Effects of aldehyde dehydrogenase gene knockout on 1,3-propanediol production by Klebsiella pneumoniae

Yanping Zhang, Chenyu Du, Zhihua Huang, Ming Liu, Zhu'an Cao

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Glycerol can be transformed to 1,3-propanediol (1,3-PD) by Klebsiella pneumoniae with accompanying ethanol accumulation. Aldehyde dehydrogenase (ALDH) is one of the key enzymes for biosynthesis of ethanol, which competes for reducing equivalents NADH with 1,3-propanediol dehydrogenase which catalyzes biosynthesis of 1,3-PD. Based on metabolic analysis, one reasonable method to improve 1,3-PD yield is to inhibit the catalysis of ALDH so that ethanol production could be restrained. In this paper, a homologous recombination vector pUCAT was constructed, in which the ALDH gene of K. pneumoniae was disrupted by inserting tetracycline resistance gene (Tcr). The amplified DNA fragment of 5'ALDH-Tcr-3'ALDH from pUCAT was used to transform K. pneumoniae M5aL and the ALDH gene knockout recombinants were obtained. Comparing with those of the wild type K. pneumoniae M5aL, the ALDH activity of the recombinants were undetected, cell growth was inhibited obviously, ethanol yields were decreased by 43%-53%, 1,3-PD yields and molar conversions from glycerol to 1,3-PD were increased by 27%-42% and 19%-24% respectively.

LanguageEnglish
Pages2686-2692
Number of pages7
JournalHuagong Xuebao/Journal of Chemical Industry and Engineering (China)
Volume57
Issue number11
Publication statusPublished - 1 Nov 2006
Externally publishedYes

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Aldehyde Dehydrogenase
Aldehydes
Genes
Ethanol
1,3-propanediol dehydrogenase
Biosynthesis
Tetracycline
Glycerol
Cell growth
NAD
Catalysis
1,3-propanediol
Oxidoreductases
DNA
Enzymes

Cite this

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title = "Effects of aldehyde dehydrogenase gene knockout on 1,3-propanediol production by Klebsiella pneumoniae",
abstract = "Glycerol can be transformed to 1,3-propanediol (1,3-PD) by Klebsiella pneumoniae with accompanying ethanol accumulation. Aldehyde dehydrogenase (ALDH) is one of the key enzymes for biosynthesis of ethanol, which competes for reducing equivalents NADH with 1,3-propanediol dehydrogenase which catalyzes biosynthesis of 1,3-PD. Based on metabolic analysis, one reasonable method to improve 1,3-PD yield is to inhibit the catalysis of ALDH so that ethanol production could be restrained. In this paper, a homologous recombination vector pUCAT was constructed, in which the ALDH gene of K. pneumoniae was disrupted by inserting tetracycline resistance gene (Tcr). The amplified DNA fragment of 5'ALDH-Tcr-3'ALDH from pUCAT was used to transform K. pneumoniae M5aL and the ALDH gene knockout recombinants were obtained. Comparing with those of the wild type K. pneumoniae M5aL, the ALDH activity of the recombinants were undetected, cell growth was inhibited obviously, ethanol yields were decreased by 43{\%}-53{\%}, 1,3-PD yields and molar conversions from glycerol to 1,3-PD were increased by 27{\%}-42{\%} and 19{\%}-24{\%} respectively.",
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Effects of aldehyde dehydrogenase gene knockout on 1,3-propanediol production by Klebsiella pneumoniae. / Zhang, Yanping; Du, Chenyu; Huang, Zhihua; Liu, Ming; Cao, Zhu'an.

In: Huagong Xuebao/Journal of Chemical Industry and Engineering (China), Vol. 57, No. 11, 01.11.2006, p. 2686-2692.

Research output: Contribution to journalArticle

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AU - Du, Chenyu

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AB - Glycerol can be transformed to 1,3-propanediol (1,3-PD) by Klebsiella pneumoniae with accompanying ethanol accumulation. Aldehyde dehydrogenase (ALDH) is one of the key enzymes for biosynthesis of ethanol, which competes for reducing equivalents NADH with 1,3-propanediol dehydrogenase which catalyzes biosynthesis of 1,3-PD. Based on metabolic analysis, one reasonable method to improve 1,3-PD yield is to inhibit the catalysis of ALDH so that ethanol production could be restrained. In this paper, a homologous recombination vector pUCAT was constructed, in which the ALDH gene of K. pneumoniae was disrupted by inserting tetracycline resistance gene (Tcr). The amplified DNA fragment of 5'ALDH-Tcr-3'ALDH from pUCAT was used to transform K. pneumoniae M5aL and the ALDH gene knockout recombinants were obtained. Comparing with those of the wild type K. pneumoniae M5aL, the ALDH activity of the recombinants were undetected, cell growth was inhibited obviously, ethanol yields were decreased by 43%-53%, 1,3-PD yields and molar conversions from glycerol to 1,3-PD were increased by 27%-42% and 19%-24% respectively.

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