Ectopic interleukin-5 receptor expression promotes proliferation without development in a multipotent hematopoietic cell line

A. Pierce, A. D. Whetton, P. J. Owen-Lynch, J. Tavernier, E. Spooncer, T. M. Dexter, C. M. Heyworth

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

The interleukin-5 (IL-5) receptor is a heterodimer that consists of an IL-5 specific α subunit and a common βc chain that is shared with the receptors for granulocyte macrophage colony stimulating factor (GM-CSF) and interleukin-3 (IL-3). In contrast to IL-5, which acts mainly as an eosinophil lineage specific factor in vivo, IL-3 and GM-CSF stimulate the survival, proliferation and development of various hematopoietic cell lineages and also multipotent progenitor cells. IL-5 has little effect on the survival or proliferation of the multipotent stem cell line FDCP-Mix A4 but does promote some eosinophil development. To investigate whether the lineage specificity of IL-5 is due to the restricted expression of the IL-5 receptor α subunit we transfected the FDCP-Mix A4 cells with a retroviral vector containing this α subunit. The ectopic expression of the IL-5 receptor α subunit in the FDCP-Mix cells did not increase the observed eosinophilic development but did stimulate survival and proliferation of the transfected cells when IL-5 was added. IL-5 thus acts like IL-3 in these cells, promoting proliferation and survival. The results suggest that IL-5, whilst having a capacity to promote proliferation, does not influence eosinophilic lineage commitment in these multipotent cells. The results further argue that the observed lineage specificity of IL-5 is probably due to factors in addition to the restricted expression of the IL-5 receptor α subunit.

Original languageEnglish
Pages (from-to)815-823
Number of pages9
JournalJournal of Cell Science
Volume111
Issue number6
Publication statusPublished - 15 Mar 1998
Externally publishedYes

Fingerprint Dive into the research topics of 'Ectopic interleukin-5 receptor expression promotes proliferation without development in a multipotent hematopoietic cell line'. Together they form a unique fingerprint.

  • Cite this