014

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Growth and Development
Carbon
Glucose
Bifidobacterium
Dairy Products
Lactose
Growth
Galactose
Polysaccharides
Acids
Bifidobacterium animalis
Red Meat
casein hydrolysate

Cite this

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title = "Development of a growth medium suitable for exopolysaccharide production and structural characterisation by Bifidobacterium animalis ssp. lactis AD011",
abstract = "Exopolysaccharides (EPSs) produced by Bifidobacteria have received considerable attention due to their ability to modify the rheological and physicochemical properties of dairy products. However, the quantification and characterisation of Bifidobacterial EPS are hampered by the presence of EPS-equivalent (EPS-E) substances in complex media such as Reinforced Clostridial Medium (RCM). This study has developed a medium based on RCM which both supports the growth of Bifidobacterium animalis ssp. lactis AD011 and does not interfere with the quantification and characterisation of the EPS generated. Medium development involved the identification of EPE-E containing components via NMR analysis followed by their removal, substitution or pre-treatment. Both beef extract and casein acid hydrolysate required chemical pre-treatment to remove polysaccharide components before the medium was free of EPS-E materials. Once EPS-E free components had been identified, lactose, glucose and galactose were evaluated as potential carbon sources. Glucose was found to be the optimum carbon source. The final medium composition supported growth to the same extent as RCM providing significant EPS yields and no interferences during analysis.",
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TY - JOUR

T1 - Development of a growth medium suitable for exopolysaccharide production and structural characterisation by Bifidobacterium animalis ssp. lactis AD011

AU - Alhudhud, M

AU - Humphreys, P

AU - Laws, A

N1 - Copyright © 2014 Elsevier B.V. All rights reserved.

PY - 2014/5/1

Y1 - 2014/5/1

N2 - Exopolysaccharides (EPSs) produced by Bifidobacteria have received considerable attention due to their ability to modify the rheological and physicochemical properties of dairy products. However, the quantification and characterisation of Bifidobacterial EPS are hampered by the presence of EPS-equivalent (EPS-E) substances in complex media such as Reinforced Clostridial Medium (RCM). This study has developed a medium based on RCM which both supports the growth of Bifidobacterium animalis ssp. lactis AD011 and does not interfere with the quantification and characterisation of the EPS generated. Medium development involved the identification of EPE-E containing components via NMR analysis followed by their removal, substitution or pre-treatment. Both beef extract and casein acid hydrolysate required chemical pre-treatment to remove polysaccharide components before the medium was free of EPS-E materials. Once EPS-E free components had been identified, lactose, glucose and galactose were evaluated as potential carbon sources. Glucose was found to be the optimum carbon source. The final medium composition supported growth to the same extent as RCM providing significant EPS yields and no interferences during analysis.

AB - Exopolysaccharides (EPSs) produced by Bifidobacteria have received considerable attention due to their ability to modify the rheological and physicochemical properties of dairy products. However, the quantification and characterisation of Bifidobacterial EPS are hampered by the presence of EPS-equivalent (EPS-E) substances in complex media such as Reinforced Clostridial Medium (RCM). This study has developed a medium based on RCM which both supports the growth of Bifidobacterium animalis ssp. lactis AD011 and does not interfere with the quantification and characterisation of the EPS generated. Medium development involved the identification of EPE-E containing components via NMR analysis followed by their removal, substitution or pre-treatment. Both beef extract and casein acid hydrolysate required chemical pre-treatment to remove polysaccharide components before the medium was free of EPS-E materials. Once EPS-E free components had been identified, lactose, glucose and galactose were evaluated as potential carbon sources. Glucose was found to be the optimum carbon source. The final medium composition supported growth to the same extent as RCM providing significant EPS yields and no interferences during analysis.

KW - Bacteriological Techniques

KW - Bifidobacterium

KW - Culture Media

KW - Magnetic Resonance Spectroscopy

KW - Polysaccharides, Bacterial

KW - Journal Article

U2 - 10.1016/j.mimet.2014.02.021

DO - 10.1016/j.mimet.2014.02.021

M3 - Article

VL - 100

SP - 93

EP - 98

JO - Journal of Microbiological Methods

JF - Journal of Microbiological Methods

SN - 0167-7012

IS - 1

ER -