Ectopic interleukin-5 receptor expression promotes proliferation without development in a multipotent hematopoietic cell line

A. Pierce, A. D. Whetton, P. J. Owen-Lynch, J. Tavernier, E. Spooncer, T. M. Dexter, C. M. Heyworth

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

The interleukin-5 (IL-5) receptor is a heterodimer that consists of an IL-5 specific α subunit and a common βc chain that is shared with the receptors for granulocyte macrophage colony stimulating factor (GM-CSF) and interleukin-3 (IL-3). In contrast to IL-5, which acts mainly as an eosinophil lineage specific factor in vivo, IL-3 and GM-CSF stimulate the survival, proliferation and development of various hematopoietic cell lineages and also multipotent progenitor cells. IL-5 has little effect on the survival or proliferation of the multipotent stem cell line FDCP-Mix A4 but does promote some eosinophil development. To investigate whether the lineage specificity of IL-5 is due to the restricted expression of the IL-5 receptor α subunit we transfected the FDCP-Mix A4 cells with a retroviral vector containing this α subunit. The ectopic expression of the IL-5 receptor α subunit in the FDCP-Mix cells did not increase the observed eosinophilic development but did stimulate survival and proliferation of the transfected cells when IL-5 was added. IL-5 thus acts like IL-3 in these cells, promoting proliferation and survival. The results suggest that IL-5, whilst having a capacity to promote proliferation, does not influence eosinophilic lineage commitment in these multipotent cells. The results further argue that the observed lineage specificity of IL-5 is probably due to factors in addition to the restricted expression of the IL-5 receptor α subunit.

LanguageEnglish
Pages815-823
Number of pages9
JournalJournal of Cell Science
Volume111
Issue number6
Publication statusPublished - 15 Mar 1998
Externally publishedYes

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Interleukin-5 Receptors
Interleukin-5
Interleukin-5 Receptor alpha Subunit
Cell Line
Interleukin-3
Eosinophils
Granulocyte-Macrophage Colony-Stimulating Factor Receptors
Cell Proliferation
Multipotent Stem Cells
Cell Lineage
Granulocyte-Macrophage Colony-Stimulating Factor
Cell Survival
Stem Cells

Cite this

Pierce, A., Whetton, A. D., Owen-Lynch, P. J., Tavernier, J., Spooncer, E., Dexter, T. M., & Heyworth, C. M. (1998). Ectopic interleukin-5 receptor expression promotes proliferation without development in a multipotent hematopoietic cell line. Journal of Cell Science, 111(6), 815-823.
Pierce, A. ; Whetton, A. D. ; Owen-Lynch, P. J. ; Tavernier, J. ; Spooncer, E. ; Dexter, T. M. ; Heyworth, C. M. / Ectopic interleukin-5 receptor expression promotes proliferation without development in a multipotent hematopoietic cell line. In: Journal of Cell Science. 1998 ; Vol. 111, No. 6. pp. 815-823.
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Pierce, A, Whetton, AD, Owen-Lynch, PJ, Tavernier, J, Spooncer, E, Dexter, TM & Heyworth, CM 1998, 'Ectopic interleukin-5 receptor expression promotes proliferation without development in a multipotent hematopoietic cell line', Journal of Cell Science, vol. 111, no. 6, pp. 815-823.

Ectopic interleukin-5 receptor expression promotes proliferation without development in a multipotent hematopoietic cell line. / Pierce, A.; Whetton, A. D.; Owen-Lynch, P. J.; Tavernier, J.; Spooncer, E.; Dexter, T. M.; Heyworth, C. M.

In: Journal of Cell Science, Vol. 111, No. 6, 15.03.1998, p. 815-823.

Research output: Contribution to journalArticle

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T1 - Ectopic interleukin-5 receptor expression promotes proliferation without development in a multipotent hematopoietic cell line

AU - Pierce, A.

AU - Whetton, A. D.

AU - Owen-Lynch, P. J.

AU - Tavernier, J.

AU - Spooncer, E.

AU - Dexter, T. M.

AU - Heyworth, C. M.

PY - 1998/3/15

Y1 - 1998/3/15

N2 - The interleukin-5 (IL-5) receptor is a heterodimer that consists of an IL-5 specific α subunit and a common βc chain that is shared with the receptors for granulocyte macrophage colony stimulating factor (GM-CSF) and interleukin-3 (IL-3). In contrast to IL-5, which acts mainly as an eosinophil lineage specific factor in vivo, IL-3 and GM-CSF stimulate the survival, proliferation and development of various hematopoietic cell lineages and also multipotent progenitor cells. IL-5 has little effect on the survival or proliferation of the multipotent stem cell line FDCP-Mix A4 but does promote some eosinophil development. To investigate whether the lineage specificity of IL-5 is due to the restricted expression of the IL-5 receptor α subunit we transfected the FDCP-Mix A4 cells with a retroviral vector containing this α subunit. The ectopic expression of the IL-5 receptor α subunit in the FDCP-Mix cells did not increase the observed eosinophilic development but did stimulate survival and proliferation of the transfected cells when IL-5 was added. IL-5 thus acts like IL-3 in these cells, promoting proliferation and survival. The results suggest that IL-5, whilst having a capacity to promote proliferation, does not influence eosinophilic lineage commitment in these multipotent cells. The results further argue that the observed lineage specificity of IL-5 is probably due to factors in addition to the restricted expression of the IL-5 receptor α subunit.

AB - The interleukin-5 (IL-5) receptor is a heterodimer that consists of an IL-5 specific α subunit and a common βc chain that is shared with the receptors for granulocyte macrophage colony stimulating factor (GM-CSF) and interleukin-3 (IL-3). In contrast to IL-5, which acts mainly as an eosinophil lineage specific factor in vivo, IL-3 and GM-CSF stimulate the survival, proliferation and development of various hematopoietic cell lineages and also multipotent progenitor cells. IL-5 has little effect on the survival or proliferation of the multipotent stem cell line FDCP-Mix A4 but does promote some eosinophil development. To investigate whether the lineage specificity of IL-5 is due to the restricted expression of the IL-5 receptor α subunit we transfected the FDCP-Mix A4 cells with a retroviral vector containing this α subunit. The ectopic expression of the IL-5 receptor α subunit in the FDCP-Mix cells did not increase the observed eosinophilic development but did stimulate survival and proliferation of the transfected cells when IL-5 was added. IL-5 thus acts like IL-3 in these cells, promoting proliferation and survival. The results suggest that IL-5, whilst having a capacity to promote proliferation, does not influence eosinophilic lineage commitment in these multipotent cells. The results further argue that the observed lineage specificity of IL-5 is probably due to factors in addition to the restricted expression of the IL-5 receptor α subunit.

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KW - Multipotent

KW - Stochastic

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