Effect of transforming growth factor-β3 on mono and multilayer chondrocytes

Farshid Sefat, Mansour Youseffi, Seyed Ali Khaghani, Chin Fhung Soon, Farideh Javid

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Articular cartilage is an avascular and flexible connective tissue found in joints. It produces a cushioning effect at the joints and provides low friction to protect the ends of the bones from wear and tear/damage. It has poor repair capacity and any injury can result pain and loss of mobility. Transforming growth factor-beta (TGF-β), a cytokine superfamily, regulates cell function, including differentiation and proliferation. Although the function of the TGF-βs in various cell types has been investigated, their function in cartilage repair is as yet not fully understood. The effect of TGF-β3 in biological regulation of primary chondrocyte was investigated in this work. TGF-β3 provided fibroblastic morphology to chondrocytes and therefore overall reduction in cell proliferation was observed. The length of the cells supplemented with TGF-β3 were larger than the cells without TGF-β3 treatment. This was caused by the fibroblast like cells (dedifferentiated chondrocytes) which occupied larger areas compared to cells without TGF-β3 addition. The healing process of the model wound closure assay of chondrocyte multilayer was slowed down by TGF-β3, and this cytokine negatively affected the strength of chondrocyte adhesion to the cell culture surface.

LanguageEnglish
Pages118-126
Number of pages9
JournalCytokine
Volume83
Early online date22 Apr 2016
DOIs
Publication statusPublished - Jul 2016

Fingerprint

Transforming Growth Factors
Chondrocytes
Multilayers
Cartilage
Transforming Growth Factor beta
Repair
Cytokines
Cell proliferation
Fibroblasts
Cell culture
Assays
Bone
Adhesion
Joints
Wear of materials
Tissue
Friction
Wounds and Injuries
Articular Cartilage
Tears

Cite this

Sefat, Farshid ; Youseffi, Mansour ; Khaghani, Seyed Ali ; Soon, Chin Fhung ; Javid, Farideh. / Effect of transforming growth factor-β3 on mono and multilayer chondrocytes. In: Cytokine. 2016 ; Vol. 83. pp. 118-126.
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Effect of transforming growth factor-β3 on mono and multilayer chondrocytes. / Sefat, Farshid; Youseffi, Mansour; Khaghani, Seyed Ali; Soon, Chin Fhung; Javid, Farideh.

In: Cytokine, Vol. 83, 07.2016, p. 118-126.

Research output: Contribution to journalArticle

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