Effect of ATP addition on 1,3-propanediol biosynthesis from glycerol by Klebsiella pneumoniae

Yan Ping Zhang, Zhi Rao, Chen Yu Du, Chun Li, Zhu An Cao

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Biosynthesis of 1,3-propanediol (1,3-PD) attracted much attention recently. Glycerol dehydratase, the key enzyme of the biosynthesis of 1,3-PD from glycerol, tends to undergo mechanism-based inactivation by glycerol during catalysis, and the inactive dehydratase was rapidly reactivated in the presence of AdoCbl, ATP and Mg2+ (or Mn2+). In this paper, the effect of ATP on biosynthesis of 1,3-PD from glycerol by Klebsiella pneumoniae LI was investigated. Although bacterium growth was inhibited with ATP addition in fermentation medium, the capability of 1,3-PD biosynthesis by K. pneumoniae cells per OD was increased. By addition of 0.6-0.8 g/L ATP, the reductive metabolism of glycerol was strengthened and the liter of 1,3-propanediol increased by 50%-70%. The yield of 1,3-PD was also improved, especially in the latter phase of fermentation compared with a rapidly decreased yield in control experiment. For seeing about the simulative effect of ATP on 1,3-PD biosynthesis, rest K. pneumoniae cells were used in this work. It indicated that ATP could reactivate the inactive glycerol dehydratase effectively, and the activity of dehydratase would not decrease observably during repetitious reactivation. ATP was an important factor affecting the catalysis activity of glycerol dehydratase and even the biosynthesis of 1,3-PD from glycerol by K. pneumoniae. Keeping ATP in a higher concentration in the fermentation system was a logical approach to improve 1,3-PD biosynthesis.

Original languageEnglish
Pages (from-to)567-571
Number of pages5
JournalGuocheng Gongcheng Xuebao/The Chinese Journal of Process Engineering
Volume4
Issue number6
Publication statusPublished - 1 Dec 2004
Externally publishedYes

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Adenosinetriphosphate
Biosynthesis
Glycerol
glycerol dehydratase
Adenosine Triphosphate
Fermentation
Hydro-Lyases
Catalysis
1,3-propanediol
Metabolism
Bacteria
Enzymes

Cite this

@article{c13044604548484da822737b51741eca,
title = "Effect of ATP addition on 1,3-propanediol biosynthesis from glycerol by Klebsiella pneumoniae",
abstract = "Biosynthesis of 1,3-propanediol (1,3-PD) attracted much attention recently. Glycerol dehydratase, the key enzyme of the biosynthesis of 1,3-PD from glycerol, tends to undergo mechanism-based inactivation by glycerol during catalysis, and the inactive dehydratase was rapidly reactivated in the presence of AdoCbl, ATP and Mg2+ (or Mn2+). In this paper, the effect of ATP on biosynthesis of 1,3-PD from glycerol by Klebsiella pneumoniae LI was investigated. Although bacterium growth was inhibited with ATP addition in fermentation medium, the capability of 1,3-PD biosynthesis by K. pneumoniae cells per OD was increased. By addition of 0.6-0.8 g/L ATP, the reductive metabolism of glycerol was strengthened and the liter of 1,3-propanediol increased by 50{\%}-70{\%}. The yield of 1,3-PD was also improved, especially in the latter phase of fermentation compared with a rapidly decreased yield in control experiment. For seeing about the simulative effect of ATP on 1,3-PD biosynthesis, rest K. pneumoniae cells were used in this work. It indicated that ATP could reactivate the inactive glycerol dehydratase effectively, and the activity of dehydratase would not decrease observably during repetitious reactivation. ATP was an important factor affecting the catalysis activity of glycerol dehydratase and even the biosynthesis of 1,3-PD from glycerol by K. pneumoniae. Keeping ATP in a higher concentration in the fermentation system was a logical approach to improve 1,3-PD biosynthesis.",
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Effect of ATP addition on 1,3-propanediol biosynthesis from glycerol by Klebsiella pneumoniae. / Zhang, Yan Ping; Rao, Zhi; Du, Chen Yu; Li, Chun; Cao, Zhu An.

In: Guocheng Gongcheng Xuebao/The Chinese Journal of Process Engineering, Vol. 4, No. 6, 01.12.2004, p. 567-571.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Effect of ATP addition on 1,3-propanediol biosynthesis from glycerol by Klebsiella pneumoniae

AU - Zhang, Yan Ping

AU - Rao, Zhi

AU - Du, Chen Yu

AU - Li, Chun

AU - Cao, Zhu An

PY - 2004/12/1

Y1 - 2004/12/1

N2 - Biosynthesis of 1,3-propanediol (1,3-PD) attracted much attention recently. Glycerol dehydratase, the key enzyme of the biosynthesis of 1,3-PD from glycerol, tends to undergo mechanism-based inactivation by glycerol during catalysis, and the inactive dehydratase was rapidly reactivated in the presence of AdoCbl, ATP and Mg2+ (or Mn2+). In this paper, the effect of ATP on biosynthesis of 1,3-PD from glycerol by Klebsiella pneumoniae LI was investigated. Although bacterium growth was inhibited with ATP addition in fermentation medium, the capability of 1,3-PD biosynthesis by K. pneumoniae cells per OD was increased. By addition of 0.6-0.8 g/L ATP, the reductive metabolism of glycerol was strengthened and the liter of 1,3-propanediol increased by 50%-70%. The yield of 1,3-PD was also improved, especially in the latter phase of fermentation compared with a rapidly decreased yield in control experiment. For seeing about the simulative effect of ATP on 1,3-PD biosynthesis, rest K. pneumoniae cells were used in this work. It indicated that ATP could reactivate the inactive glycerol dehydratase effectively, and the activity of dehydratase would not decrease observably during repetitious reactivation. ATP was an important factor affecting the catalysis activity of glycerol dehydratase and even the biosynthesis of 1,3-PD from glycerol by K. pneumoniae. Keeping ATP in a higher concentration in the fermentation system was a logical approach to improve 1,3-PD biosynthesis.

AB - Biosynthesis of 1,3-propanediol (1,3-PD) attracted much attention recently. Glycerol dehydratase, the key enzyme of the biosynthesis of 1,3-PD from glycerol, tends to undergo mechanism-based inactivation by glycerol during catalysis, and the inactive dehydratase was rapidly reactivated in the presence of AdoCbl, ATP and Mg2+ (or Mn2+). In this paper, the effect of ATP on biosynthesis of 1,3-PD from glycerol by Klebsiella pneumoniae LI was investigated. Although bacterium growth was inhibited with ATP addition in fermentation medium, the capability of 1,3-PD biosynthesis by K. pneumoniae cells per OD was increased. By addition of 0.6-0.8 g/L ATP, the reductive metabolism of glycerol was strengthened and the liter of 1,3-propanediol increased by 50%-70%. The yield of 1,3-PD was also improved, especially in the latter phase of fermentation compared with a rapidly decreased yield in control experiment. For seeing about the simulative effect of ATP on 1,3-PD biosynthesis, rest K. pneumoniae cells were used in this work. It indicated that ATP could reactivate the inactive glycerol dehydratase effectively, and the activity of dehydratase would not decrease observably during repetitious reactivation. ATP was an important factor affecting the catalysis activity of glycerol dehydratase and even the biosynthesis of 1,3-PD from glycerol by K. pneumoniae. Keeping ATP in a higher concentration in the fermentation system was a logical approach to improve 1,3-PD biosynthesis.

KW - 1,3-propanediol

KW - ATP

KW - Glycerol

KW - Glycerol dehydratase

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JO - Guocheng Gongcheng Xuebao/The Chinese Journal of Process Engineering

JF - Guocheng Gongcheng Xuebao/The Chinese Journal of Process Engineering

SN - 1009-606X

IS - 6

ER -