Do longer sequences improve the accuracy of identification of forensically important Calliphoridae species?

Sara Bortolini, Giorgia Giordani, Fabiola Tuccia, Lara Maistrello, Stefano Vanin

Research output: Contribution to journalArticle

Abstract

Species identification is a crucial step in forensic entomology. In several cases the calculation of the larval age allows the estimation of the minimum Post-Mortem Interval (mPMI). A correct identification of the species is the first step for a correct mPMI estimation. To overcome the difficulties due to the morphological identification especially of the immature stages, a molecular approach can be applied. However, difficulties in separation of closely related species are still an unsolved problem. Sequences of 4 different genes (COI, ND5, EF-1α, PER) of 13 different fly species collected during forensic experiments (Calliphora vicina, Calliphora vomitoria, Lucilia sericata, Lucilia illustris, Lucilia caesar, Chrysomya albiceps, Phormia regina, Cynomya mortuorum, Sarcophaga sp., Hydrotaea sp., Fannia scalaris, Piophila sp., Megaselia scalaris) were evaluated for their capability to identify correctly the species. Three concatenated sequences were obtained combining the four genes in order to verify if longer sequences increase the probability of a correct identification. The obtained results showed that this rule does not work for the species L. caesar and L. illustris. Future works on other DNA regions are suggested to solve this taxonomic issue.

LanguageEnglish
Article numbere5962
Pages1-18
Number of pages18
JournalPeerJ
Volume2018
Issue number12
DOIs
Publication statusPublished - 17 Dec 2018

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Lucilia illustris
Lucilia caesar
Entomology
Sarcophagidae
Muscidae
Peptide Elongation Factor 1
Gene Order
Calliphoridae
Piophila
Diptera
Fannia scalaris
Megaselia scalaris
Hydrotaea
Calliphora vomitoria
Chrysomya albiceps
Genes
forensic entomology
Sarcophaga
Phormia regina
Lucilia sericata

Cite this

Bortolini, S., Giordani, G., Tuccia, F., Maistrello, L., & Vanin, S. (2018). Do longer sequences improve the accuracy of identification of forensically important Calliphoridae species? PeerJ, 2018(12), 1-18. [e5962]. https://doi.org/10.7717/peerj.5962
Bortolini, Sara ; Giordani, Giorgia ; Tuccia, Fabiola ; Maistrello, Lara ; Vanin, Stefano. / Do longer sequences improve the accuracy of identification of forensically important Calliphoridae species?. In: PeerJ. 2018 ; Vol. 2018, No. 12. pp. 1-18.
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Bortolini, S, Giordani, G, Tuccia, F, Maistrello, L & Vanin, S 2018, 'Do longer sequences improve the accuracy of identification of forensically important Calliphoridae species?', PeerJ, vol. 2018, no. 12, e5962, pp. 1-18. https://doi.org/10.7717/peerj.5962

Do longer sequences improve the accuracy of identification of forensically important Calliphoridae species? / Bortolini, Sara; Giordani, Giorgia; Tuccia, Fabiola; Maistrello, Lara; Vanin, Stefano.

In: PeerJ, Vol. 2018, No. 12, e5962, 17.12.2018, p. 1-18.

Research output: Contribution to journalArticle

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