Drosophila circadian rhythms in seminatural environments

Summer afternoon component is not an artifact and requires TrpA1 channels

Edward W. Green, Emma K. O'Callaghan, Celia N. Hansen, Stefano Bastianello, Supriya Bhutani, Stefano Vanin, Rodolfo Costa, Charalambos P. Kyriacou

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

Under standard laboratory conditions of rectangular light/dark cycles and constant warm temperature, Drosophila melanogaster show bursts of morning (M) and evening (E) locomotor activity and a "siesta" in the middle of the day. These M and E components have been critical for developing the neuronal dual oscillator model in which clock gene expression in key cells generates the circadian phenotype. However, under natural European summer conditions of cycling temperature and light intensity, an additional prominent afternoon (A) component that replaces the siesta is observed. This component has been described as an "artifact" of the TriKinetics locomotor monitoring system that is used by many circadian laboratories world wide. Using video recordings, we show that the A component is not an artifact, neither in the glass tubes used in TriKinetics monitors nor in open-field arenas. By studying various mutants in the visual and peripheral and internal thermo-sensitive pathways, we reveal that the M component is predominantly dependent on visual input, whereas the A component requires the internal thermo-sensitive channel transient receptor potential A1 (TrpA1). Knockdown of TrpA1 in different neuronal groups reveals that the reported expression of TrpA1 in clock neurons is unlikely to be involved in generating the summer locomotor profile, suggesting that other TrpA1 neurons are responsible for the A component. Studies of circadian rhythms under seminatural conditions therefore provide additional insights into the molecular basis of circadian entrainment that would otherwise be lost under the usual standard laboratory protocols.

Original languageEnglish
Pages (from-to)8702-8707
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume112
Issue number28
DOIs
Publication statusPublished - 14 Jul 2015

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Transient Receptor Potential Channels
Circadian Rhythm
Artifacts
Drosophila
Myeloma Proteins
Neurons
Video Recording
Temperature
Photoperiod
Locomotion
Drosophila melanogaster
Glass
Phenotype
Gene Expression
Light

Cite this

Green, Edward W. ; O'Callaghan, Emma K. ; Hansen, Celia N. ; Bastianello, Stefano ; Bhutani, Supriya ; Vanin, Stefano ; Costa, Rodolfo ; Kyriacou, Charalambos P. / Drosophila circadian rhythms in seminatural environments : Summer afternoon component is not an artifact and requires TrpA1 channels. In: Proceedings of the National Academy of Sciences of the United States of America. 2015 ; Vol. 112, No. 28. pp. 8702-8707.
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Drosophila circadian rhythms in seminatural environments : Summer afternoon component is not an artifact and requires TrpA1 channels. / Green, Edward W.; O'Callaghan, Emma K.; Hansen, Celia N.; Bastianello, Stefano; Bhutani, Supriya; Vanin, Stefano; Costa, Rodolfo; Kyriacou, Charalambos P.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 112, No. 28, 14.07.2015, p. 8702-8707.

Research output: Contribution to journalArticle

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AU - Green, Edward W.

AU - O'Callaghan, Emma K.

AU - Hansen, Celia N.

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AU - Kyriacou, Charalambos P.

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